21.10.2014RTN4RL2 antibody (pAb) Rabbit Anti-Human Reticulon 4 receptor-like 2 (NgR2, NGRH1, NGRL3, Nogo -66 receptor -related protein) Instruction Manual Catalog Number PK-AB718-7145 Synonyms RTN4RL2 Antibody: Reticulon 4 receptor-like 2, NgR2, NGRH1, NGRL3, Nogo receptor-like 3, Nogo-66 receptor homolog 1, Nogo-66 receptor-related protein Description Reticulon 4 receptor-like 2 (RTN4RL2), also known as NgR2and NgRH1, is a 60 kDa glycosylphosphatidylinositol-anchored protein that is expressed primarily in the brain. It contains eight leucine-rich repeats that are flanked by cysteine-rich sequences at both the N- and C-termini. The membrane anchored RTN4RL2 can be solubilized through the action of phospholipase or an unidentified MTMMP to generate a 46 kDa soluble receptor. It has been suggested that RTN4RL2 may play a role in regulating axonal regeneration and plasticity in the adult central nervous system. Quantity 100 µg Source / Host Rabbit Immunogen Rabbit polyclonal RTN4RL2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RTN4RL2. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human Specificity Three alternatively spliced transcript variants have been observed. RTN4RL2 antibody is predicted to not cross-react with other Nogo receptor-like proteins. Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. RTN4RL2 antibody can be used for detection of RTN4RL2 by Western blot at 1 - 2 µg/mL. Images Available upon request. References Pignot V, Hein AE, Barske C, et al. Characterization of two novel proteins, NgRH1 and NgRH2, structurally and biochemically homologous to the Nogo-66 receptor. J. Neurochem. 2003; 85:717- 28. Venkatesh K, Chivatakam O, Lee H, et al. The Nogo-66 receptor homolog NgR2 is a sialic acid- dependent receptor selective for myelin-associated glycoprotein. J. Neurosci. 2005; 25:808-22. Images Available upon request. Related Products Cat. No. PK-AB718-7145 - RTN4RL2 Peptide Cat. No. PK-AB718-1463 - Rat Brain Tissue Lysate FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES. PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014 .