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Application Data USA 101 : 9205 - 9210 . * Thornton et al. , 2000 , Nature Structural Biology , structural genomic ( 60 ) Provisional application No . 61/ 866 , 067, filed on Aug . supplement , Nov. 2000 : 991 - 994 . * 15 , 2013 , provisional application No . 61/ 864, 671 , Brachypodium distachyon protoporphyrinogen oxidase with UniProt filed on Aug. 12 , 2013 , provisional application No . accession No. I1IZ42 , published on Jun . 13 , 2012 . * 61/ 864, 672 , filed on Aug. 12 , 2013 . Koch et al. , 2004 , EMBO J . 23 : 1720 - 1728 . * (Continued ) (51 ) Int. Ci. C12N 15 / 82 ( 2006 . 01 ) Primary Examiner — Bratislav Stankovic AOIN 37 / 48 ( 2006 .01 ) (74 ) Attorney, Agent, or Firm — Marshall , Gerstein & AOIN 43 /54 ( 2006 .01 ) Borun LLP A01N 43 /653 ( 2006 .01 ) (57 ) ABSTRACT A01N 43 /84 ( 2006 . 01 ) The invention refers to plants comprising wild -type or AOIN 57/ 16 ( 2006 . 01 ) mutated Alopecurus PPO enzymes, and methods of obtain C12N 9 / 02 ( 2006 .01 ) ing such plants . The present invention further refers to a (52 ) U .S . CI. method for controlling weeds at a plant cultivation site , the CPC .. .. .. C12N 15 /8274 ( 2013 .01 ) ; A01N 37 / 48 method comprising the steps of providing , at said site , a ( 2013 .01 ) ; AOIN 43 / 54 (2013 .01 ) ; AOIN plant that comprises at least one nucleic acid comprising a 43 /653 (2013 .01 ) ; AOIN 43 /84 ( 2013 .01 ) ; nucleotide sequence encoding a wild - type or a mutated AOIN 57/ 16 ( 2013 .01 ) ; C12N 9 /001 Alopecurus PPO enzyme which is resistant or tolerant to a ( 2013 .01 ) ; C12N 9 /0071 ( 2013 .01 ) ; C12Y PPO - inhibiting herbicide by applying to said site an effec 103 /03004 ( 2013 .01 ) tive amount of said herbicide . (58 ) Field of Classification Search None 7 Claims, 6 Drawing Sheets See application file for complete search history . Specification includes a Sequence Listing . US 10 ,392 ,630 B2 Page 2 (56 ) References Cited WO WO - 2015 /022636 A2 2 / 2015 WO W O - 2015 /022639 A2 2 / 2015 U . S . 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