OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] AP51638PU-N Polyclonal Antibody to FBXW8 (Center) - Aff - Purified Alternate names: F-box and WD-40 domain-containing protein 8, F-box only protein 29, F-box/WD repeat-containing protein 8, FBW6, FBW8, FBX29, FBXO29, FBXW6 Quantity: 0.4 ml Concentration: lot specific Background: This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains a WD-40 domain, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Uniprot ID: Q8N3Y1 NCBI: NP_036306 GeneID: 26259 Host / Isotype: Rabbit / Ig Immunogen: KLH conjugated synthetic peptide between 273-303 amino acids from the Central region of human FBXW8 Format: State: Liquid purified Ig fraction Purification: Affinity chromatography on Protein A Buffer System: PBS Preservatives: 0.09% (W/V) sodium azide Applications: ELISA: 1/1000. Western Blot: 1/100 - 1/500. Immunohistochemistry on paraffin sections: 1/50 - 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Molecular Weight: 67394 Da Specificity: This antibody reacts to FBXW8. Species Reactivity: Tested: Human and Mouse. Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MP/20130911 1 / 3 AP51638PU-N: Polyclonal Antibody to FBXW8 (Center) - Aff - Purified General Readings: 1. Tsutsumi, T., et al. Mol. Cell. Biol. 28(2):743-751(2008) 2. Koch, H.B., et al. Cell Cycle 6(2):205-217(2007) 3. Okabe, H., et al. PLoS ONE 1, E128 (2006) 4. Watanabe, N., et al. Proc. Natl. Acad. Sci. U.S.A. 101(13):4419-4424(2004) 5. Dias, D.C., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16601-16606(2002) Pictures: FBXW8 Antibody (Center) (Cat. #AP51638PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXW8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. FBXW8 Antibody (Center) (Cat. #AP51638PU-N) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the FBXW8 antibody detected the FBXW8 protein (arrow). FBXW8 Antibody (Center) (Cat. #AP51638PU-N) western blot analysis in Hela cell line lysates (35µg/lane).This demonstrates the FBXW8 antibody detected the FBXW8 protein (arrow). For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MP/20130911 2 / 3 AP51638PU-N: Polyclonal Antibody to FBXW8 (Center) - Aff - Purified FBXW8 Antibody (Center) (Cat. #AP51638PU-N) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti- rabbit secondary antibodies were used for the analysis. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MP/20130911 3 / 3 Powered by TCPDF (www.tcpdf.org).