Curriculum Vitae JANET KAZUKO YAMAMOTO, Ph.D. PRESENT POSITION: RESIDENCE: Professor 4309 SW 77th Street Department of Infectious Diseases & Pathology (ID&P) Gainesville, Florida 32608 College of Veterinary Medicine Phone: (352) 371-9219 University of Florida, PO Box 110880 Fax: (352) 337-0444 Gainesville, FL 32611-0880 Phone: (352) 294-4145 Fax: (352) 392-7128 E-mail: [email protected] EMPLOYMENT HISTORY: 1979-1981 James W. McLaughlin Predoctoral Fellow in Infection and Immunity, Department of Microbiology, The University of Texas Medical Branch, Galveston, (Supervisor, Dr. Howard M. Johnson). Characterized the xenogeneic responses observed during the interaction of human peripheral blood lymphocytes and mouse spleen cells. Identified human natural killer cell activity towards mouse immunocytes, nonspecific soluble helper activity of human T cells towards mouse antibody response, and suppressor cell activity of mitogen and alloantigen-stimulated human T cells towards mouse antibody response. 1981-1982 NIH PHS Postdoctoral Fellow in the laboratory of Drs. Richard W. Dutton and Susan L. Swain, Department of Biology, University of California, San Diego. Evaluated the role of lymphokines (interleukin-2 and interferon-γ) in the developing primary and secondary antibody responses in mice. 1982-1983 Research Associate Scientist in the laboratory of Drs. Robert A. Good and N.K. Day, Oklahoma Medical Research Foundation, Oklahoma City. Analyzed the immunological status of feline leukemia virus (FeLV)-positive cats that were undergoing ex vivo immunoabsorption therapy with protein A column. 1983-1985 Postgraduate Researcher IV in the Department of Medicine, School of Veterinary Medicine, University of California, Davis. Produced and purified feline interferons (α, ß, and γ) and characterized their effect against FeLV. Received New Investigator Research Award (1984-1987) from NCI, NIH to conduct research on the role of lymphokines in FeLV-induced immunosuppression and leukemia. Recruited concurrently in the fall of 1984 by Dr. Murray B. Gardner to establish the UCD AIDS Laboratory and to supervise the large-scale production and purification of HIV isolates for the Department of Pathology, School of Medicine, UCD. 1985 -1991 Assistant Research Immunologist I, II, III, IV, in the Department of Medicine, School of Veterinary Medicine, University of California, Davis. Served as a member of Center Janet K. Yamamoto for AIDS Research (CFAR) at UCD and directed a research program on characterization of FIV immunopathogenesis, development of effective FIV vaccines, and identifying innovative immunotherapeutic approaches against animal and human AIDS. 1991-1993 Adjunct Associate Professor I (starting July 1, 1992) / Associate Research Immunologist I (1991 to June 30, 1992) in the Department of Medicine, School of Veterinary Medicine, University of California, Davis. Served as a member of CFAR at UCD and directed a research program identifying immune mechanism(s) of FIV vaccine protection and determining the immunopathological effects of B-cell hyperactivities in FIV infected cats. 1993-present. Associate Professor (tenured 1997) / Professor (promoted 2001) in the Department of Infectious Diseases & Pathology*, College of Veterinary Medicine, University of Florida, Gainesville, Florida. Responsible for teaching and coordinating veterinary immunology course (1993-2005); research director of the FIV vaccine and immunotherapy program; founder and executive member of Comparative Clinical Immunology Program. Affiliate faculty of Microbiology and Cell Sciences, Institute of Food and Agricultural Sciences (IFAS), University of Florida (1993-pres.). * Please note that the Department of Infectious Diseases & Pathology was initially called the Department of Pathobiology upon fusion of two College of Veterinary Medicine (CVM) departments, the Department of Comparative & Experimental Pathology and the Department of Infectious Diseases, and to its current name in December of 2005. EDUCATION: 1976 B.A. in Biological Sciences, University of California, Davis, California. 1981 Ph.D. in Microbiology (Immunology major, Virology minor), The University of Texas Medical Branch, Galveston, Texas. MEMBERSHIP IN SCIENTIFIC ORGANIZATIONS: American Association of Immunologists American Society for Microbiology American Association for the Advancement of Science New York Academy of Sciences International AIDS Society Clinical Immunology Society 2 Janet K. Yamamoto HONORS: 1979-1981 James W. McLaughlin Predoctoral Fellow in Infection and Immunity. 1984-1987 New Investigator Research Award, NCI, NIH. 1988 Rhone Merieux Workshop on Animal AIDS Models (Invited Speaker), Lyon, France. 1988 World Health Organization Conference on Animal Models of AIDS (Invited Speaker), Geneva, Switzerland. 1991 International Feline Immunology and Immunodeficiency Workshop (Invited Speaker), Glasgow, UK. 1992 Septieme Colloque Des Cent Gardes on Retroviruses of Human AIDS and Related Animal Diseases (Invited Speaker), Pasteur Institute, Marnes-la-Coquette, France. 1993 Annual Meeting by NIH, NCI, Laboratory of Tumor Cell Biology (Invited Speaker). 1995 XVIII Symposium of the International Association for Comparative Research on Leukemia and Related Diseases in Kyoto, Japan (Invited Speaker). 1996 Pfizer Animal Health Award for Research Excellence at the University of Florida, College of Veterinary Medicine (cited in JAVMA, 209:710). 1997 Plenary lecturer for Vaccine Section, 2nd Intl. Feline Immunol. Workshop, Davis, CA. 2002 The University of Florida Research Foundation Professors Award. 2002 Honorary Member of Upsilon Chapter of The Society of Phi Zeta (Honor Society of Veterinary Medicine). 2002 America’s Registry of Outstanding Professionals. 2003 Who’s Who in Veterinary Medicine Higher Education. 2003 Who’s Who in Science and Engineering. 2005 Pfizer Animal Health Award for Research Excellence at the University of Florida, College of Veterinary Medicine (received this award twice). 2006 Who’s Who in America. 2007 Faculty Achievement Recognition at the University of Florida. 2014 National Academy of Inventors (NIA) Fellow. 2015 Congressional Record – Extension of Remarks on 02/20/2015 on 2014 NAI 2015 Florida Inventors Hall of Fame. PROFESSIONAL ACCOMPLISHMENTS: BSL-3 Laboratories • Assisted in establishing the first UCD AIDS BSL-3 laboratory in 1984 as a joint venture for the Schools of Medicine and Veterinary Medicine; in charge of large-scale production of three HIV-1 strains for development of immunodiagnostics. This laboratory eventually became UCD AIDS Diagnostic Laboratory in 1986 which served as a major HIV-1 BSL-3 facility for the Center for AIDS Research (CFAR) grant awarded to UCD. • Served as the member of the NIH-funded CFAR at UCD (1985-1993) and pursued independent research program in retrovirology and immunology in the second AIDS BSL-3 laboratory. 3 Janet K. Yamamoto • Established and successfully operated numerous BSL-2+ and BSL-3 laboratories. Served as a consultant to the development of the second FDA-approved HIV immunoblot by Bio-Rad Laboratories in 1988. Directed and managed collaborative research projects on evaluating relative efficacies of therapeutic drugs and vaccine approaches against a number of feline and human viral infections. The work on HIV included evaluating in vitro drug efficacy and toxicity. Discoveries and Developments • Co-discovered, characterized, and co-patented the feline interferon-/ (U.S. Patent No. 4,861,720) (1986). Performed phase-I trials as a joint venture with a Japanese firm under UCD contract (1988-1990). Released feline interferon license to a Japanese biochemical corporation for therapeutic use in viral diseases of cats and dogs. Currently receiving royalty income. • Co-discovered, characterized, and co-patented the FIV (U.S. Patent Nos. 5,037,753; 5,118,602) (1986-1987). This virus which closely mimics human immunodeficiency virus (HIV) is useful in studying the pathogenesis of lentiviruses, in testing combinations of antiviral and immunomodulating drugs, and in evaluating vaccine approaches for AIDS. Developed, characterized, and patented IL-2-independent FIV producer cell lines to be used for developing FIV vaccine and diagnostics (U.S. Patent Application No. 5,275,813; 5,275,813; 5,510,106) (1990). • Developed, characterized, and patented multi-subtype FIV vaccines (U.S. Patent No. 6,447,993B1; European PCT: Patent No. 728750) (1993-2001) at the University of Florida. Our dual-subtype FIV vaccine, released by Fort Dodge Animal Health in 2002, is the first commercial AIDS lentivirus vaccine. Developed and patented feline IL-2-independent cell lines useful for large-scale FIV production (U.S. Patent No. 5,846,825) (1994-1997). Currently receiving royalty and licensing income for both FIV diagnostics and vaccines. Donated a portion of FIV vaccine income to develop the College of Veterinary Medicine’s Comparative Clinical Immunology Program which had a faculty membership of 17 (14 tenured) in 2000. In collaboration with Dr. Ben Dunn of College of Medicine, University of Florida in 1997-2001 on the development and use of FIV/HIV-1 protease inhibitors leading to joint patent application (US Patent US6,875,773B1). • Discovered, characterized, and patented evolutionarily conserved T-cell epitopes on FIV and HIV-1 p24 and reverse transcriptase as immunogen for HIV-1 vaccine for humans and second generation FIV vaccine for cats (Provisional Application No. 61/681,014; No. 61/684,592; 61/841,122). Experience as Instructor, Reviewer,