Chemicalsynthesis

BARBARA ALEKSANDRA OSIKOWSKA
A thesis submitted for the degree of
Doctor of Philosophy to the University of London

December 1983

ABSTRACT
The existence of an enzymatic pathway which is capable of sulphating the catecholamine neurotransmitters has been known for over four

contribution to the enzymatic breakdown of these neurotransmitters has generally received less attention than deamination and JKmethylation. The aim of this work was to synthesise authentic dopamine and noradrenaline ^-sulphates, for use as standards in studies on the disposition and metabolism of these important products of dopamine and noradrenaline metabolism.

dopamine 3-0-sulphate, dopamine 4-0-sulphate and dopamine 6-sulphonic

acid.

chemically similar organic acids and could not be distinguished by analytical techiques such as elemental analysis, ultraviolet spectroscopy and infrared spectroscopy, high performance liquid chromatography was employed for the separation and purification of these products, and nuclear magnetic resonance spectroscopy was considered to be the only technique powerful enough to distinguish between these isomers.

using techniques applied for the synthesis and separation of dopamine ^-sulphates.

4. human urine before and following L-dopa administration and in multiple urine samples from a single subject. Both dopamine O-sulphates were
The disposition of dopamine 3- and 4-0-sulphates was investigated in present in urine under physiological conditions and after L-dopa; however, dopamine 3-0-sulphate was the major urinary conjugate in both circumstances.

5. were investigated in rabbit brain, heart, kidney, liver, spleen and small intestine. Dopamine O-sulphates were not detected in any of the tissues with the method of analysis used; both noradrenaline O^-sulphate isomers were present in the above tissues in ratios depending on the animal and tissue investigated. In tissue homogenates of one
The disposition of dopamine and noradrenaline 3- and 4-0-sulphates rabbit, noradrenaline 4-0-sulphate was not detected. 6 . Experiments investigating the in vitro metabolism of dopamine and noradrenaline O-sulphates showed that the aforementioned compounds were substrates for dopamine conversion of dopamine to noradrenaline. following incubation of dopamine -hydroxylase with dopamine

-hydroxylase, the enzyme responsible for the
Two products were formed

O-sulphates, namely free noradrenaline and noradrenaline ()-sulphates. In the presence of active dopamine 3 -hydroxylase in the reaction

effects of a dopamine were also investigated.

-hydroxylase inhibitor on the above reactions
7. Observations in both experimental animals and man suggest that there may be important interindividual variations in the extent of 4-0- sulphation of these amines, the underlying basis for which cannot at present be explained.

ACKNOWLEDGEMENTS
I would like to thank all those whose help in this project made its completion possible.
I am grateful to Professors P.S. Sever and W.S. Peart for allowing me to carry out this work in their departments.
Professor P.S. Sever and Dr. J. Idle provided help, new ideas, continuous encouragement, criticism and friendship.
Dr. F Swinbourne assisted in providing facilities for nuclear magnetic resonance spectroscopy and helped in the interpretation of the spectra.
I would also like to express my thanks to the following:
Mr. R. Mattin for his technical assistance, Miss G. Bartlett for typing the thesis and Drs. R. Unwin and S. Thom for proof-reading.
I am grateful to all my friends at St. Mary's Hospital Medical
School, past and present, whose friendship and encouragement allowed the satisfactory completion of this thesis.
The work was generously supported by the Wellcome Trust.

I reserve final thanks for my husband and my parents, whose support and understanding given freely through the duration of this work I could never fully return.

CONTENTS
Page
ABBREVIATIONS

LIST OF TABLES LIST OF FIGURES

CHAPTER:
INTRODUCTION

... 12

2345

STUDIES ON THE CHEMICAL SULPHONATION OF DOPAMINE AND NORADRENALINE

DISPOSITION OF DOPAMINE O-SULPHATES IN HUMAN URINE

DISPOSITION OF NORADRENALINE 3- AND 4-0-

FURTHER METABOLISM OF DOPAMINE AND

...
137 166
DISCUSSION AND CONCLUDING REMARKS
BIBLIOGRAPHY

... 183

RELEVANT PUBLICATIONS

...

ABBREVIATIONS COMT DA catechol ()-methyl transferase dopamine

DA 3-0-S04

dopamine 3-0-sulphate dopamine 4-0-sulphate
DA 4-0-S0.

-hydroxylase
3,4-dihydroxyphenylglycol dihydroxyphenylalan ine dihydroxyphenylacetic acid high performance liquid chromatography infrared
DHP6 dopa DOPAC h.p.1.c.

I.R.

monoamine oxidase
MAO
3-methoxy-4-hydroxyphenylglycol
MHPG noradrenaline
NA noradrenaline 3-0-sulphate noradrenaline 4-0-sulphate nuclear magnetic resonance
NA 3-0-S04 NA 4-0-S04

n.m.r.

3‘-phosphoadenosine-5'-phosphosulphate phenylethanolamine-Tf-methyltransferase inorganic phosphate
PAPS PNMT PP.

phenolsulphotransferase standard deviation
PST S.D. U.V. VMA ultraviolet 4-hydroxy-3-methoxymandelic acid

LIST OF TABLES 1.1 1.2
Plasma catecholamines (pg/ml)

... 24

Percentage increment in plasma noradrenaline and adrenaline with various stimuli

2.1

2.2
Products of reactions of various sulphonating agents with dopamine at various temperatures

Products of sulphonation of noradrenaline with H^SO^ at various temperatures and time intervals
79

83
2.3 2.4
Products of reaction of chlorosulphonic acid with noradrenaline at various time intervals Products of reaction of chlorosulphonic acid

... ...
85
106 Ill
3.1 3.2 3.3 4.1 4.2 4.3 4.4
Dopamine 0-sulphate content in urine under physiological conditions Dopamine ^-sulphate isomers in urine following oral administration of L-dopa (0.5 g) Recovery of orally administered L-dopa (0.5 g) in 24 h urine as dopamine 3- and 4-0-sulphates Noradrenaline 3- and 4-0-sulphates in rabbit brain tissue

... 112 ... 121

Noradrenaline 3- and 4-0-sulphates in rabbit heart tissue
... ...
123 125
Noradrenaline 3- and 4-0-sulphates in rabbit kidney tissue Noradrenaline 3- and 4-0-sulphates in rabbit

... 127

spleen tissue

Noradrenaline 3- and 4-0-sulphates in rabbit

131 146
Noradrenaline 3- and 4-0-sulphates in rabbit small intestine tissue Dopamine 4-0-sulphate incubation with D$H at different time intervals Dopamine 3-0-sulphate incubation with D$H at
147

147 149 different time intervals Kinetic study of D$H with dopamine as the substrate Kinetic study of D$H with dopamine 4-0-sulphate as the substrate Concentrations of free noradrenaline formed during incubation of dopamine 4-0-sulphate with D3H in the absence or presence of fusaric acid Products formed by the incubation of dopamine 4-0- sulphate with Df3H
151 152 153
Kinetic study of DBH with dopamine 3-0-sulphate as the substrate Concentrations of free noradrenaline formed during incubation of dopamine 3-0-sulphate with DBH in the absence or presence of fusaric acid Products formed by the incubation of dopamine 3-0- sulphate with D^H
155 156
Noradrenaline 4-0-sulphate incubation in the

Noradrenaline 3-0-sulphate incubation in the presence of active D&H

L1ST OF FIGURES

1.2

Metabolic routes of dopamine, noradrenaline and adrenaline

2.1 2.2
Products of chemical sulphonation of dopamine H.p.l.c. chromatogram of products formed during chemical sulphonation of dopamine ...
67

of dopamine 6-sulphonic acid (product I, bottom),

including expanded spectrum (top) ...
13
71 72 75
2.4 2.5 2.6

dopamine 6-sulphonic acid

sulphate (product II) 250 MHz *H n.m.r. spectrum of dopamine 3-0- sulphate (product III)
77

2.7 2.8
Products of chemical sulphonation of noradrenaline H.p.l.c. chromatogram of products formed during chemical sulphonation of noradrenaline by chlorosulphonic acid

89 91

sulphate (product I)
2.10

3.1
250 MHz ^H n.m.r. spectrum of noradrenaline 3-0- sulphate (product II)
93

Standard curve for the estimation of dopamine 3- and 4-0-sulphates

105

H.p.l.c. chromatogram of dopamine £-sulphate

108
H.p.l.c. chromatogram of normal urine containing both dopamine 0-sulphates

H.p.l.c. chromatogram of urine after L-dopa administration, for estimation of dopamine 4-0-

H.p.l.c. chromatogram of urine presented in Fig. 3.4, appropriately iiluted for estimation

110

122

124 126 128 130 132 134 143
H.p.l.c. chromatogram of authentic noradrenaline
••• brain homogenate
•••
••• ••• ••• •••
3- and 4-0-sulphates H.p.l.c. chromatogram of (rabbit 4) H.p.l.c. chromatogram of heart homogenate (rabbit 4)
•«• kidney homogenate
•••
H.p.l.c. chromatogram of (rabbit 4) H.p.l.c. chromatogram of (rabbit 4) spleen homogenate
H.p.l.c. chromatogram of liver homogenate (rabbit 4) H.p.l.c. chromatogram of small intestine homogenate (rabbit 4) H.p.l.c. chromatogram of small intestine homogenate following hydrolysis (rabbit 4) ... Radioenzymatic (PNMT) assay for the determination of free noradrenaline

Chemicalsynthesis

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