Sorbent Pens™ For Next Generation Headspace Analysis Featured Chromatograms Abundance 15 2e+08 1.8e+08 1.6e+08 1.4e+08 1.2e+08 1e+08 13 6 8e+07 2 6e+07 8 5 4e+07 4 11 14 3 2e+07 7 10 1 9 12 0 Time--> 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00 Brie Cheese Duplicate Analysis TIC: 16081501.D\data.ms SORBENT PEN™ #1 Abundance 1.1e+07 8 23 1e+07 9000000 8000000 2 7000000 6000000 5000000 4000000 3000000 19 4 2000000 7 15 *20 22 24 1000000 1 9 11 14 16 17 10 12 21 3 5 6 13 18 0 Time--> 10.00 15.00 20.00 25.00 30.00 35.00 SORBENT PEN™ #2 Abundance 8 23 1.1e+07 1e+07 9000000 8000000 2 7000000 6000000 5000000 4000000 3000000 19 4 7 15 2000000 22 14 17 24 1000000 1 9 10 11 *20 21 12 16 3 5 6 13 18 0 Time--> 10.00 15.00 20.00 25.00 30.00 35.00 *Difference in Dodecanoic acid, ethyl ester caused by difficulty in maintaining exact amount of cheese sample oxidation from run to run, and expected variations in sample homegeneity. Instrument: 5800-SPDU (Sorbent Pen Desorption Unit) 1. 4-Heptanone 14. 2-Undecanone Technique: VASE (Vacuum Assisted Sorbent Extraction) 2. 2-Heptanone 15. n-Decanoic acid Run date: August 15, 2016 3. Benzaldehyde 16. Decanoic acid, ethyl ester Sample description: Brie Cheese 4. 2-Octanone 17. 2H-Pyran-2-one, tetrahydro-6-pentyl- Weight of sample (g): 70g cheese in 140mL water, blended, 10.1025g taken 5. Hexanoic acid, ethyl ester 18. 2-Tridecanone Sample conditions: blended + vac(30sec) + 50°C + 3 hr equilibration 6. Benzeneacetaldehyde 19. Dodecanoic acid Desorb conditions: 260°C for 5 min. 7. 8-Nonen-2-one 20. Dodecanoic acid, ethyl ester Split Mode: Splitless 8. 2-Nonanone 21. 2H-Pyran-2-one, 6-heptyltetrahydro- Precolumn: DB1 5m length x 0.25mm ID, 0.25µm film 9. Nonanal 22. Tetradecanoic acid Column: DB1 30m length x 0.25mm ID, 0.5µm film 10. Octanoic acid 23. n-Hexadecanoic acid Carrier: He, 1.5cc/min. constant flow 11. Octanoic acid, ethyl ester 24. Hexadecanoic acid, ethyl ester Oven Temp: 40°C hold 5min., 6°C/min. to 95°C, 12. Decanal 10°C/min. to 140°C, 10°C/min. to 325 hold 5min. 13. n-Octanoic acid isopropyl ester GCMS: Agilent 7890B/5977A MS Operation: 34-450 amu, 1.8 scans/sec 1 Extremely Clean Blank After Strawberry Analysis TIC: 16080301.D\data.ms Abundance 9 3.5e+08 SORBENT PEN™ #1 3e+08 4 Abundance 2.5e+08 2 8 18 2e+08 1.1e+07 8 23 1 17 1e+07 1.5e+08 6 15 9000000 1e+08 10 14 19 8000000 5e+07 11 12 16 2 3 5 7 13 7000000 0 Time--> 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00 26.00 28.00 6000000 5000000 Abundance 4000000 3.5e+08 Blank run of the same Sorbent Pen directly after strawberry sample 3000000 19 4 3e+08 2000000 7 15 *20 2.5e+08 22 24 1000000 1 9 11 14 16 17 10 12 21 2e+08 3 5 6 13 18 0 1.5e+08 Time--> 10.00 15.00 20.00 25.00 30.00 35.00 1e+08 5e+07 0 Time--> 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00 26.00 28.00 (Instrument: 5800-SPDU (Sorbent Pen Desorption Unit Technique: VASE (Vacuum Assisted Sorbent Extraction) SORBENT PEN™ #2 Run date: August 3, 2016 1. Ethyl acetate 11. 3(2H)-Furanone, 4-methoxy-2,5-dimethyl- Sample description: Fresh Strawberries 2. Butanoic acid methyl ester 12. 1,6-Octadien-3-ol, 3,7-dimethyl- Abundance Weight of sample (g): 7.7425 3. Propanoic acid, 2-methyl-, ethyl ester 13. Octanoic acid, methyl ester 8 Sample conditions: 5 hours equilibration 23 4. Butanoic acid, ethyl ester 14. Methyl salicylate 1.1e+07 Desorb conditions: 260°C for 5 min. 5. Acetic acid, butyl ester 15. Octanoic acid, ethyl ester Split Mode: Splitless 1e+07 6. Butanoic acid, 2-methyl-, ethyl ester 16. Butanoic acid, octyl ester Precolumn: DB1 5m length x 0.25mm ID, 0.25µm film 7. 1-Butanol, 3-methyl-, acetate 17. Pentanoic acid, octyl ester Column: DB1 30m length x 0.25mm ID, 0.5µm film 9000000 8. Hexanoic acid, methyl ester 18. 1,6,10-Dodecatrien-3-ol, 3,7,11-trimethyl- Carrier: He, 1.5cc/min. constant flow 9. Hexanoic acid, ethyl ester 19. .gamma.-Dodecalactone 8000000 Oven Temp: 40°C hold 5min., 6°C/min. to 95°C, 10. 2-Hexen-1-ol, acetate, (Z)- 2 10°C/min. to 140°C, 15°C/min. to 325 hold 5 min. 7000000 GCMS: Agilent 7890B/5977A MS Operation: 34-450 amu, 1.8 scans/sec 6000000 5000000 4000000 Abundance 3000000 19 5000000 4 7 15 2000000 22 4000000 14 17 24 1000000 1 9 10 11 *20 21 5 1213 16 18 3 6 3000000 0 Time--> 10.00 15.00 20.00 25.00 30.00 35.00 2000000 *Difference in Dodecanoic acid, ethyl ester caused by difficulty in maintaining exact amount of cheese sample oxidation from run to run, and expected variations in sample homegeneity. 1000000 0 Time--> 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00 26.00 28.00 Instrument: 5800-SPDU (Sorbent Pen Desorption Unit) 1. 4-Heptanone 14. 2-Undecanone Technique: VASE (Vacuum Assisted Sorbent Extraction) 2. 2-Heptanone 15. n-Decanoic acid Run date: August 15, 2016 3. Benzaldehyde 16. Decanoic acid, ethyl ester Abundance Sample description: Brie Cheese 4. 2-Octanone 17. 2H-Pyran-2-one, tetrahydro-6-pentyl- Weight of sample (g): 70g cheese in 140mL water, blended, 10.1025g taken 5. Hexanoic acid, ethyl ester 18. 2-Tridecanone 5000000 Zoomed in baseline still reveals a completely clean chromatogram Sample conditions: blended + vac(30sec) + 50°C + 3 hr equilibration 6. Benzeneacetaldehyde 19. Dodecanoic acid Desorb conditions: 260°C for 5 min. 7. 8-Nonen-2-one 20. Dodecanoic acid, ethyl ester 4000000 Split Mode: Splitless 8. 2-Nonanone 21. 2H-Pyran-2-one, 6-heptyltetrahydro- Precolumn: DB1 5m length x 0.25mm ID, 0.25µm film 9. Nonanal 22. Tetradecanoic acid Column: DB1 30m length x 0.25mm ID, 0.5µm film 10. Octanoic acid 23. n-Hexadecanoic acid 3000000 Carrier: He, 1.5cc/min. constant flow 11. Octanoic acid, ethyl ester 24. Hexadecanoic acid, ethyl ester Oven Temp: 40°C hold 5min., 6°C/min. to 95°C, 12. Decanal 2000000 10°C/min. to 140°C, 10°C/min. to 325 hold 5min. 13. n-Octanoic acid isopropyl ester GCMS: Agilent 7890B/5977A 1000000 MS Operation: 34-450 amu, 1.8 scans/sec 0 Time--> 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00 26.00 28.00 2 Dark Chocolate Analysis TIC: 161117_04.D\data.ms Abundance 21 1.5e+08 1.4e+08 1.3e+08 24 1.2e+08 1.1e+08 1e+08 36 9e+07 15 8e+07 7e+07 6e+07 31 5e+07 22 26 4e+07 33 29 3e+07 20 28 30 2e+07 19 25 27 23 35 1e+07 32 39 34 37 38 40 Time--> 10.00 15.00 20.00 25.00 30.00 35.00 Abundance 18 15 17 8000000 (Instrument: 5800-SPDU (Sorbent Pen Desorption Unit 7000000 Technique: VASE (Vacuum Assisted Sorbent Extraction) 9 6000000 Run date: November 17, 2016 16 Sample description: Dark Chocolate Amount of sample (g): 1 5000000 Sample conditions: 40 mL vial with 15 hours equilibration at 25°C 13 Desorb conditions: 260°C for 5 min. 4000000 Split Mode: Splitless 6 12 14 2 8 Precolumn: DB1 5m length x 0.25mm ID, 0.25µm film Column: DB1 30m length x 0.25mm ID, 0.5µm film 3000000 Carrier: He, 1.5cc/min. constant flow 3 Oven Temp: 40°C hold 5 min., 10°C/min. to 325°C, 4 2000000 7 11 325 hold 5 min. (solvent delay at 6 min.) 10 GCMS: Agilent 7890B/5977A 1 MS Operation: 34-450 amu, 1.8 scans/sec 1000000 5 Time--> 7.50 8.00 8.50 9.00 9.50 10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 1. Propanoic acid, 2-methyl- 11. D-Limonene 21. Acetic acid, 2-phenylethyl ester 31. Benzophenone 2. Butanoic acid, 3-methyl- 12. Ethanone, 1-(1H-pyrrol-2-yl)- 22. Benzeneacetaldehyde, .alpha.-ethylidene- 32. Tetradecanoic acid, ethyl ester 3. Butanoic acid, 2-methyl- 13. Acetophenone 23. Vanillin 33 Caffeine 4. Heptanal 14. Pyrazine, 3-ethyl-2,5-dimethyl- 24. Benzoic acid, pent-2-yl ester 34. 2-Heptadecanone 5. Pyrazine, 2,5-dimethyl- 15. Pyrazine, tetramethyl- 25. Tetradecane 35. Hexadecanoic acid, methyl ester 6. Benzaldehyde 16. 2-Nonanone 26. 5-Methyl-2-phenyl-2-hexenal 36. Di-sec-butyl phthalate 7. Pentanoic acid, 4-methyl- 17. Nonanal 27. Pentadecane 37. 9-Octadecenoic acid, methyl ester 8. Hexanoic acid 18. 1,6-Octadien-3-ol, 3,7-dimethyl- 28. Butylated Hydroxytoluene 38. Linoleic acid ethyl ester 9. Pyrazine, trimethyl- 19. Octanoic acid, ethyl ester 29.