T611135 Anti-EIF2S2/EIF2B Rabbit pAb Order 021-34695924 [email protected] Support 400-6123-828 50ul [email protected] 100 uL √ √ Web www.ab-mart.com.cn Product Information Description EIF2S2 rabbit polyclonal Protein full name Eukaryotic translation initiation factor 2 subunit 2 Synonyms eIF-2-beta, Eif2s2, Eukaryotic translation initiation factor 2 subunit beta Immunogen Recombinant protein corresponding to Mouse EIF2S2 Isotype IgG Purity Affinity purification Subcellular location Cytoplasm Predicted MW. 38 kDa Observed MW. 50 kDa Uniprot ID P20042, Q99L45 Applications Applications WB IHC IF Species Human, Mouse, Rat Human, Rat Human, Rat Dilution 1: 500-1: 1000 1: 1000-1: 2000 1: 500-1: 1000 Positive tissue lung, colon, small colon, lung, placenta colon, brain intestine Background Eukaryotic translation initiation factor 2 (eIF2) is composed of three subunits, eIF2 alpha, eIF2 beta (EIF2S2), and eIF2 gamma, which are present in equal molar amounts. eIF2 beta plays a central role in the maintenance of what is generally considered a rate-limiting step in mRNA translation. In the early steps of protein synthesis, eIF2 beta binds GTP and Met-tRNA and transfers Met-tRNA to the 40S ribosomal subunit. At the end of the initiation process, GTP bound to eIF2 beta is hydrolyzed to GDP and the eIF2/GDP complex is released from the ribosome. The exchange of GDP bound to eIF2 beta for GTP is a prerequisite to binding Met-tRNA and is mediated by eIF2 beta, which recycles the eIF2 complex for another round of initiation. Images Western blot analysis of EIF2S2 (T611135M) at dilution of 1: 1000 Lane 1: HT29 cell lysate Lane 2: L1299 cell lysate Lane 3: CaCO2 cell lysate Lane 4: Mouse lung tissue lysate Lane 5: Mouse colon tissue lysate Lane 6: Mouse small intestine tissue lysate Lane 7: Rat lung tissue lysate Immunohistochemistry analysis of paraffin- embedded human colon using EIF2S2 (T611135M) at dilution of 1: 1000 Immunohistochemistry analysis of paraffin- embedded rat lung using EIF2S2 (T611135M) at dilution of 1: 2000 ? Immunohistochemistry analysis of paraffin- embedded rat placenta using EIF2S2 (T611135M) at dilution of 1: 1000 Immunofluorescent analysis of paraffin- embedded human colon using EIF2S2 (T611135M) at dilution of 1: 1000 Immunofluorescent analysis of paraffin- embedded rat brain using EIF2S2 (T611135M) at dilution of 1: 1000 Storage Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. NOTE: This product is intended for research only. .