Abstracts of papers presented at the 2015 meeting on THE UBIQUITIN FAMILY April 21–April 25, 2015 Abstracts of papers presented at the 2015 meeting on THE UBIQUITIN FAMILY April 21–April 25, 2015 Arranged by Ron Hay, University of Dundee, UK Ron Kopito, Stanford University Cynthia Wolberger, Johns Hopkins School of Medicine This meeting was funded in part by Boston Biochem. Contributions from the following companies provide core support for the Cold Spring Harbor meetings program. Corporate Sponsors Agilent Technologies Bristol-Myers Squibb Company Genentech Life Technologies (part of Thermo Fisher Scientific) New England BioLabs Plant Corporate Associates Monsanto Company ____________________________________________________________ Front Cover: Image by Patrick Lombardi, Johns Hopkins Medical Institute. THE UBIQUITIN FAMILY Tuesday, April 21 – Saturday, April 25, 2015 Tuesday 7:30 pm Keynote Speaker 1 Control of Cellular Functions by Ubiquitins and UBLs Wednesday 9:00 am 2 Quality Control Wednesday 2:00 pm 3 Poster Session I Wednesday 4:30 pm Wine and Cheese Party* Wednesday 7:30 pm 4 Control of E3 Ligase Activity Thursday 9:00 am 5 Regulation by Ubiquitin and SUMO Thursday 2:00 pm 6 Poster Session II Thursday 7:30 pm 7 Mechanism of Action of E3 Ligases Friday 9:00 am 8 Removal of Ubiquitin and UBLs Friday 2:00 pm Keynote Speaker 9 Autophagy Friday 6:00 pm Banquet Saturday 9:00 am 10 Proteasome * Airslie Lawn, weather permitting Mealtimes at Blackford Hall are as follows: Breakfast 7:30 am-9:00 am Lunch 11:30 am-1:30 pm Dinner 5:30 pm-7:00 pm Bar is open from 5:00 pm until late Abstracts are the responsibility of the author(s) and publication of an abstract does not imply endorsement by Cold Spring Harbor Laboratory of the studies reported in the abstract. These abstracts should not be cited in bibliographies. Material herein should be treated as personal communications and should be cited as such only with the consent of the author. Please note that ANY photography or video/audio recording of oral presentations or individual posters is strictly prohibited except with the advance permission of the author(s), the organizers, and Cold Spring Harbor Laboratory. Printed on 100% recycled paper. PROGRAM TUESDAY, April 21—7:30 PM SESSION 1 CONTROL OF CELLULAR FUNCTIONS BY UBIQUITIN AND UBLs Chairpersons: D. Barford, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom D. Komander, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom KEYNOTE SPEAKER Brenda Schulman St. Jude Children’s Research Hospital Implications for mechanisms of protein ubiquitination from a high resolution structure of the APC/C Leifu Chang, Ziguo Zhang, Jing Yang, Stephen H. McLaughlin, David Barford. Presenter affiliation: MRC Laboratory of Molecular Biology, Cambridge, United Kingdom. 1 Degradation of Ndd1 by APC/CCdh1 generates a feed forward loop that times mitotic protein accumulation Michael Brandeis, Drora Zenvirth, Mor Nitzan, Julia Sajman. Presenter affiliation: The Hebrew University of Jerusalem, Jerusalem, Israel. 2 Functional analysis of transcriptional regulation by sumoylation in yeast John Babu, Veroni S. Sri Theivakadacham, Justin M. Burgener, Akhi Akhter, Emanuel Rosonina. Presenter affiliation: York University, Toronto, Canada. 3 Ubiquitin receptor protein UBASH3B mediates a switch-like mechanism of Aurora B localization to microtubules Ksenia Krupina, Charlotte Kleiss, Kay Hofmann, Olivier Poch, Laurent Brino, Izabela Sumara. Presenter affiliation: IGBMC, Illkirch, France. 4 v WEDNESDAY, April 22—9:00 AM SESSION 2 QUALITY CONTROL Chairpersons: A. Bertolotti, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom W. Harper, Harvard Medical School, Boston, Massachusetts Coping with protein quality control failure Anne Bertolotti. Presenter affiliation: MRC LMB, Cambridge, United Kingdom. 5 Inhibition of LUBAC-mediated linear ubiquitination by a virulence factor of Aspergillus, gliotoxin Hiroki Sakamoto, Takayoshi Okabe, Tetsuo Nagano, Kazuhiro Iwai. Presenter affiliation: Graduate School of Medicine, Kyoto University, Kyoto, Japan. 6 USP30 and Parkin homeostatically regulate atypical ubiquitin chains on mitochondria Christian N. Cunningham, Joshua M. Baughman, Lilian Phu, Joy S. Tea, Christine Yu, Mary Coons, Donald S. Kirkpatrick, Baris Bingol, Jacob E. Corn. Presenter affiliation: Genentech, Inc., South San Francisco, California. 7 Rsp5 and Ubp2 jointly target cytosolic misfolded proteins for proteasome degradation upon heat denaturation Nancy N. Fang, Thibault Mayor. Presenter affiliation: University of British Columbia, Vancouver, Canada. 8 Misfolded mitochondrial protein degradation by the ubiquitin- proteasome system Meredith B. Metzger, Mitchell Dunklebarger, Allan M. Weissman. Presenter affiliation: National Cancer Institute, Frederick, Maryland. 9 CRL2 ubiquitin ligase eliminates truncated selenoproteins from ambiguous UGA/Sec translation Hsiu-Chuan Lin, Hsueh-Chi Sherry Yen. Presenter affiliation: Academia Sinica, Taipei, Taiwan; National Taiwan University, Taipei, Taiwan. 10 vi The E3 ligase Ubr3 regulates Usher syndrome proteins in Drosophila and zebrafish auditory organs Tongchao Li, Bernardo Blanco-Sánchez, Nikolaos Giagtzoglou, Shinya Yamamoto, Manish Jaiswal, Sonal Nagarkar Jaiswal, Daniel F. Eberl, Dorothea Godt, Monte Westerfield, Andrew K. Groves, Hugo J. Bellen. Presenter affiliation: Baylor College of Medicine, Houston, Texas; Texas Children’s Hospital, Houston, Texas. 11 Regulation of ubiquitin metabolism by a direct phosphorylation mechanism Sora Lee, Kristin E. Jernigan, Aaron C. Ehlinger, Jessica M. Tumolo, Walter J. Chazin, Scott D. Emr, Jason A. MacGurn. Presenter affiliation: Vanderbilt University, Nashville, Tennessee. 12 The Ubiquitin Code v2.0—Phospho-ubiquitin David Komander. Presenter affiliation: MRC Laboratory of Molecular Biology, Cambridge, United Kingdom. 13 WEDNESDAY, April 22—2:00 PM SESSION 3 POSTER SESSION I An inhibitor of ubiquitin conjugation system Heeseon An, Alexander V. Statsyuk. Presenter affiliation: Northwestern University, Evanston, Illinois. 14 UCHL3 hydrolysis of ubiquitin chains is regulated by allostery as determined by NMR Larry J. Anderson, Robert G. Guenette, Kishan M. Patel, Marco Tonelli, Eric R. Strieter. Presenter affiliation: University of Wisconsin - Madison, Madison, Wisconsin. 15 Development of a drug discovery platform targeting SUMO specific proteases Nadia Arnaudo, Yaara Ofir-Rosenfeld, Louise Jones, Jeanine Harrigan, Lisa Smith, Helen Robinson, Mark Kemp, Xavier Jacq. Presenter affiliation: MISSION Therapeutics, Cambridge, United Kingdom. 16 vii Proteasome inhibitors increase the growth inhibition of drugs targeting DNA topoisomerase II (TOP2) Ka Cheong Lee, Rebecca L. Bramley, Ian G. Cowell, Caroline A. Austin. Presenter affiliation: Newcastle University, Newcastle upon Tyne, United Kingdom. 17 Allosteric cross talk between the deubiquitinase Ubp6 and the 26S proteasome Charlene Bashore, Corey Dambacher, Mary Matyskiela, Gabriel Lander, Andreas Martin. Presenter affiliation: UC Berkeley, Berkeley, California. 18 Conjugation of the ubiquitin activating enzyme UBE1 with the ubiquitin-like modifier FAT10 targets it for proteasomal degradation Johanna Bialas, Marcus Groettrup, Annette Aichem. Presenter affiliation: University of Konstanz, Konstanz, Germany; Biotechnology Institute Thurgau, Kreuzlingen, Switzerland. 19 Molluscum contagiosum virus inhibits NF-κB activation by inhibiting ubiquitination of NEMO Sunetra Biswas, Joanna L. Shisler. Presenter affiliation: University of Illinois, Urbana-Champaign, Urbana, Illinois. 20 Ubiquitination of HOIP carboxyl-terminus negatively regulates linear ubiquitination-mediated immune signaling James W. Bowman, Mary A. Rodgers, Mude Shi, Rina Amatya, Bruce Hostager, Kazuhiro Iwai, Jae U. Jung. Presenter affiliation: University of Southern California, Los Angeles, California. 21 The BMI1/RING1A E3 ubiquitin ligase inhibitor PRT4165 increases the growth inhibition of drugs targeting DNA topoisomerase II (TOP2) Rebecca L. Bramley, Ka Cheong Lee, Ian G. Cowell, Caroline A. Austin. Presenter affiliation: Newcastle University, Newcastle-upon-Tyne, United Kingdom. 22 viii Cezanne (OTUD7B) regulates HIF-1α homeostasis in a proteasome-independent manner Anja Bremm, Sonia Moniz, Julia Mader, Sonia Rocha, David Komander. Presenter affiliation: Goethe University Frankfurt, Frankfurt am Main, Germany. 23 Cdc48 complexes required for multiple steps of SREBP cleavage in S. pombe Risa Burr, Sumana Raychaudhuri, Diedre Ribbens, Jiwon Hwang, Peter Espenshade. Presenter affiliation: Johns Hopkins University School of Medicine, Baltimore, Maryland. 24 Studying dimer-dependent Fbw7-substrate interactions: using an ALPHAscreen to quantitate degron cooperativity in full–length substrates Shlomo Handelli, Markus Welcker, Bruce E. Clurman. Presenter affiliation: Fred Hutchinson Cancer Research Center, Seattle, Washington. 25 Distinct activation of an E2 ubiquitin conjugating enzyme by its cognate E3 ligases Itamar Cohen, Yuval Reiss, Reuven Wiener, Tommer Ravid. Presenter affiliation: The Hebrew University of Jerusalem, Jerusalem, Israel. 26 Muscle denervation, as occur with aging, enhances proteolysis and causes wasting by a mechanism involving the ubiquitin ligase Trim32 and the calcium-dependent protease, Calpain 1 Inga Rudesky, Eitan Shimko, Shenhav Cohen. Presenter affiliation: Technion Institute of Technology, Haifa, Israel. 27 HOIP deficiency causes embryonic lethality by aberrant TNFR1- mediated endothelial cell death