10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 CD300f Polyclonal Antibody Catalog # AP73658 Specification CD300f Polyclonal Antibody - Product Information Application WB Primary Accession Q8TDQ1 Reactivity Human Host Rabbit Clonality Polyclonal CD300f Polyclonal Antibody - Additional Information Gene ID 146722 Other Names CD300LF; CD300F; CLM1; IGSF13; IREM1; NKIR; CMRF35-like molecule 1; CLM-1; CD300 antigen-like family member F; Immune receptor expressed on myeloid cells 1; IREM-1; Immunoglobulin superfamily member 13; IgSF13; NK inhibitory receptor; CD300f Dilution WB~~Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. Format Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage Conditions -20℃ CD300f Polyclonal Antibody - Protein Information Name CD300LF Synonyms CD300F, CLM1, IGSF13, IREM1, NKIR Function Acts as an inhibitory receptor for myeloid cells and mast cells (PubMed:<a href="http ://www.uniprot.org/citations/15549731" target="_blank">15549731</a>). CD300f Polyclonal Antibody - Background Positively regulates the phagocytosis of Page 1/2 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 apoptotic cells (efferocytosis) via Acts as an inhibitory receptor for myeloid phosphatidylserine (PS) recognition; cells and mast cells (PubMed:15549731). recognizes and binds PS as a ligand which is Positively regulates the phagocytosis of expressed on the surface of apoptotic cells. apoptotic cells (efferocytosis) via Plays an important role in the maintenance phosphatidylserine (PS) recognition; recognizes of immune homeostasis, by promoting and binds PS as a ligand which is expressed on macrophage-mediated efferocytosis and by the surface of apoptotic cells. Plays an inhibiting dendritic cell-mediated important role in the maintenance of immune efferocytosis (By similarity). Negatively homeostasis, by promoting regulates Fc epsilon receptor-dependent macrophage-mediated efferocytosis and by mast cell activation and allergic responses inhibiting dendritic cell-mediated efferocytosis via binding to ceramide and sphingomyelin (By similarity). Negatively regulates Fc epsilon which act as ligands (PubMed:<a href="htt receptor-dependent mast cell activation and p://www.uniprot.org/citations/24035150" allergic responses via binding to ceramide and target="_blank">24035150</a>). May act as a coreceptor for interleukin 4 (IL-4). sphingomyelin which act as ligands Associates with and regulates IL-4 receptor (PubMed:24035150). May act as a coreceptor alpha-mediated responses by augmenting for interleukin 4 (IL-4). Associates with and IL-4- and IL-13-induced signaling (By regulates IL-4 receptor alpha-mediated similarity). Negatively regulates the Toll-like responses by augmenting IL-4- and receptor (TLR) signaling mediated by IL-13-induced signaling (By similarity). MYD88 and TRIF through activation of Negatively regulates the Toll-like receptor PTPN6/SHP-1 and PTPN11/SHP-2 (TLR) signaling mediated by MYD88 and TRIF (PubMed:<a href="http://www.uniprot.org/c through activation of PTPN6/SHP-1 and itations/22043923" PTPN11/SHP-2 (PubMed:22043923). Inhibits target="_blank">22043923</a>). Inhibits osteoclast formation. Induces macrophage cell osteoclast formation. Induces macrophage death upon engagement (By similarity). cell death upon engagement (By similarity). Cellular Location Cell membrane; Single-pass type I membrane protein Tissue Location Highly expressed in spleen, peripheral blood leukocyte and monocyte, and lung. Weakly expressed in thymus, heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine or colon. Expressed selectively in monocytes and monocyte-related cells. CD300f Polyclonal Antibody - Protocols Provided below are standard protocols that you may find useful for product applications. • Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture Page 2/2 Powered by TCPDF (www.tcpdf.org).