Supplemental table 1 Proteins whose content is significantly modified in hepatocytes by Aramchol. See text for experimental details Fold change Accession Protein name P value (Log2) achol/ctrl Fibrosis 6.29E- 0.13 P20152 Vimentin GN = Vim PE = 1 SV = 3 04 2.02E- 0.45 Q8BTM8 Filamin-A GN = Flna PE = 1 SV = 5 02 1.13E- 0.50 Q80X90 Filamin-B GN = Flnb PE = 1 SV = 3 02 Keratin type I cytoskeletal 18 GN = 4.89E- 0.78 P05784 Krt18 PE = 1 SV = 5 02 Translation RNA-binding protein 27 GN = Rbm27 1.08E- Q5SFM8 0.32 PE = 1 SV = 3 02 60S ribosomal protein L22-like 1 3.86E- Q9D7S7 0.42 GN = Rpl22l1 PE = 1 SV = 1 02 Trifunctional purine biosynthetic 2.33E- Q64737 protein adenosine-3 GN = Gart PE = 1 0.50 03 SV = 3 Eukaryotic translation initiation factor 3 3.10E- P23116 0.59 subunit A GN = Eif3a PE = 1 SV = 5 03 Glycine--tRNA ligase GN = Gars PE = 1 6.87E- Q9CZD3 0.63 SV = 1 03 Eukaryotic translation initiation factor 3 4.13E- Q8JZQ9 0.67 subunit B GN = Eif3b PE = 1 SV = 1 02 Elongation factor 1-gamma GN = Eef1g 2.47E- Q9D8N0 0.71 PE = 1 SV = 3 02 Q922B2 Aspartate--tRNA ligase cytoplasmic 2.02E- 0.72 GN = Dars PE = 1 SV = 2 02 Arginine--tRNA ligase cytoplasmic 1.47E- Q9D0I9 0.72 GN = Rars PE = 1 SV = 2 02 RNA-binding protein 25 GN = Rbm25 1.28E- B2RY56 1.92 PE = 1 SV = 2 02 Eukaryotic translation initiation factor 4 7.92E- Q80XI3 3.90 gamma 3 GN = Eif4g3 PE = 1 SV = 2 03 Lipid metabolism Prostaglandin reductase 1 GN = Ptgr1 4.55E- Q91YR9 0.42 PE = 1 SV = 2 02 3.09E- Q9DBG5 Perilipin-3 GN = Plin3 PE = 1 SV = 1 0.51 02 Acyl-CoA dehydrogenase family 3.98E- Q80XL6 1.21 member 11 GN = Acad11 PE = 1 SV = 2 02 Sterol 26-hydroxylase mitochondrial 2.98E- Q9DBG1 1.31 GN = Cyp27a1 PE = 1 SV = 1 02 Carnitine O-palmitoyltransferase 1 3.02E- P97742 1.32 liver isoform GN=Cpt1a PE=1 SV=4 02 Short-chain specific acyl-CoA 3.13E- Q07417 dehydrogenase mitochondrial GN = 1.36 02 Acads PE = 1 SV = 2 Hydroxymethylglutaryl-CoA synthase 2.19E- P54869 mitochondrial GN = Hmgcs2 PE = 1 SV 1.37 03 = 2 Trifunctional enzyme subunit beta 1.40E- Q99JY0 mitochondrial GN = Hadhb PE = 1 SV 1.38 02 = 1 Peroxisomal multifunctional enzyme 9.10E- P51660 1.39 type 2 GN = Hsd17b4 PE = 1 SV = 3 03 Delta(24)-sterol reductase GN = Dhcr24 2.69E- Q8VCH6 1.41 PE = 1 SV = 1 02 Very long-chain specific acyl-CoA 2.65E- P50544 dehydrogenase mitochondrial GN = 1.42 02 Acadvl PE = 1 SV = 3 Trifunctional enzyme subunit alpha 1.78E- Q8BMS1 mitochondrial GN = Hadha PE = 1 SV 1.42 02 = 1 Methylmalonyl-CoA mutase 1.21E- P16332 1.43 mitochondrial GN = Mut PE = 1 SV = 2 02 Peroxisomal acyl-coenzyme A oxidase 1 1.83E- Q9R0H0 1.43 GN = Acox1 PE = 1 SV = 5 02 Lipoamide acyltransferase component of branched-chain alpha-keto acid 4.62E- P53395 1.45 dehydrogenase complex mitochondrial 02 GN = Dbt PE = 1 SV = 2 Acyl-coenzyme A synthetase ACSM3 2.96E- Q3UNX5 mitochondrial GN = Acsm3 PE = 1 SV 1.46 02 = 2 Malate dehydrogenase mitochondrial 6.58E- P08249 1.46 GN = Mdh2 PE = 1 SV = 3 03 Long-chain specific acyl-CoA 4.07E- P51174 dehydrogenase mitochondrial GN = 1.47 02 Acadl PE = 1 SV = 2 Methylmalonate-semialdehyde dehydrogenase [acylating] 2.26E- Q9EQ20 1.47 mitochondrial GN = Aldh6a1 PE = 1 SV 04 = 1 D-beta-hydroxybutyrate 1.38E- Q80XN0 dehydrogenase mitochondrial GN = 1.50 02 Bdh1 PE = 1 SV = 2 Long-chain-fatty-acid--CoA ligase 1 1.94E- P41216 1.56 GN = Acsl1 PE = 1 SV = 2 02 Bile acyl-CoA synthetase GN = Slc27a5 2.76E- Q4LDG0 1.56 PE = 1 SV = 2 04 Acetyl-CoA acetyltransferase 2.82E- Q8QZT1 mitochondrial GN = Acat1 PE = 1 SV = 1.62 02 1 Cytochrome P450 2C70 GN = Cyp2c70 4.63E- Q91W64 1.63 PE = 1 SV = 2 02 Cytochrome P450 2D26 GN = Cyp2d26 2.50E- Q8CIM7 1.64 PE = 1 SV = 1 02 Apolipoprotein B-100 GN = Apob PE = 3.50E- E9Q414 1.65 1 SV = 1 02 Cytochrome b-c1 complex subunit 1 8.45E- Q9CZ13 mitochondrial GN = Uqcrc1 PE = 1 SV 1.68 04 = 2 1-acyl-sn-glycerol-3-phosphate 7.21E- Q8K3K7 acyltransferase beta GN = Agpat2 PE = 1.69 03 1 SV = 1 7-dehydrocholesterol reductase 4.41E- O88455 1.69 GN = Dhcr7 PE = 1 SV = 1 02 Long-chain-fatty-acid--CoA ligase 5 9.83E- Q8JZR0 1.70 GN = Acsl5 PE = 1 SV = 1 04 Very long-chain acyl-CoA synthetase 2.59E- O35488 1.72 GN = Slc27a2 PE = 1 SV = 2 03 Cytochrome P450 4V2 GN = Cyp4v2 PE 3.39E- Q9DBW0 1.73 = 1 SV = 1 02 Cytochrome P450 2D9 GN = Cyp2d9 1.44E- P11714 1.85 PE = 1 SV = 2 02 Acyl-CoA synthetase family member 2 1.39E- Q8VCW8 mitochondrial GN = Acsf2 PE = 1 SV = 1.85 04 1 Q9R1J0 Sterol-4-alpha-carboxylate 3- 3.84E- 1.92 dehydrogenase decarboxylating GN = 02 Nsdhl PE = 1 SV = 1 Acyl-CoA-binding domain-containing 1.82E- Q5XG73 2.08 protein 5 GN = Acbd5 PE = 1 SV = 1 02 Apolipoprotein E GN = Apoe PE = 1 SV 8.64E- P08226 2.59 = 2 03 Isovaleryl-CoA dehydrogenase 7.33E- Q9JHI5 2.92 mitochondrial GN = Ivd PE = 1 SV = 1 04 Sequestosome-1 GN = Sqstm1 PE = 1 2.90E- Q64337 5.01 SV = 1 02 1 Carbon metabolism 5-formyltetrahydrofolate cyclo-ligase 2.11E- Q9D110 0.44 GN = Mthfs PE = 2 SV = 2 02 Betaine--homocysteine S- 1.54E- O35490 methyltransferase 1 GN = Bhmt PE = 1 1.31 02 SV = 1 Sarcosine dehydrogenase 8.73E- Q99LB7 mitochondrial 1.33 04 GN = Sardh PE = 1 SV = 1 Dimethylglycine dehydrogenase 7.80E- Q9DBT9 mitochondrial GN = Dmgdh PE = 1 SV 1.36 03 = 1 Methyltransferase-like protein 7B 3.87E- Q9DD20 2.33 GN = Mettl7b PE = 1 SV = 2 02 Protein arginine N-methyltransferase 1 3.87E- Q9JIF0 2.62 GN = Prmt1 PE = 1 SV = 1 02 Carbohydrate metabolism Fructose-bisphosphate aldolase A GN = 2.59E- P05064 0.56 Aldoa PE = 1 SV = 2 02 6-phosphogluconate dehydrogenase 1.90E- Q9DCD0 0.64 decarboxylating GN = Pgd PE = 1 SV = 02 3 Aldo-keto reductase family 1 member 3.44E- Q8VC28 0.68 C13 GN = Akr1c13 PE = 1 SV = 2 02 Mannose-6-phosphate isomerase 1.49E- Q924M7 0.71 GN = Mpi PE = 1 SV = 1 02 Phosphoglycerate kinase 1 GN = Pgk1 1.31E- P09411 0.77 PE = 1 SV = 4 02 Phosphoglycerate mutase 1 GN = 5.04E- Q9DBJ1 0.77 Pgam1 PE = 1 SV = 3 03 UDP-glucuronosyltransferase 1-6 4.02E- Q64435 1.35 GN = Ugt1a6 PE = 1 SV = 1 02 Glycerol-3-phosphate dehydrogenase 4.87E- Q64521 mitochondrial GN = Gpd2 PE = 1 SV = 1.49 03 2 Urea cycle 4.35E- Q61176 Arginase-1 GN = Arg1 PE = 1 SV = 1 1.16 02 Carbamoyl-phosphate synthase 3.81E- Q8C196 [ammonia] mitochondrial GN = Cps1 1.29 02 PE = 1 SV = 2 2.62E- P25688 Uricase GN = Uox PE = 1 SV = 2 1.70 03 Ornithine aminotransferase 4.76E- P29758 mitochondrial 2.00 02 GN = Oat PE = 1 SV = 1 TCA cycle Glutamate dehydrogenase 1 1.18E- P26443 mitochondrial GN = Glud1 PE = 1 SV = 1.23 02 1 Serine--pyruvate aminotransferase 4.10E- O35423 1.38 mitochondrial GN = Agxt PE = 1 SV = 04 3 Aspartate aminotransferase 3.48E- P05202 mitochondrial GN = Got2 PE = 1 SV = 1.39 02 1 Succinate-semialdehyde 2.18E- Q8BWF0 dehydrogenase mitochondrial GN = 1.50 03 Aldh5a1 PE = 1 SV = 1 Succinate-CoA ligase (GDP-forming) 1.52E- Q9Z2I8 subunit beta mitochondrial GN = 1.55 03 Suclg2 PE = 1 SV = 3 Glutaminase liver isoform 1.23E- Q571F8 mitochondrial 1.59 02 GN = Gls2 PE = 1 SV = 2 Succinate-CoA ligase (ADP-forming) 5.11E- Q9Z2I9 subunit beta mitochondrial GN = 1.87 03 Sucla2 PE = 1 SV = 2 OxPhos & antioxidant response 4.84E- P24270 Catalase GN = Cat PE = 1 SV = 4 1.28 03 NADH-cytochrome b5 reductase 3 4.08E- Q9DCN2 1.32 GN = Cyb5r3 PE = 1 SV = 3 03 2.41E- P67778 Prohibitin GN = Phb PE = 1 SV = 1 1.35 02 ATP synthase subunit alpha 4.41E- Q03265 mitochondrial GN = Atp5a1 PE = 1 SV 1.38 03 = 1 ATP synthase subunit beta 1.68E- P56480 mitochondrial GN = Atp5b PE = 1 SV = 1.39 03 2 NADH-ubiquinone oxidoreductase 75 4.28E- Q91VD9 1.41 kDa subunit mitochondrial GN = 03 Ndufs1 PE = 1 SV = 2 Cytochrome b-c1 complex subunit 2 6.36E- Q9DB77 mitochondrial GN = Uqcrc2 PE = 1 SV 1.41 03 = 1 Electron transfer flavoprotein- ubiquinone oxidoreductase 3.06E- Q921G7 1.43 mitochondrial GN = Etfdh PE = 1 SV = 02 1 Glutathione S-transferase kappa 1 3.31E- Q9DCM2 1.43 GN = Gstk1 PE = 1 SV = 3 02 2.88E- O35129 Prohibitin-2 GN = Phb2 PE = 1 SV = 1 1.45 02 Cytochrome P450 2E1 GN = Cyp2e1 PE 2.20E- Q05421 1.51 = 1 SV = 1 02 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 1.26E- Q9DC69 1.54 mitochondrial 02 GN = Ndufa9 PE = 1 SV = 2 Cytochrome P450 2F2 GN = Cyp2f2 PE 1.13E- P33267 1.57 = 1 SV = 1 02 NADH dehydrogenase [ubiquinone] 1.92E- Q91YT0 flavoprotein 1 mitochondrial GN = 1.58 03 Ndufv1 PE = 1 SV = 1 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11 4.61E- O09111 1.89 mitochondrial GN = Ndufb11 PE = 1 02 SV = 2 Cytochrome P450 2A5 GN = Cyp2a5 PE 9.73E- P20852 4.29 = 2 SV = 1 03 .