KPNA1 Antibody Cat. No.: 5981 Western blot analysis of KPNA1 in Hela cell lysate with KPNA1 antibody at 1μg/mL. Immunocytochemistry of KPNA1 in HeLa cells with KPNA1 antibody at 2.5 μg/mL. Immunofluorescence of KPNA1 in K562 cells with KPNA1 antibody at 20 μg/mL. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%) KPNA1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human KPNA1. IMMUNOGEN: The immunogen is located within amino acids 30 - 80 of KPNA1. TESTED APPLICATIONS: ELISA, ICC, IF, WB September 30, 2021 1 https://www.prosci-inc.com/kpna1-antibody-5981.html KPNA1 antibody can be used for detection of KPNA1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS: Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. POSITIVE CONTROL: 1) Cat. No. 1201 - HeLa Cell Lysate 2) Cat. No. 17-001 - HeLa Cell Slide 3) Cat. No. 17-004 - K-562 Cell Slide Properties PURIFICATION: KPNA1 Antibody is affinity chromatography purified via peptide column. CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: KPNA1 Antibody is supplied in PBS containing 0.02% sodium azide. CONCENTRATION: 1 mg/mL KPNA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: KPNA1 KPNA1 Antibody: RCH2, SRP1, IPOA5, NPI-1, RCH2, Importin subunit alpha-5, Karyopherin ALTERNATE NAMES: subunit alpha-1 ACCESSION NO.: NP_002255 PROTEIN GI NO.: 222144293 GENE ID: 3836 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References September 30, 2021 2 https://www.prosci-inc.com/kpna1-antibody-5981.html KPNA1 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA1, also known as BACKGROUND: importin alpha 5, is the alpha subunit of karyopherin, which forms a complex with importin subunit beta-1 and functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. It is ubiquitously expressed and polyubiquitinated in the presence of RAG1. KPNA1 interacts with various virus nucleoproteins, including those of Ebola and influenza. 1) Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. REFERENCES: Gene Expr.1997; 7:61-72. 2) Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49. 3) Simkus C, Makiya M and Jones JM. Karyopherin alpha 1 is a putative substrate of the RAG1 ubiquitin ligase. Mol. Immunol.2009; 46:1319-25. 4) Reid SP, Valmas C, and Martinez O. Ebola virus VP24 proteins inhibit the interaction of NPI-1 subfamily karyopherin alpha proteins with activated STAT1. J. Virol.2007; 81:13469-77. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 30, 2021 3 https://www.prosci-inc.com/kpna1-antibody-5981.html.