9th ADVANCES AGAINST ASPERGILLOSIS AND MUCORMYCOSIS 27 - 29 February 2020 - Lugano, Switzerland POSTER LIST POSTER # ABSTRACT & AUTHORS 1 Surveillance of Mucorales resistance to azoles in the waste sorting industry: assessment of filtering respiratory protective devices and gloves LA Caetano*, M Dias, B Almeida, C Viegas 2 Aspergillus spp. and azole-resistance characterization on filtering respiratory protective devices from waste sorting industry C Viegas*, M Dias, B Almeida, P Gonçalves, C Verissimo, R Sabino, L Aranha Caetano 3 Antifungal susceptibility testing of clinical and hospital Aspergillus isolates at the nephrology ward of an Iranian training hospital K Diba*, H Fakhim, K Makhdoomi, N Javanmard, S Javanmard 4 Azole- and amphotericin B-resistant Aspergillus fumigatus strains isolated from clinical specimens and environment in Azerbaijan RM Huseynov*, SS Javadov, AA Kadyrova, B Bozdogan, E Oryashin, SM Askarova, BT Taqiyev, I Karalti, D Denning 5 Hmg1 gene mutation in triazole-resistant Aspergillus fumigatus clinical isolates without cyp51A gene mutations A Resendiz Sharpe*, R Merckx, P Verweij, J Maertens, K Lagrou 6 Emergence of azole resistant Aspergillus species: a consequence of environmental exposure to azole pesticides in agricultural fields of North India P Sen*, Mukund, M Vermani, J Shankar, P Vijayaraghavan 7 Isoeugenol as a potential antifungal molecule against azole resistant environmental isolates of Aspergillus fumigatus and their biofilm L Gupta*, P Sen, P Vijayaraghavan 8 Point mutations in the 14α-sterol demethylase Cyp51A or Cyp51C could contribute to azole resistance in Aspergillus flavus J Lucio*, I Gonzalez-Jimenez, O Rivero-Menendez, A Alastruey-Izquierdo, T Pelaez, L Alcazar-Fuoli, E Mellado 9 Antifungal activity of novel triazole, efinaconazole, and nine comparators against 354 molecularly identifiedAspergillus isolates, in vitro H Badali*, Z Taheri Rizi, M Abastabar, M Ilkit, JF Meis, MM Davoudi 10 Susceptibility profile of Mucoralean fungi isolated from the United States to current antifungal drugs H Badali*, C Gibas, D McCarthy, H Patterson, C Sanders, J Mele, H Fan, N.P Wiederhold 11 Clinical Aspergillus isolates causing aspergillosis in the last 20 years: an overview of aetiology and antifungal resistance to azoles and amphotericin B J Serrano*, E Reigadas, A Vena, M Machado, P Muñoz, P Escribano, J Guinea 12 Susceptibility patterns of contemporary Aspergillus fumigatus isolates from the United States to azole antifungals N Wiederhold*, H Badali, D McCarthy, H Patterson, C Sanders, J Mele, H Fan, C Gibas 13 Elucidating echinocandin resistance mechanisms in Mucor circinelloides A Garcia*, EY Huh, SC Lee 14 The role of pentraxin-3 in the immunometabolic regulation of antifungal immunity D Antunes*, V Aimanianda, C Duarte-Oliveira, SM Gonçalves, C Cunha, T Gonçalves, A Carvalho 9th ADVANCES AGAINST ASPERGILLOSIS AND MUCORMYCOSIS 27 - 29 February 2020 - Lugano, Switzerland POSTER # ABSTRACT & AUTHORS 15 Genome Wide Association Study (GWAS) for antifungal sensitivity in the opportunistic pathogen Aspergillus fumigatus S Zhao*, JR Fortwendel, A Watanabe, JG Gibbons 16 CRISPR / Cas9 genome editing technology to verify the contribution of known mutations in the cyp51A gene and its promoter to azole resistance in Aspergillus fumigatus T Umeyama*, T Inukai, M Tateno, S Yamagoe, S Takatsuka, Y Hoshino, K Ishino, Y Miyazaki 17 Hmg1 mutation conferring multi-azole resistance in Aspergillus fumigatus T Arai*, T Umeyama, T Inukai, A Watanabe, Y Miyazaki, K Kamei 18 CLSI versus EUCAST for azoles susceptibility - the necessity to unify D Pinheiro*, C Monteiro, M Maia, E Pinto 19 Antifungal susceptibility testing of clinical and community environment isolates of Aspergillus species J Chander*, N Singla, M Kaur, D Aggarwal2 20 In vitro evaluation of combination of Ibrexafungerp and azoles against Aspergillus spp. isolated from lung transplant recipients V Jagadeesan, E Driscoll, B Hao, S Barat, K Borroto-Esoda, D Angulo, C Clancy, M Nguyen* 21 D430G mutation of CYP51A in Aspergillus fumigatus causes azole-resistance H Majima, A Teppei, A Watanabe*, K Kamei 22 Antimicrobial susceptibility of Aspergillus fumigatus and Stenotrophomonas maltophilia biofilms: did they find strength in unity? L Roisin*, E Melloul, PL Woerther, J Guillot, E Dannaoui, F Botterel 23 Evaluation of drug susceptibility of Aspergillus species isolated from ICU of hospitals in vitro Ayat Nasrollahi Omran* 24 Mutations in A. fumigatus hmg1 which confer triazole resistance also alter sterol composition and increase multi-drug efflux pump gene expression JM Rybak*, W Ge, JE Parker, SL Kelly, NP Wiederhold, VM Bruno, PD Rogers, JR Fortwendel 25 Activity of diphenyl diselenide against Aspergillus isolates AM Melo*, VR Poester, L Munhoz, M Trápaga, B Roca, GB Klafke, RF Sabino, DA Stevens, MO Xavier 26 Molecular identification of Aspergillus isolates from Magellanic penguins AM Melo*, RP Silva-Filho, VR Poester, A von Grol, RF Sabino, DA Stevens, MO Xavier 27 In vitro and in vivo activity of manogepix/fosmanogepix, a novel antifungal with activity against Aspergillus and rare molds MD Huband, AS Ibrahim, T Gebremariam, DR Andes, PA Bien, KJ Shaw, MR Hodges* 28 Study and characterization of azole resistance in Aspergillus section Nigri A Pérez-Cantero*, A Martin-Vicente, L López-Fernández, J Guarro, JR Fortwendel, J Capilla 29 Does monitoring cyp51A-mediated triazole resistance in Aspergillus fumigatus by pyrosequencing lead to patient benefit? L Novak-Frazer*, D Hassan, S Hill, CB Moore, M Walczak, R Rautemaa-Richardson, MD Richardson 30 Azole resistant Aspergillus fumigatus from agricultural settings M Momany*, M Brewer, SE Kang, LG Sumabat, T Melie, B Mangum 9th ADVANCES AGAINST ASPERGILLOSIS AND MUCORMYCOSIS 27 - 29 February 2020 - Lugano, Switzerland POSTER # ABSTRACT & AUTHORS 31 The role of PTX3 in innate regulation of antifungal immunity in chronic pulmonary aspergillosis C Duarte-Oliveira*, S Ferreira, SM Gonçalves, D Antunes, A Mantovani, C Cunha, A Carvalho 32 Antifungal susceptibility profiles of olorofim (formerly F901318), and currently available systemic antifungals against mold and yeast phases of Talaromyces marneffei J Zhang*, HF Liu, LY Xi, YC Chang, KJ Kwon-Chung, S Seyedmousavi 33 Synergic anti-fungal activity of topical PC945, a novel inhaled azole, with systemic echinocandin on Aspergillus fumigatus in vitro human alveoli model L Daly*, KA Lucas, T Colley, P Strong, G Rapeport, K Ito 34 Altered A. fumigatus cell wall integrity by PC945, a novel inhaled azole D Armstrong-James*, T Colley, P Strong, G Rapeport, K Ito NOTE: This abstract has also been selected for Oral Presentation 35 Neutrophil ROS controls fungal growth and inflammation in phagocyte NADPH oxidase- deficient zebrafish TJ Schoen*, EE Rosowski, BP Knox, NP Keller, A Huttenlocher 36 In vivo and in vitro impairment of Th cell and neutrophil responses against Mucor irregularis in Card9 knockout mice LY Sun*, Z Wan, RY Li, J Yu 37 Modulation of TREM1 signaling in macrophages infected with Aspergillus fumigatus I González-Jiménez*, M Jerónimo-Albaladejo, L Bernal-Martínez, E Mellado, L Alcázar-Fuoli NOTE: This abstract has also been selected for Oral Presentation 38 Aspergillus fumigatus and Pseudomonas aeruginosa pulmonary co-exposure results in increased IL-17A, eosinophilia, and acute lung injury in allergic animals BN Steffan*, SA Hoselton, NP Keller, JM Schuh 39 Phagocytosis of Mucorales spores by lung epithelial cells and macrophages U Binder*, V Naschberger, C Lass-Flörl, D Wilflingseder 40 Evaluating the role of STAT3 in CD4+ T cells in susceptibility to invasive aspergillosis W Gohir*, L McTaggart, J Kus, T Mazzulli, D Kumar, A Humar, S Husain 41 Epithelial uptake of Aspergillus fumigatus spores drives efficient fungal clearancein vivo and is aberrant in Chronic Obstructive Pulmonary Disease (COPD) patients M Bertuzzi*, GJ Howell, R Fortune-Grant, X Du, J Smith, DD Thomson, L Gregson, BA Greser, NC Motsi, N Van Rhijn, M Demirbag, EM Bignell NOTE: This abstract has also been selected for Oral Presentation 42 Aspergillus fumigatus cell wall promotes apical airway epithelial recruitment of human neutrophils MB Feldman, RA Dutko, M Wood, RA Ward, HM Leung, RF Snow, DJ de la Flor, LM Yonkers, JL Reedy, GJ Tearney, H Mou, B Hurley, JM Vyas* 43 The antigens enolase, triosephosphate isomerase and heat shock protein HSS1 of Mucor circinelloides are recognized by sera from immunocompromised infected mice M Areitio, A Martin-Vicente, A Arbizu, A Antoran, L Aparicio-Fernandez, I Buldain, L Martin-Souto, X Guruceaga, A Rementeria, J Capilla, FL Hernando, A Ramirez-Garcia* 44 An immunoproteomics approach to identify Aspergillus fumigatus protein antigens in cystic fibrosis patients with allergic bronchopulmonary aspergillosis (ABPA) J Macheleidt, J Kruse, P Bacher, C Grehn, A Scheffold, C Schwarz, J Springer, J Löffler, H Einsele, O Kniemeyer*, A Brakhage 9th ADVANCES AGAINST ASPERGILLOSIS AND MUCORMYCOSIS 27 - 29 February 2020 - Lugano, Switzerland POSTER # ABSTRACT & AUTHORS 45 “Invasive aspergillosis on-a-chip” – a novel disease model to study Aspergillus fumigatus infection in the human lung TNM Hoang, Z Cseresnyés, S Hartung*, K Rennert, MT Figge, AS Mosig, M von Lilienfeld-Toal NOTE: This abstract has also been selected for Oral Presentation 46 Single cell approaches reveal the key dendritic cell subsets that coordinate allergic airway inflammation against the fungal allergen Aspergillus fumigatus P Cook*, S Brown, E Houlder, S Khan, G Tavernier, F Svedberg,