SAN TA C RUZ BI OTEC HNOL OG Y, INC . DDX47 (2255C2): sc-81076 BACKGROUND APPLICATIONS DDX47 (Probable ATP-dependent RNA helicase DDX47) is a 455 amino acid DDX47 (2255C2) is recommended for detection of DDX47 of human origin by protein encoded by the human gene DDX47. DDX47 belongs to the DEAD-box Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000) and helicase family (DDX47/RRP3 subfamily) and contains one helicase ATP-bind - immunoprecipitation [1–2 µg per 100–500 µg of total protein (1 ml of cell ing domain and one helicase C-terminal domain. DDX47 is believed to be a lysate)]. probable ATP-dependent RNA helicase. RNA helicases are highly conserved Suitable for use as control antibody for DDX47 siRNA (h): sc-96180, DDX47 enzymes that utilize the energy derived from NTP hydrolysis to modulate the shRNA Plasmid (h): sc-96180-SH and DDX47 shRNA (h) Lentiviral Particles: structure of RNA. RNA helicases participate in all biological processes that sc-96180-V. involve RNA, including transcription, splicing and translation. Molecular Weight of DDX47: 51 kDa. REFERENCES Positive Controls: DDX47 (h): 293T Lysate: sc-117224. 1. O’Day, C.L., Chavanikamannil, F. and Abelson, J. 1996. 18S rRNA process - ing requires the RNA helicase-like protein Rrp3. Nucleic Acids Res. 24: RECOMMENDED SECONDARY REAGENTS 3201-3207. To ensure optimal results, the following support (secondary) reagents are 2. Doorbar, J., Elston, R.C., Napthine, S., Raj, K., Medcalf, E., Jackson, D., recommended: 1) Western Blotting: use goat anti-mouse IgG-HRP: sc-2005 Coleman, N., Griffin, H.M., Masterson, P., Stacey, S., Mengistu, Y. and (dilution range: 1:2000-1:32,000) or Cruz Marker™ compatible goat anti- Dunlop, J. 2000. The E1E4 protein of human papillomavirus type 16 asso - mouse IgG-HRP: sc-2031 (dilution range: 1:2000-1:5000), Cruz Marker™ ciates with a putative RNA helicase through sequences in its C-terminus. Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: J. Virol. 74: 10081-10095. sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecip- itation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). 3. Regard, J.B., Scheek, S., Borbiev, T., Lanahan, A.A., Schneider, A., Demetriades, A.M., Hiemisch, H., Barnes, C.A., Verin, A.D. and Worley, DATA P.F. 2004. Verge: a novel vascular early response gene. J. Neurosci. 24: 4092-4103. AB 100 K – 4. Abdelhaleem, M. 2005. RNA helicases: regulators of differentiation. Clin. 80 K – 132 K 60 K – 90 K Biochem. 38: 499-503. 50 K – 40 K – 55 K 5. Lee, J.H., Rho, S.B. and Chun, T. 2005. GABAA receptor-associated protein < < DDX47 fusion DDX47 (GABARAP) induces apoptosis by interacting with DEAD (Asp-Glu-Ala- 30 K – protein 43 K Asp/His) box polypeptide 47 (DDX 47). Biotechnol. Lett. 27: 623-628. 20 K – 34 K 6. Nousiainen, M., Silljé, H.H., Sauer, G., Nigg, E.A. and Körner, R. 2006. Phosphoproteome analysis of the human mitotic spindle. Proc. Natl. Acad. DDX47 (2255C2): sc-81076 Western Blot analysis of DDX47 (2255C2): sc-81076. Western blot analysis of human recombinant DDX47 fusion protein. DDX47 expression in non-transfected: sc-117752 ( A) Sci. USA 103: 5391-5396. and human DDX47 transfected: sc-117224 ( B) 293T whole cell lysates. 7. Sekiguchi, T., Hayano, T., Yanagida, M., Takahashi, N. and Nishimoto, T. 2006. NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47. Nucleic Acids Res. 34: 4593-4608. RESEARCH USE For research use only, not for use in diagnostic procedures. CHROMOSOMAL LOCATION Genetic locus: DDX47 (human) mapping to 12p13.1. PROTOCOLS See our web site at www.scbt.com or our catalog for detailed protocols and SOURCE support products. DDX47 (2255C2) is a mouse monoclonal antibody raised against a recombi - nant protein corresponding to an internal region of DDX47 of human origin. PRODUCT Each vial contains 100 µg IgG 1 in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% BSA. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.