SAN TA C RUZ BI OTEC HNOL OG Y, INC . TM4SF5 (W-12): sc-165713 BACKGROUND PRODUCT TM4SF5 (transmembrane 4 L6 family member 5) is a 197 amino acid multi- Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide pass membrane protein that belongs to the transmembrane 4 superfamily (also and 0.1% gelatin. known as the tetraspanin family) of cell surface proteins that regulate cell Blocking peptide available for competition studies, sc-165713 P, (100 µg development, activation, growth and motility. Expressed in normal intestinal peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). tissue and overexpressed in tumor cells (including hepatocarcinoma, gastric and colon cancer cells), TM4SF5 is a glycoprotein that is thought to play a APPLICATIONS role in cellular proliferation and growth factor signaling. When upregulated, TM4SF5 can cause erratic and uncontrolled cell growth of human cancer cells, TM4SF5 (W-12) is recommended for detection of TM4SF5 of human origin indicating a crucial role for TM4SF5 in tumor progression and metastasis. The by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), gene encoding TM4SF5 maps to human chromosome 17, which comprises immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and over 2.5% of the human genome and encodes over 1,200 genes. solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000); non cross-reactive with other TM4SF family members. REFERENCES Suitable for use as control antibody for TM4SF5 siRNA (h): sc-94048, 1. Müller-Pillasch, F., et al. 1998. Identification of a new tumour-associated TM4SF5 shRNA Plasmid (h): sc-94048-SH and TM4SF5 shRNA (h) Lentiviral antigen TM4SF5 and its expression in human cancer. Gene 208: 25-30. Particles: sc-94048-V. 2. Wright, M.D., et al. 2000. The L6 membrane proteins—a new four-trans - Molecular Weight of TM4SF5: 21 kDa. membrane superfamily. Protein Sci. 9: 1594-1600. RECOMMENDED SECONDARY REAGENTS 3. Kaneko, R., et al. 2001. Amount of expression of the tumor-associated anti - gen L6 gene and transmembrane 4 superfamily member 5 gene in gastric To ensure optimal results, the following support (secondary) reagents are cancers and gastric mucosa. Am. J. Gastroenterol. 96: 3457-3458. recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey 4. Berditchevski, F. 2001. Complexes of tetraspanins with integrins: more than anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ meets the eye. J. Cell Sci. 114: 4143-4151. Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: 5. Pascual-Le Tallec, L., et al. 2002. Identification of genes associated with sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- the corticotroph phenotype in bronchial carcinoid tumors. J. Clin. Endocrinol. rescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100- Metab. 87: 5015-5022. 1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941. 6. Online Mendelian Inheritance in Man, OMIM™. 2002. Johns Hopkins University, Baltimore, MD. MIM Number: 604657. World Wide Web URL: RESEARCH USE http://www.ncbi.nlm.nih.gov/omim/ For research use only, not for use in diagnostic procedures. 7. Lee, S.Y., et al. 2006. Focal adhesion and actin organization by a cross-talk of TM4SF5 with integrin α2 are regulated by serum treatment. Exp. Cell PROTOCOLS Res. 312: 2983-2999. See our web site at www.scbt.com or our catalog for detailed protocols and 8. Lee, S.A., et al. 2008. Tetraspanin TM4SF5 mediates loss of contact inhi bi - support products. tion through epithelial-mesenchymal transition in human hepatocarcino ma. J. Clin. Invest. 118: 1354-1366. CHROMOSOMAL LOCATION Genetic locus: TM4SF5 (human) mapping to 17p13.2. SOURCE TM4SF5 (W-12) is an affinity purified goat polyclonal antibody raised against a peptide mapping within a C-terminal extracellular domain of TM4SF5 of human origin. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.