Study of functional dye Koshi high school Erina Mizutani Shiori Endo antibacterial Dyeing cloth by properties food which have antibacterial properties. food Test 1 Purpose onion umeboshi azuki bean grape juice coffee honey karashi wasabi Do you know umeboshi？ traditional Japanese food with rice! Method 5%Alum onion grape PDA Culture Plate P・・・potato D・・・dextrose A・・・agar Result onion AP × Color × umeboshi AP ○ Color ○ azuki beans AP ○ Color ○ grape AP × Color × coffee AP × Color × honey AP × Color × karashi AP × Color × wasabi AP × Color × antibacterial color properties onion × × umeboshi ○ ○ azuki beans ○ ○ grape × × coffee × × honey × × karashi × × wasabi ○ × Consideration We think the antibacterial properties are involved in the color change. Test 2 Purpose We extracted Umeboshi’s color. Method ①Mash Umeboshi into a paste. ②Put the samples in ethanol,methanol,hexane,and chloroform. ③Shake them. Umeboshi paste Result Solvent ↓ red Solvent ↓ methanol yellow ethanol Solvent ↓ No change hexane chloroform Consideration ・Ethanol has a high polarity. ・We can analyze the entracted color with chromatography. Summary ①The cloth dyed with Umeboshi, Azuki beans, and Wasabi have antibacterial properties. ②We think the antibacterial properties are involved the color change. ③Ethanol is good at extracting Umeboshi’s color. Issues ・We have to analyze umeboshi’s color, making sure to single out that color, and reveal a relationship between its color and its antibacterial properties. color antibacterial properties ・We have to extract and analyze the color from “red shiso”, and reveal that it also has antibacterial properties. We think・・・ The clothｓ don’t have The clothｓ have antibacterial properties. antibacterial properties. germ detector antibacterial germ detector properties Bibliography http://jccu.coop/kodomo_lamd/freestudy/kusakizome/ kusa_02.html 「草木染めに挑戦しよう！」 http://slism.jp/calorie/106095/ 「カロリーSlism」 http://www.ciaojapan.org/manual_tanekin_kinsibaiyo. html 「キノコ栽培サイト」 Thank you very much for your kind attention!!! .