Programme & Abstract Book CHROMATIN STRUCTURE & FUNCTION Nassau, Bahamas 15th - 18th November 2005 Organised By: Tony Kouzarides and Abcam Programme & Abstract Book The second CHROMATIN STRUCTURE & FUNCTION Nassau, Bahamas 15th - 18th November 2005 Organizers: Tony Kouzarides (University of Cambridge) and Abcam Table of contents Timetable . .Page 2 Conference Programme . .Page 3 Poster Index . .Page 8 Abstracts - Oral . .Page 18 Abstracts - Poster . .Page 57 Resort Information . .Page 173 Disclaimer: Material contained within this booklet should be citied only with permission from the author(s). No live recording or photography is permitted during the oral or poster sessions. Copyright © 2005 Abcam, All Rights Reserved. The Abcam logo is a registered trademark. All information / detail is correct at time of going to print. 1 Chromatin Structure & Function Nassau, Bahamas, 15th - 18th November 2005 Timetable Tuesday 15th November 18:00 Keynote Speaker 19:00 Poolside dinner reception Wednesday 16th November 09:00 - 10:30 Oral presentations Drinks break 11:00 - 12:30 Oral presentations Lunch and free time 16:00 - 17:30 Oral presentations Drinks break 18:00 - 21:00 Poster session and evening buffet Thursday 17th November 09:00 - 10:30 Oral presentations Drinks break 11:00 - 12:30 Oral presentations Lunch and free time 16:00 - 17:30 Oral presentations Drinks break 18:00 - 19:30 Oral presentations 20:00 Beach Barbeque Friday 18th November 09:00 - 10:30 Oral presentations Break 11:00 - 12:15 Oral presentations Lunch and departures Keynote Speaker Sponsors: 2 Conference Programme Conference Programme Tuesday 15th November Chair: Tony Kouzarides Keynote Speaker 18:00 - 19:00 Danny Reinberg . .Page 18 Histone marks and chromatin states Wednesday 16th November Chair: Ali Shilatifard 09:00 - 09:30 Shelley Berger . .Page 19 Factor and histone covalent modifications in genome regulation 09:30 - 09:45 Brad Bernstein . .Page 20 Genomic studies of chromatin modifications in normal and malignant cells 09:45 - 10:00 Hendrik Stunnenberg . .Page 21 Histone modification patterns associated with X-inactivation and escape 10:00 - 10:30 Sandra Hake . .Page 22 Beyond the double helix: Understanding the functional importance of histone H3 and its variants Break 11:00 - 11:30 Yang Shi . .Page 23 A new kid on the chromatin block: identification of the first histone demethylase LSD1 and its regulation 11:30 - 11:45 Roland Schuele . .Page 24 LSD1 demethylates repressive histone marks to promote androgen receptor dependent transcription 11:45 - 12:15 Tony Kouzarides . .Page 25 Proline isomerisation of histone H3 regulates gene expression 12:15 - 12:30 Victoria Lunyak . .Page 26 ESET dependent H3-K9 methylation upon restructuring of chromosomal domains during development. Lunch and free time 3 Chromatin Structure & Function Nassau, Bahamas, 15th - 18th November 2005 Wednesday 16th November (continued) Chair: Craig Peterson 16:00 - 16:30 Yi Zhang . .Page 27 Histone modifications in polycomb silencing and cancer 16:30 - 16:45 Patrick Grant . .Page 28 Generation and recognition of histone modifications by the SAGA and SLIK HAT complexes in health and disease 16:45 - 17:00 Vincent Geli . .Page 29 Structure and function of the Set1 RNA recognition motif 17:00 - 17:30 Ali Shilatifard . .Page 30 Yeast COMPASS points the way to human MLL and its role in pathogenesis of leukemia Break 18:00 - 21:00 Posters Thursday 17th November Chair: Shelley Berger 09:00 - 09:30 Kevin Strule . .Page 31 Abstract title to be confirmed 09:30 - 09:45 Oliver Rando . .Page 32 Genome-scale characterization of chromatin structure 09:45 - 10:00 Rolf Sternglanz . .Page 33 Histone mutations that suppress the silencing defects of a yeast sir3 Ala2Gly mutation 10:00 - 10:15 Antonin Morillon . .Page 34 Histone variant HT2A-Z promotes promoter-proximal nucleosome dissociation upon transcription activation in S. cerevisiae. 10:15 - 10:30 Sharon Dent . .Page 35 New functions for histone modifying enzymes Break 4 Conference Programme Thursday 17th November (continued) Chair: Shelley Berger 11:00 - 11:30 Jerry Workman . .Page 36 Set2 methylates histone H3 K36 providing transcriptional memory that signals Rpd3S to deacetylate histones in transcribed regions to suppress spurious intragenic transcription 11:30 - 11:45 Nevan Krogan . .Page 37 Protein complexes and pathways involved in chromatin function 11:45 - 12:00 Bruno Amati . .Page 38 Epigenetic determinants of Myc binding to the human genome 12:00 - 12:15 Francois Fuks . .Page 39 A direct mechanistic link between the Polycomb Group protein EZH2 and DNA methyltransferases 12:15 - 12:30 Julie Ahringer . .Page 40 Chromatin regulation and sumoylation in the inhibition of Ras induced vulval development in C. elegans Lunch and free time Chair: Sharon Dent 16:00 - 16:30 Peter Becker . .Page 41 Site-specific acetylation defines an embryonic form of ISWI that associates with mitotic chromatin 16:30 - 16:45 Stephen Rea . .Page 42 hMOF complex is required for histone H4 lysine 16 acetylation in mammalian cells 16:45 - 17:00 Susana Gonzalo . .Page 43 DNA methyltransferases control telomere length in mammalian cells 17:00 - 17:30 Craig Peterson . .Page 44 Chromatin remodeling: Regulation of chromatin higher-order folding and DNA repair Break 5 Chromatin Structure & Function Nassau, Bahamas, 15th - 18th November 2005 Thursday 17th November (continued) Chair: Sharon Dent 18:00 - 18:30 Bob Kingston . .Page 45 Remodeling chromatin without covalent modification 18:30 - 18:45 Lianna Johnson . .Page 46 DNA and histone methylation in Arabidopsis 18:45 - 19:00 Eric Miska . .Page 47 Short RNAs in C. elegans development and human cancer 19:00 - 19:30 Ramin Shiekhattar . .Page 48 Integrator, a multiprotein mediator of small nuclear RNA processing associates with the C-terminal repeat of RNA polymerase II Friday 18th November Chair: Kevin Struhl 09:00 - 09:30 Kami Ahmad . .Page 49 Abstract title to be confirmed 09:30 - 09:45 Jacques Cote . .Page 50 Dissection of the NuA4 HAT complex and crosstalk with other chromatin modifications and remodelers 09:45 - 10:00 Benoit Guillemette . .Page 51 H2A.Z is globally localized to the promoters of inactive genes and regulates nucleosome positioning 10:00 - 10:30 Karolin Luger . .Page 52 Structure and function of yeast nucleosome assembly protein 1 Break 11:00 - 11:30 Wolf Reik . .Page 53 Regulation of imprinting and epigenetic reprogramming in mammals 11:30 - 11:45 Aidan Doherty . .Page 54 Structure and function of the Jumonji C Domain 6 Conference Programme Friday 18th November (continued) Chair: Kevin Struhl 11:45 - 12:00 Andreas Ladurner . .Page 55 Human chromatin as a molecular target of cellular NAD metabolites 12:00 - 12:15 Jef Boeke . .Page 56 Comprehensive mutagenesis of sites of histone modification 7 Chromatin Structure & Function Nassau, Bahamas, 15th - 18th November 2005 Poster index Abstract 1 Ted Abel . .Page 57 The role of CBP and histone acetylation in memory storage and synaptic plasticity Abstract 2 Melissa Adkins . .Page 58 Spt6-mediated nucleosome reassembly is required for transcriptional repression of the PHO5 gene Abstract 3 Adriana Alejandro-Osorio . .Page 59 The role of the histone deacetylase Rpd3p in coordinating the environmental stress response in yeast Abstract 4 Erik Andersen . .Page 60 MET-1 and MET-2, two putative histone methyltransferases, are redundantly required for proper vulval cell fate specification Abstract 5 Anthony Argentaro . .Page 61 Structure/function studies of the PHD-like domain of the chromatin-remodelling protein, ATRX Abstract 6 Koh Meng Aw Yong . .Page 62 The presence of EBV oriP within a transcription unit inhibits replication as well as transcription Abstract 7 Slobodan Barbaric . .Page 63 Two nucleosomes at the yeast PHO84 promoter demonstrates differential requirement for chromatin remodelling activities Abstract 8 Vivian Bardwell . .Page 64 Polycomb group and SCF ubiquitin ligases are found in a BCL6 corepressor complex Abstract 9 Ralph Bash . .Page 65 AFM recognition imaging: using antibodies to track proteins during in vitro remodeling Abstract 10 Juraj Bies . .Page 66 Pc2, the polycomb group protein recruits the c-Myb into PcG bodies and inhibits its activity Abstract 11 Caroline Bouchard . .Page 67 c-Myc induces localized H2A.Z exchange in the promoters of target genes 8 Poster Index Abstract 12 Ray Camahort . .Page 68 Genome-wide localization of the budding yeast histone variant Cse4 Abstract 13 Kendra Cann . .Page 69 Multiple regions of the DNA-damage response protein TLS/FUS can regulate its relocalization to the nucleolei following transcriptional inhibition Abstract 14 Rafael Casellas . .Page 70 Effect of DNA double-stranded break repair on gene expression Abstract 15 Beverley Chilton . .Page 71 It Takes Two to Tango: autorepression by RUSH-1α/β Abstract 16 Sung Hee Baek . .Page 72 A chromatin remodeling complex in regulation of a metastasis suppressor gene Abstract 17 Pierre Close . .Page 73 Elongator depletion causes defective transcript elongation of genes that regulate cell motility Abstract 18 Megan Cole . .Page 74 Genome-wide map of nucleosome acetylation and methylation in yeast Abstract 19 Jean-Francois Couture . .Page 75 Structural insights into lysine multiple methylation by SET domain methyltransferases Abstract 20 Qi Dai . .Page 76 Snail-mediated repression in Drosophila requires Ebi and histone deacetylation Abstract 21 Ana D’Alessio . .Page 77 The kinetics of chromatin-driven active DNA demethylation in living cells Abstract 22 Jan-Hermen Dannenberg