1/11/2017 AUTHENTICATION OF INTRODUCTION FICUS DELTOIDEA VARIETY KUNSTLERI FOR DELTOZIDE METHOD PHYTOPHARMACEUTICAL PRODUCT RESULT Anee Suryani Sued, Zainah Adam, Fazliana Mohd Saaya, Bohari Yaacob, Hazlina Ahmad Hassali, Daryl Jesus Arapoc, Mohamed Zaffar Ali Mohamed Amiroudine, Rosniza Razali CONCLUSION Phytopharmaceutical Technology Group, Medical Technology Division INTRODUCTION Plant morphology Microscopic examination METHOD Authenticity Ash value Foreign organic matter Purity Moisture content RESULT Extractive values Crude fibre CONCLUSION Assay Chemical profiling Biological profiling unaided sense of sight, smell or taste with the aid of hand-lense (4-20X magnification) for visual identification use plant in whole / uncut form Macroscopy DNA observation fingerprinting Analysis based on attributes such as: Defined morphological and/or anatomical characteristics of the whole plant or individual plant parts (leaf, flower, fruit, seed, root & rhizome, bark) Characteristics colour, fracture, smell or taste Identification is achieved by: Positive comparison of morphological characteristics with authenticated Microscopy Chemical plant reference material examination analysis Authoritative technical reference description 1 1/11/2017 Use of higher magnification than provided by a hand lens Based on Literature Review Use of special light and staining techniques Identification based on microscopic observation of cell & tissue structure: Defined histological characteristics of plant parts (leaf, flower, fruit, seed, root & rhizome, bark) Defined staining or microscopic chemical reaction Identification is achieved by: kunstleri trengganuensis bilobata angustifolia deltoidea intermedia motleyana Use of a validated method Authoritative technical description of established microscopic characteristics Figure 1: The matured leaf shapes of Ficus deltoidea. Pictures showing the adaxial (upper) followed by abaxial (lower) surfaces. A - var. kunstleri, B- var. trengganuensis, C- var. bilobata, D- var. angustifolia, E- var. deltoidea, F- var. intermedia, G- var. motleyana. Reference: Nur Fatihah HN et al. (2014). Leaf morphology & anatomy of 7 varieties of Ficus deltoidea (Moraceae). Turk. J. Bot. 38:677-685. INTRODUCTION At Sri Moro Mas cotek Plantation, Batu Pahat, Johor METHOD RESULT CONCLUSION Kedah - Telinga kera Tapak itik Delima sudip Syiling Telinga gajah Kelantan Inflorescence & leaf tree root floral morphology shape woody type type size suffruticose morphology characteristics morphology herbaceous Detailed illustration Visual documentation Photographic image 2 1/11/2017 INTRODUCTION METHOD RESULT CONCLUSION Natural habit – epiphytic shrub up to 2 meter tall Domesticated on ground Barks are gray colour Site Visit Plant Identification Sample Collection Produce latex-like material within their vasculators Leaf shape – obovate Abaxial surface – shiny green with golden specks Adaxial surface – olive brown with dark spots in between main veins branching Fruit is called fig Small in size – 1 cm diameter Change colour from green to yellow to pinky red when ripening Herbarium Sample & Botanical drawing of Ficus deltoidea Jack variety kunstleri King (Corner) To capture the macroscopic morphology of the plant parts in detail Herbarium Sample Botanical Drawing Scientific identification Ficus deltoidea Jack var kunstleri King (Corner): Botanical Name Shrub; Leaves dark green, light green below with black Ficus deltoidea Jack variety kunstleri (King) Corner glands conspicuous in vein axil; Fruit green, yellowish apically Taxanomic Classification Kingdom : PLANTAE Phylum : TRACHEOPHYTA Class : MAGNOLIOPSIDA Order : ROSALES Family : MORACEAE Genus : FICUS Ficus deltoidea var kunstleri: A – B, whole plant; Spesies : Ficus deltoidea Ficus deltoidea var kunstleri C, detail lower leaf surface with black spot in taken at Mas cotek Plantation, Variety : Ficus deltoidea Jack var. kunstleri (King) Corner between the leaf stem; D, fruit; E, longitudinal Masmoro Enterprise, Batu Pahat, Johor cross-section; F, male; and G, female flowers. 3 1/11/2017 Transverse section of Ficus deltoidea Jack variety kunstleri King (Corner) leaf - LAMINA Transverse section of Ficus deltoidea Jack variety kunstleri King (Corner) leaf - MIDRIB Figure 4.3: Transverse section (TS) of Ficus deltoidea var kunstleri leaf. A, TS midrib, the presence of Figure 3: A, TS lamina; B, TS margin (the presence of sclerenchyma cell on the tip of the margin); phloem medullary and sclerenchyma cell with completely ensheathing vascular bundle C, calcium oxalate crystal (present in the palisade mesophyll); D, cystolith cell on abaxial of lamina. Scale: A & D = 100 µm; B & C = 10 µm Transverse section of Ficus deltoidea Jack variety kunstleri King (Corner) leaf - PETIOL INTRODUCTION METHOD RESULT Figure 4.4: Transverse section (TS) of Ficus deltoidea var kunstleri leaf. A, TS petiole; B, Epicuticular wax; CONCLUSION C, Collenchyma cell and mucilage cell present in the parenchyma cortex and emphulur. Scale: B= 10 µm & C = 20 µm Monograph of Ficus deltoidea Jack variety kunstleri King (Corner) Botanical and macroscopic morphology of Ficus deltoidea Microscopic description of Ficus deltoidea plant parts Chemical Analysis Reports on Medicinal Use Toxicology Information 4 .