Curriculum Vitae PERSONAL INFORMATION First Name: Lisandro Horacio Surname: Otero Nationality: Argentinean Date of Birth: 03/18/1980 Working Address: Leloir Institute Foundation, Av. Patricias Argentinas 435, Buenos Aires, Argentina, PC C1405BWE. Telephone: +054-11-5238-7500 ext. 2552 Fax: +054-11-5238-7501 E-mail: [email protected] Current Position/Job: Assistant Researcher CONICET at Leloir Institute Foundation. Main coordinator of Argentina-Uruguay Block Allocation Group at ALBA synchrotron (beamline XALOC). EDUCATION Grade: Microbiologist (2005) Post-grade: PhD in Biological Sciences (2010) TEACHING ACTIVITIES Grade:- First assistant of the Area Biological Chemistry I and II. UNRC-Argentina. (2006-2011). Post-grade: - Assistant Professor. Course: From gene to crystallization of an enzyme to know its function”. UNRC- Argentina. (2010) ADMINISTRATIVE WORK - Committee member of ARGENTINE BIOLOGY OLYMPIAD. UNRC-Argentina. (2004-2005). - Committee member of INTERNATIONAL BIOLOGY OLYMPIAD. UNRC-Argentina. (2006). RESEARCH ACTIVITIES Research projects -11 projects (last years are shown) - Title: “Novel oxadiazols for the treatment of drug-resistant Gram-positive bacteria” Director: PhD Mayland Chang (coordinator) and PhD Juan Hermoso (european PI). NIH/NIAID-1R01 AI090818. (2010-2015). IQFR. Spain. - Title: “Bioinformatic platform for the discovery of new drugs based on the receptor-BIPEDD2 structure”. Director: PhD Federico Gago and PhD Juan Hermoso. S2010/BMD-2457. Council of Madrid (2012-2013). IQFR. Spain. - Title: “Structural basis on mechanisms of pathogenicity and antibiotic resistance in bacteria”. Director: PhD Juan Hermoso. (I-LINK0319). CSIC. (2012-2013). IQFR. Spain. - Title: “Structural biology of proteins from the bacterial cell wall: Implications on host-pathogen interactions”. Director: PhD Juan Hermoso. (BFU2011-25326). MCI. (2012-2013). IQFR. Spain. Fellowships Grade: - Student assistant research fellow SECyT. UNRC-Argentina. (2002-2003). - Student assistant research fellow SECyT. UNRC-Argentina. (2005-2006). Post-grade (nationals): -Postgraduate Fellow CONICET-MCyT. UNRC-Argentina (2006-2011). Post-grade (internationals): - Postgraduate Fellow “Programa de Formación Permanente” Fundación Carolina-España. IQFR-Spain. (2010). Post-doctoral (nationals): - Postdoctoral Fellow CONICET. FIL-Argentina. (2013-2014). Post-doctoral (internationals): - Postdoctoral contract by public concourse CSIC-Spain / NIH-EEUU. IQFR-Spain. (2011-2013). Presentations at conferences and seminars -24 presentations, 17 nationals and 7 internationals (last years are shown) - How PchP structural domains cooperate in the phosphorylcholine hydrolysis. Infantes, L; Otero, LH; Boetsch, C; Beassoni, PR; Lisa, AT; Domenech, CE; Albert, A. I meeting of the RSEQ Chemical Biology Group. Real Sociedad Española de Química. Santiago de Compostela. Spain. From 8th to 9th of March 2012. Oral communication. - 3D Structure and conformational changes in DNA topoisomerase of Streptococcus pneumonia. Otero, LH; García, MT; de la Campa, A; Hermoso, JA. XI European Meeting on the Molecular Biology of the Pneumococcus (EuroPneumo 2013). Madrid. Spain. From 28th to 31st of May 2013. Poster. - Allosteric inhibition of Staphylococcus aureus PBP2a by a quinazolinone antibacterial. Bouley, R; Otero, LH; Kumarasiri, M; Hermoso, JA; Chang, M; Mobashery, S. Chemistry-Biology-Interface Career Development Conference University of Illinois-Urbana, Champagne. Indiana. USA. 5th of June 2013. Oral communication. - Investigating the mechanism of action and efficacy of a quinazolinone anti-MRSA antibacterial. Bouley, R; Otero, LH; Kumarasiri, M; Song, W; Peng, Z; Shroeder,V; Antunes, N; Lastochkin, L; Suckow, M; Hermoso, JA; Chang, M; Mobashery, S. Medicinal Chemistry Gordon Research Conference. Colby-Sawyer College, New London, NH. USA. From 4th to 9th of August 2013. Poster. - 3D Structure and conformational changes in DNA topoisomerase of Streptococcus pneumonia. Otero, LH; García, MT; de la Campa, A; Hermoso, JA. 1st Latin-American Meeting on Crystallography- 9th Argentinean Annual Meeting on Crystallography. Córdoba. Argentina. From 29th to 31st of October 2013. Poster. - Crystal structure of BA42, a protein from the Antarctic bacterium Bizionia argentinensis comprised of a stand-alone TPM domain. Otero, LH; Klinke, S; Aran, M; Smal, C; Pellizza, L; Goldbaum, FA; Cicero, DO. Latin American Summit Meeting on Biological Crystallography and Complementary Methods. Campinas. Brasil. From 22nd to 24th of September 2014. Poster. - Crystal structures of BA41, BA42, and C24: three proteins of unknown function from the Antarctic bacterium Bizionia argentinensis. Otero, LH; Klinke, S; Cerutti, ML; Aran, M; Smal, C; Pellizza, L; Cicero, DO; Goldbaum, FA. 10th Argentinean Annual Meeting on Crystallography. Mar del Plata. Argentina. From 28th to 31st of October 2014. Poster. - Crystallization and preliminary X-ray characterization of the full-length bacteriophytochrome from the plant pathogen Xanthomonas campestris pv. campestris. Klinke, S; Otero, LH; Rinaldi, J; Sosa, Santiago; Goldbaum, FA; Bonomi HR. 10th Argentinean Annual Meeting on Crystallography. Mar del Plata. Argentina. From 28th to 31st of October 2014. Oral communication. - Structural characterization of BA41, a TPM superfamily member from the Antarctic bacterium Bizionia argentinensis. Cerutti, ML; Otero, LH; Klinke, S; Smal, C; Pellizza, L; Cicero, DO; Goldbaum, FA and Aran, M. 50th Annual Meeting Argentine Society for Biochemistry and Molecular Biology. Rosario. Argentina. From 11th to 14th of November 2014. Poster. - Antibiotic resistance in Staphylococcus aureus by allosteric control of Penicillin-Binding Protein 2a. Otero, LH; Fishovitz, J; Rojas-Altuve, A; Chang, M; Mobashery, S and Hermoso, JA. 50th Annual Meeting Argentine Society for Biochemistry and Molecular Biology. Rosario. Argentina. From 11th to 14th of November 2014. Oral communication. - Gaining insight into the activation mechanism of the response regulator NTRX from Brucella abortus. Fernández, J; Otero, LH; Klinke, S; Carrica, MC and Goldbaum, FA. 50th Annual Meeting Argentine Society for Biochemistry and Molecular Biology. Rosario. Argentina. From 11th to 14th of November 2014. Oral communication. Courses Post-grade: -13 courses (last years are shown) - Macromolecular Crystallography School. IQFR-Spain. (2012). - Computational structural biology - from data to structure to function. EMBO Practical Course. EMBL- Germany. (2013). - Macromolecular Crystallography School CCP4: from data processing to structure refinement and beyond. IFSC/USP-Brazil. (2014): - IUCr-UNESCO OpenLab: Resolution of crystal structures by X-ray diffraction. International Union of Crystallography and BRUKER. UDELAR-Uruguay. (2014). - Training about pre-clinical development of biological drugs. CABBIO. LNBio-Brazil. (2014). Grade: Over 20 (twenty). Most about Biochemistry, Microbiology, Biotechnology and Bioinformatics. Internships - Research Internship at “Laboratory of Expression and Folding of Proteins”. Ref.: PhD Mario Ermácora. Universidad Nacional de Quilmes. Buenos Aires. Argentina. 2 weeks. 2008. - Research Internship at “Group of Crystallography and Structural Biology”. Ref.: PhD Armando Albert. Institute of Physical Chemistry "ROCASOLANO" (IQFR), Madrid, Spain. 3 months. 2009-2010. - Research Internship at “Institute of Biotechnology”. Ref.: PhD Enrique Morett. Universidad Autónoma de México. Cuernavaca. Mexico. 2 weeks. 2010. - Research Internship at “Group of Crystallography and Structural Biology”. Ref.: PhD Armando Albert. Institute of Physical Chemistry "ROCASOLANO" (IQFR), Madrid, Spain. 1 month. 2010. PUBLICATIONS 16 full-articles: - Critical active-site residues identified by site-directed mutagenesis in Pseudomonas aeruginosa phosphorylcholine phosphatase, a new member of the haloacid dehalogenases hydrolase superfamily. Beassoni, PR; Otero, LH; Massimelli, MJ; Lisa, AT and Domenech, CE. Current Microbiology (2006) 53: 534-539. - Using a molecular model and kinetic experiments in the presence of divalent cations to study the active site and catalysis of Pseudomonas aeruginosa phosphorylcholine phosphatase. Beassoni, PR; Otero, LH; Lisa, AT; and Domenech, CE. Biochimica et Biophysica Acta (2008) 1784: 2038-2044. - Transition from octahedral to tetrahedral geometry causes the activation or inhibition by Zn2+ of Pseudomonas aeruginosa phosphorylcholine phosphatase. Otero, LH; Beassoni, PR; Lisa, AT and Domenech, CE. Biometals (2010) 23: 307-314. - Preparation and biophysical characterization of recombinant Pseudomonas aeruginosa phosphorylcholine phosphatase. Beassoni, PR; Pérez De Berti, F; Otero, LH; Risso, VA; Ferreyra RG; Lisa, AT; Domenech, CE and Ermácora, MR. Protein Expression and Purification (2010) 71: 153-159. - Crystallization and preliminary X-ray diffraction analysis of Pseudomonas aeruginosa phosphorylcholine phosphatase. Otero, LH; Beassoni, PR; Domenech, CE; Lisa, AT and Albert, A. Acta Crystallographica Section F (2010) F66: 957-960. - Site-directed mutations and kinetic studies show key residues involved in alkylammonium interactions and reveal two sites for phosphorylcholine in Pseudomonas aeruginosa phosphorylcholine phosphatase. Beassoni, PR; Otero, LH; Boetsch, C; Domenech, CE; Gonzales-Nilo, FD and Lisa, AT. Biochimica et Biophysica Acta (2011) 1814: 858-863. - Different effects of Mg2+ and Zn2+ on the two sites for alkylammonium compounds in Pseudomonas aeruginosa phosphorylcholine phosphatase. Otero, LH; Beassoni, PR; Boetsch,