HS805-AMB Cold ZN – Kinyoun Stain Pack counterstain: Alcoholic Methylene Blue Stain pack consists of: HS180 – Carbol Fuchsin (Kinyoun) HS787 – ZN Decolouriser HS500 – Methylene Blue (1% w/v alcoholic) Issue 3/08.2014 SDS Ref: HS180 Carbol Fuchsin ( Kinyoun) v3 Review Date: 20.08.2014 Print Date: 20.08.2014 SAFETY DATA SHEET This Safety Data Sheet contains information concerning the potential risks to those involved in handling, transporting and working with this material, as well as describing potential risks to the environment. This information must be made available to those who may come into contact with the material or are responsible for the use of the material. This Safety Data Sheet is prepared in accordance with formatting described in the REACH Regulation (EC) No 1907/2006 and (EU) No 453/2010 SECTION 1. Identification of the substances/mixture and of the company/undertaking . 1.1 Product Identifier Product Name: Carbol Fuchsin ( Kinyoun ) Product Number: HS180 Brand: TCS Biosciences IUPAC name not determined 1.2 Relevant identified uses of the substance or mixture and uses advised against Identified uses: Laboratory Reagent 1.3 Details of the supplier of the safety data sheet Company: TCS Biosciences Ltd Botolph Claydon, Buckingham, MK18 2LR, UK Telephone: +44 (0) 1296 714222 Email address: [email protected] Web address: www.tcsbiosciences.co.uk 1.4 Emergency telephone number In case of emergency: 24hr contact number: +44 (0)1296 711205 SECTION 2. Hazards Identification . 2.1 Classification of the substance or mixture Classification in accordance with the Classification Labelling and Packaging Regulation EC (no) 1272/2008: Flammable Liquid ( Category 3) Acute inhalation toxicity, (Category 3) Carcinogenic (Category 2) Mutagenic ( Category 2) Skin corrosion (Category 1B) Specific target organ toxicity — repeated exposure- Category 2 Classification according to Directive 1999/45/EEC & 67/548/EEC and its amendments Flammable Harmful by inhalation, in contact with the skin and if swallowed.. Limited evidence of carcinogenic effect Possible risk of irreversible effects. Causes Burns 24hr contact number: +44 (0)1296 711205 www.tcsbiosciences.co.uk Page 1 of 9 SDS Ref: HS180 Carbol Fuchsin ( Kinyoun) v3 Review Date: 20.08.2014 Print Date: 20.08.2014 2.2 Label Elements Labelling in accordance with the Classification Labelling and Packaging Regulation EC (no) 1272/2008 Pictogram: Signal Word: Danger Hazard Statement(s): H226 Flammable liquid and vapour H331 Toxic if inhaled. H314 Causes severe skin burns and eye damage H341 Suspected of causing genetic defects H351 Suspected of causing cancer Precautionary Statement(s) P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking P260 Do not breathe dust/fume/gas/mist/vapours/spray. P280 Wear protective gloves/protective clothing/eye protection/face protection. P303+P361+P353 IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P308+P313 IF exposed or concerned: Get medical advice/attention. Labelling in accordance with Dangerous Preparations Directive 1999/45/EEC & 67/548/EEC and its amendments: Hazard symbol(s): . Flammable Corrosive Harmful Other hazards: Dyes and Stains by their physical nature may result in permanent staining if in contact with skin and clothing. R-phrase(s) R 10 Flammable R 20/21/22 Harmful by inhalation, in contact with skin and if swallowed. R 34 Causes burns. R 40 Limited evidence of a carcinogenic effect. R 68 Possible risk of irreversible effects. S-phrase(s): S16 Keep away from sources of ignition – No smoking S23 Do not breathe vapour. S24/25 Avoid contact with skin and eyes S36/37/39 Wear suitable protective clothing, gloves and eye/face protection 24hr contact number: +44 (0)1296 711205 www.tcsbiosciences.co.uk Page 2 of 9 SDS Ref: HS180 Carbol Fuchsin ( Kinyoun) v3 Review Date: 20.08.2014 Print Date: 20.08.2014 S45 In case of accident or if you feel unwell, seek medical advice immediately (show label where possible) SECTION 3. Composition/Information on Ingredients . 3.2 Mixtures Synonyms: None Carbol Fuchsin (Kinyoun) Component CAS-No. EC No Conc Classification in accordance with CLP 1272/2008 Ethanol 64-17-5 200-578-6 <20% Flam. Liq 2 H225; Methanol 67-56-1 200-659-6 <1% Flam. Liq 2; H225 Acute tox 3;H301,H311,H331 STOT SE1 :H370 Phenol 108-95-2 203-632-7 ~6% Muta 2; H341; Acute tox 3 H331,H311,H301 STOT.RE2; H373 Skin corr 1B;H314 Basic Fuchsin 632-99-5 211-189-6 ~3% Acute tox 4 ; H302 Carc 2; H351 Component EC No CAS-No Content Classification in accordance with DSD 67/548/EEC Ethanol 200-578-6 64-17-5 <20 F; R11 Methanol 200-659-6 67-56-1 <1% F; T; R11; R23/24/25 R39/23/24/25 Phenol 203-632-7 108-95-2 ~6% Muta Cat 3 : R68 T: R23/24/25, Xn:R48/20/21/22 C:R34 Basic Fuchsin 211-189-6 632-99-5 ~3% Xn; R22, Carc. Cat 3; R40 Refer to section 16 for additional classification information. SECTION 4. First Aid Measures . 4.1 Description of first aid measures If exposed keep patient calm and seek immediate medical advice where irritation persists. Have product container or label at hand. Show this safety data sheet to doctor/physician in attendance. If inhaled IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. If not breathing, give artificial respiration. Get medical advice/attention immediately. In case of skin contact IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Wash with plenty of soap and water. Wash contaminated clothing before reuse. If skin irritation occurs, seek medical advice/attention. In case of eye contact IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue Rinsing. If eye irritation persists get medical advice/attention. If swallowed Do NOT induce vomiting. Rinse out mouth with water provided person is conscious. Never give anything by mouth to an unconscious person. Get medical advice/attention if irritation persists. 24hr contact number: +44 (0)1296 711205 www.tcsbiosciences.co.uk Page 3 of 9 SDS Ref: HS180 Carbol Fuchsin ( Kinyoun) v3 Review Date: 20.08.2014 Print Date: 20.08.2014 4.2 Most important symptoms and effects, both acute and delayed No data available. 4.3 Indication of immediate medical attention and special treatment needed No data available. SECTION 5. Fire-Fighting Measures . 5.1 Suitable extinguishing media Use alcohol-resistant foam or fine water spray, dry chemical powder or carbon dioxide For large fires immediately alert fire emergency services. Evacuate personnel to safe area. 5.2 Special hazards arising from the substance or mixture May emit toxic fumes under fire. 5.3 Precautions for fire-fighters Wear self-contained breathing apparatus /protective clothing. Avoid contact with skin and eyes. 5.4 Further information No data available. SECTION 6. Accidental Release Measures . 6.1 Personal precautions, protective equipment and emergency procedures Observe all warning labels on container. Avoid contact with skin and eyes. Avoid breathing dust/fumes/gas/mist/vapours/spray; ensure adequate ventilation. Wear suitable protective clothing, gloves and eye/face protection. Wash hands thoroughly after handling. Evacuate personnel to safe area. 6.2 Environmental precautions Do not let undiluted product or large quantities enter drains or water course. Inform responsible authorities as appropriate. 6.3 Methods and materials for containment and cleaning up Absorb spillage with appropriate absorbent material e.g. vermiculite or sand; and dispose into suitably labelled closed containers for disposal according to local regulations. Wash spillage site with water and appropriate detergent. 6.4 Reference to other sections For disposal refer to section 13. SECTION 7. Handling and Storage . 7.1 Precautions for safe handling Observe all warning labels on container. Ensure adequate ventilation. Wear appropriate personal protective equipment provided. Avoid formation of dust and aerosols. Avoid prolonged or repeated exposure. 7.2 Conditions for safe storage, including any incompatibilities Store in a cool, dry, well-ventilated place. Replace container lid after use and keep container tightly closed to prevent leakage. 7.3 Specific end uses No data available SECTION 8. Exposure Controls//Personal Protection . 8.1 Control parameters 24hr contact number: +44 (0)1296 711205 www.tcsbiosciences.co.uk Page 4 of 9 SDS Ref: HS180 Carbol Fuchsin ( Kinyoun) v3 Review Date: 20.08.2014 Print Date: 20.08.2014 Components with workplace control parameters TWA Time Weighted Average Concentration (Long Term Exposure Limit) STEL Short Term Exposure Limit LTEL Long Term Exposure Limit Methanol can be absorbed through the skin. The assigned substances are those for which there are concerns that dermal absorption will lead to systemic toxicity. Workplace Exposure Levels: WEL EH40/2005 Methanol CAS No. 67-56-1 . 3 TWA STEL UK 15-min: 250ppm 333 mg/m 3 TWA LTEL Europe 8-h: 200ppm 266 mg/m Workplace Exposure Levels: WEL EH40/2005 Ethanol CAS No. 64-17-5 . TWA LTEL UK 8-hour: 1000ppm 1920 mg/m3 Workplace Exposure Levels: WEL EH40/2005 Phenol CAS-No: 108-95-2 TWA LTEL UK 8-hour: 2ppm 4 mg/m3 Can be absorbed through skin. The assigned substances are those for which there are concerns that dermal absorption will lead to systemic toxicity. Eu Commission Directive 2009/161/EU Phenol CAS-No: 108-95-2 TWA STEL UK 15-min: 4ppm 16 mg/m3 Identifies the possibility of significant uptake through the skin. 8.1.4 DNEL values (worker) Phenol EC No: 203-632-7 CAS-No: 108-95-2 Route of Exposure Exposure Time Effect Value Dermal Long term (Chronic) Systemic 1.23 mg/kg bw/d 8.2 Exposure controls Appropriate engineering controls Use process enclosures, local exhaust ventilation, or other engineering controls to keep airborne levels below recommended exposure limits.