Aichi Cancer Center Research Institute Scientific Report 2012 – 2013 Chikusa-ku, Nagoya 464-8681 Japan (The Cover) The Aichi Cancer Center Research Institute Main Building Published by Dr. Taira Kinoshita President Aichi Cancer Center Research Institute 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan Telephone: 052-762-6111 Facsimile: 052-763-5233 Editorial Committee Dr. Masahiro Aoki (Division of Molecular Pathology) Dr. Keiichiro Sakuma (Division of Molecular Pathology) Dr. Hiroshi Kumimoto (Central Service Unit) Dr. Malcolm A. Moore, English Editor Ms. Hiromi Tamaki (Director's Office) Printed by Nagoya University COOP 1 Furoucho, Chikusa-ku, Nagoya 464-0814, Japan Contents Preface Taira Kinoshita 1 Organization of the Aichi Cancer Center Research Institute 2 Scientific Reports Division of Epidemiology and Prevention General Summary 5 1. Descriptive epidemiology 1.1. Differences in incidence and trends of hematologic malignancies in Japan and the United States Chihara, D., Ito, H., Matsuda, M., Shibata, A., Katsumi, A., Nakamura, S., Sobue, T.,Morton, LM., Weisenburger, DD., and Matsuo, K. 2. The hospital-based epidemiologic research program at Aichi Cancer Center (HERPACC) study 2.1. Time to first cigarette and upper aerodigestive tract cancer risk in Japan Matsuo, K., Gallus, S., Negri, E., Kawakita, D., Oze, I., Hosono, S., Ito, H., Hatooka, S., Hasegawa, Y., Shinoda, M., Tajima, K., La Vecchia, C., and Tanaka, H. 2.2. Time to first cigarette and lung cancer risk in Japan Ito, H., Gallus, S., Hosono, S., Oze, I., Fukumoto, K., Yatabe, Y., Hida, T., Mitsudomi, T., Negri, E., Yokoi, K., Tajima, K., La Vecchia, C., Tanaka H., and Matsuo K. 2.3. Polymorphisms in base excision repair genes are associated with endometrial cancer risk among postmenopausal Japanese women Hosono, S., Matsuo, K., Ito, H., Hirose, K., Oze, I., Watanabe, M., Nakanishi, T., Tajima, K., and Tanaka, H. 2.4. Coffee and green tea consumption is associated with upper aerodigestive tract cancer in Japan Oze, I., Matsuo, K., Kawakita, D., Hosono, S., Ito, H., Watanabe, M., Hatooka, S., Hasegawa, Y., Shinoda, M., Tajima, K., and Tanaka, H. 3. Tobacco control 3.1. Factors associated with increasing body weight after smoking cessation therapy in Japan Tanaka, H., Taniguchi, C., Oze, I., Saka, H., Ito, H., Tachibana, K., Tokoro, A., Nozaki, Y., Nakamichi, N., Suzuki, Y., and Suehisa, H. 3.2. Cigarette smoke inhalation and lung cancer risk: Case-control study in Japan Fukumoto, K., Ito, H., Park C., Tanaka, H., Matsuo, K., Tajima, K., and Takezaki, T. Division of Oncological Pathology General Summary 1. Peptide-based non-invasive molecular delivery system by developing novel cancer cell-penetrating peptides (tumor-homing CPPs) for advanced medical i applications Saito, K., Matsushita, M., and Kondo, E 2. Coxsackie and adenovirus receptor – a novel negative regulator of RhoA associated kinase (ROCK) - is a critical regulator of survival and growth of oral squamous carcinoma cells Saito, K., Sakaguchi, M., Iioka, H., Matsui, M., Nakanishi, H., Huh N., and Kondo, E. 3. Anti-tumor impact of p14ARF on gefitinib-resistant non-small cell lung cancers Saito, K., Takigawa, N., Ohtani, N., Iioka, H., Tomita, Y., Ueda, R., Fukuoka, J., Kuwahara, K., Ichihara, E., Kiura, K., and Kondo, E. 4. Lapatinib sensitivities of two novel trastuzumab-resistant HER2 gene-amplified gastric cancer cell lines Oshima, Y., Tanaka, H., Murakami, H., Ito, Y., Furuya, T., Kondo, E., Kodera, Y., and Nakanishi H. 5. LGR5 is a marker of poor prognosis in glioblastoma and is required for survival of brain cancer stem-like cells Nakata, S., Campos, B., Bageritz, J., Bermejo, JL., Becker, N., Engel, F., Acker, T., Momma, S., Herold-Mende, C., Lichter, P., Radlwimmer, B., and Goidts, V. Division of Molecular Oncology General Summary 1. LIM-domain protein AJUBA suppresses malignant mesothelioma cell proliferation via the Hippo signaling cascade Tanaka, I., Osada, H., Fujii, M., Fukatsu, A., Hida, T., Horio, Y., Kondo, Y., Sato, A., Hasegawa, Y., Tsujimura, T., and Y Sekido 2. RASSF3 downregulation increases malignant phenotypes of non-small cell lung cancer Fukatsu, A., Ishiguro, F., Tanaka, I., Kudo, T., Nakagawa, K., Shinjo, K., Kondo, Y., Fujii, M., Hasegawa, Y., Tomizawa, K., Mitsudomi, T., Osada, H., Hata, Y., Sekido, Y. Division of Molecular Medicine General Summary 1. Cell cycle deregulation contributes to acute transformation in chronic type adult T-cell leukemia/lymphoma Yoshida, N., Karube, K., Utsunomiya, A., Tsukasaki, K., Imaizumi, Y., Taira, N., Uike, N., Umino, A., Arita, K., Katayama, M., Tsuzuki, S., Kinoshita, T., Ohshima, K., and Seto, M. 2. Generation of mouse models of lymphoid neoplasms using retroviral gene transduction of in vitro-induced germinal center B and T cells Arita, K., Maeda-Kasugai, Y., Ohshima, K., Tsuzuki, S., Katayama, M., Karube, K., Yoshida, N., Sugiyama, T., and Seto, M. 3. Comprehensive gene expression profiles of NK cell neoplasms indicate vorinostat as an effective drug candidate Karube, K., Tsuzuki, S., Yoshida, N., Arita, K., Kato, H., Katayama, M., Ko, YH., Ohshima, K., Nakamura, S., Kinoshita, T., and Seto, M. 4. Clonal heterogeneity of lymphoid malignancies is related to poor prognosis ii Katayama, M., Tagawa, H., Nakagawa, M., Fukuhara, N., Sivasundaram, S., Takeuchi, I. , Kasugai, Y., Arita, K., Yoshida, N., Kakiuchi, T., Karube, K., Tsuzuki. S, Nakamura, S., and Seto, M. 5. TEL (ETV6)-AML1 (RUNX1) initiates self-renewing fetal pro-B cells in association with a transcriptional program shared with embryonic stem cells in mice Tsuzuki, S., and Seto, M. Division of Immunology General Summary 1. Constitutively active autophagy causes a CTL epitope generation in pancreatic cancer cells Demachi-Okamura, A., and Kuzushima, K. 2. Construction and molecular characterization of a T-cell receptor-like antibody and CAR-T cells specific for minor histocompatibility antigen HA-1H Akatsuka, Y., Inaguma, Y., Akahori, Y., Murayama, Y., Shiraishi, K., Endoh, A., Demachi-Okamura, A., Hiramatsu, K., Nishimura, Y., Takahashi, To., Emi, N., and Kuzushima, K. 3. Pluripotent stem cell-derived proliferating myeloid cells as a possible basis for cellular vaccine cancer immunotherapy Zhang, R., Liu, T., Maki, H., Kuzushima, K., and Uemura, Y. 4. Modification of IL-12p70/osteopontin balance in dendritic cells by ligand activation of invariant NKT cells Zhang, R., Liu, T., Kuzushima, K., and Uemura, Y. Division of Virology General Summary 1. Interaction between Basic Residues of Epstein-Barr Virus EBNA1 Protein and Cellular Chromatin Mediates Viral Plasmid Maintenance Kanda, T. and Tsurumi, T. 2. Contribution of Myocyte Enhancer Factor 2 (MEF2) Family Transcription Factors to BZLF1 Expression in Epstein-Barr virus Reactivation from Latency Murata, T. and Tsurumi, T. 3. Heat shock protein 90 inhibitors repress latent membrane protein 1 (LMP1) expression and proliferation of Epstein-Barr virus-positive natural killer cell lymphoma cells Murata, T. and Tsurumi, T. 4. Different Distributions of Epstein-Barr Virus Early and Late Gene Transcripts within Viral Replication Compartments Sugimoto, A., Sato, Y., Murata, T., Kanda, T., and Tsurumi, T. 5. Nuclear Transport of Epstein-Barr Virus DNA Polymerase is Dependent on the BMRF1 Polymerase Processivity Factor and Molecular Chaperone Hsp90 Kawashima, D., Murata, T., Kanda, T., and Tsurumi T. 6. Epstein-Barr Virus Deubiquitinase Downregulates TRAF6-mediated NF-κB Signaling during Productive Replication Saito, S., Murata, T., Kanda, T., and Tsurumi, T. iii 7. Pin1 Interacts with the Epstein-Barr Virus DNA Polymerase Catalytic Subunit and Regulates Viral DNA Replication Narita, Y., Murata, T., Kanda, T., and Tsurumi, T. 8. HLA-restricted Presentation of WT1 Tumor Antigen in B-lymphoblastoid Cell Lines Established using a Maxi-EBV System Kanda, T., Kuzushima, K., and Tsurumi, T. 9. Epigenetic Histone Modification of the Epstein-Barr Virus BZLF1 Promoter during Latency and Reactivation in Raji Cells Murata, T., Kondo, Y., and Tsurumi, T. Division of Molecular Pathology General Summary 1. c-Myc and CDX2 mediate E-selectin ligand glycan expression in colon cancer cells undergoing EMT Sakuma, K., Kannagi, R., and Aoki, M. 2. CDX Transcription Factors Positively Regulate Expression of PLEKHG1 in Intestinal Epithelium Aoki, M., Fujishita, T., and Taketo, MM. 3. Roles of the mTOR signaling in intestinal adenocarcinoma formation in cis-Apc/Smad4 mutant mice Fujishita, T., Taketo, MM., and Aoki, M. Division of Biochemistry General Summary 1. Emerging roles of ubiquitin-proteasome machinery in formation of primary cilia Kasahara, K., Era, S., Kawamoto, E., Kawakami, Y., Kiyono, T., Kawamura, Y., Goshima, N., and Inagaki, M. 2. Cytokinetic failure induces aneuploidy and aging in vimentin phosphorylation deficient mice Tanaka, Hir., Matsuyama, M., Inoko, A., Goto, H., Yonemura, S., Kobori, K., Tanigawa, N., Hayashi, Y., Kondo, E., Itohara, S., Izawa, I. and Inagaki, M. 3. Perspective of Aurora A kinase as a therapeutic target for cancer Goto, H., Watanabe, N., Kobori, K., Inoko, A., Mochizuki, H., Togashi, T., Kisu, Y., Kawamura, Y., Kawakami, Y., Goshima, N., and Inagaki, M. 4. Novel cell cycle regulation through centrosomes Inoko, A., Inaba, H., He, D., Goto, H., Hayashi, Y., Izawa, I., Urano, T., Yonemura, S., Kiyono, T., Goshima, N., and Inagaki, M. 5. Interaction of Cell Polarity Regulator Scribble with Multidrug Resistance Protein 4 (MRP4/ABCC4) Izawa, I., Hayashi, Y., and Inagaki, M. Division of Epigenomics General Summary 1. The PRC2