PIBF1 Antibody Cat. No.: 7237 PIBF1 Antibody Immunohistochemistry of PBIF in human spleen tissue Immunofluorescence of PBIF in human spleen tissue with with PBIF antibody at 5 μg/ml. PBIF antibody at 20 μg/ml. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat Rabbit polyclonal PIBF1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human PIBF1. IMMUNOGEN: The immunogen is located within amino acids 610 - 660 of PIBF1. TESTED APPLICATIONS: ELISA, IF, IHC-P, WB PIBF1 antibody can be used for detection of PIBF1 by Western blot at 1 - 2 μg/mL. APPLICATIONS: Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. October 1, 2021 1 https://www.prosci-inc.com/pibf1-antibody-7237.html SPECIFICITY: Multiple isoforms of PIBF1 exists as a result of alternative splicing event. POSITIVE CONTROL: 1) Cat. No. 1309 - Human Placenta Tissue Lysate 2) Cat. No. 10-901 - Human Spleen Tissue Slide PREDICTED MOLECULAR 83 kDa WEIGHT: Properties PURIFICATION: PIBF1 Antibody is affinity chromatography purified via peptide column. CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PIBF1 Antibody is supplied in PBS containing 0.02% sodium azide. CONCENTRATION: 1 mg/mL PIBF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. Additional Info OFFICIAL SYMBOL: PIBF1 ALTERNATE NAMES: PIBF1 Antibody: PIBF, CEP90, C13orf24, PIBF, Progesterone-induced-blocking factor 1 ACCESSION NO.: NP_006337 PROTEIN GI NO.: 5576958 GENE ID: 10464 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References PIBF1 Antibody: PIBF1 is synthesized during pregnancy in response to progesterone by T lymphocytes. PIBF1 inhibits arachidonic acid release, controls NK activity, and modifies the cytokine balance exerting an anti-abortive effect. It contains a leucine zipper motif, a basic zipper sequence, a PEST sequence, a nuclear localization signal, an ER membrane BACKGROUND: retention signal and N-glycosylation and phosphorylation sites. PIBF1 is significantly higher in healthy pregnant women than in women at risk for premature pregnancy termination. Full-length PIBF1 is associated with the nucleus and functions as a transcription factor, whereas secretion of shorter forms which may act as cytokines is induced by activation of the cell. October 1, 2021 2 https://www.prosci-inc.com/pibf1-antibody-7237.html 1) Laskarin G, Tokmadzic VS, Strbo N, et al. Progesterone induced blocking factor (PIBF) REFERENCES: mediates progesterone induced suppression of decidual lymphocyte cytotoxicity. Am. J. Reprod. Immunol. 2002; 48:201-9. 2) Lachmann M, Gelbmann D, Kalman E, et al. PIBF (progesterone induced blocking factor) is overexpressed in highly proliferating cells and associated with the centrosome. Int. J. Cancer. 2004; 112:51-60 3) Polgar B, Kispal G, Lachmann M, et al. Molecular cloning and immunologic characterization of a novel cDNA coding for progesterone-induced blocking factor. J. Immunol. 2003; 171:5956-63. 4) Raghupathy R, Al-Mutawa E, Al-Azemi M, et al. Progesterone-induced blocking factor (PIBF) modulates cytokine production by lymphocytes from women with recurrent miscarriage or preterm delivery. J. Reprod. Immunol. 2009; 80:91-9. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. October 1, 2021 3 https://www.prosci-inc.com/pibf1-antibody-7237.html.