MOTILE CELL Characters and Character States Location code / cell type ZO, zoospore Flagella apparatus Kinetosome Electron-opaque material in core in kinetosome 0, absent (everything else); 1, present (Kappamyces). Electron-opaque material in axoneme core and between axoneme and flagellar membrane 0, absent; 1, present (many Chytridiales). Kinetosome characters Scalloped ring within kinetosome, extensions of the A, B, or C microtubule Flagellum coating 0, absent; 1, present (Polyphagus euglenae). Number of flagella 0, one 1, multiple Kinetosome-associated structures (KAS) Kinetosome support 0, absent 1, kinetosome props; 2, broken kinetosome props (Olpidium radicale); 3, saddle-like structure surrounding kinetosome (Neocallimastigales) Kinetosome-associated plates 0, absent; 1, present. Kinetosome-associated spur 0, absent; 1, present. Kinetosome-associated shield 0, absent; 1, present. Kinetosome-associated veil 0, absent; 1, present. Anteriorly oriented kinetosome microtubule organizing center (MTOC) (Spizellomycetales) 0, absent; 1, simple solid, not laminate, only one part (Spizellomyces); 2, stacked plates with radiating anteriorly oriented microtubules (Powellomyces) 3, stacked plates with laterally anteriorly oriented microtubules (Gaerteromyces) 4, compound, two in stacked arrangement (Kochiomyces) 5, tripartitalcar rod with 3 lobes in cross-section Primary microtubule roots 0, absent; 1, 2-5 parallel microtubules are stacked with space between them, no connectors (Rhyzophydium); 2, approx. 6-7 parallel microtubules chord-like; 3, more than 20 parallel microtubules, bundled and have connectors/linkers between them (Nowakowskiella); 4, microtubules posterior to anterior, parallel to kinetosome triplets (Batracomyces – not separated like Rhysophydium). Striated rhizoplast 0, absent; 1, present. Flagellar rootlet absolute configuration 0, absent; 1, 20 degrees left; 2, 10 degrees right; 3, 50 degrees left. Flagella rootlets 0, absent; 1, of 2-16 microtubules connecting kinetosome to rumposome (Chytridiales); 2, of 27 microtubules in groups of 3 extending from anterior of kinetosome (Blastocladiales); 3, root of two parts: a striated disk extending part way around kinetosome, and microtubules extending anteriorly from kinetosome; (Monoblepharidales); 4, extending anteriorly without regular arrangement from spur on kinetosome; 5, microtubules in cone arising from MTOC anterior end of kinetosome. Spatial dimension of flagellar rootlet microtubules 0, absent; 1, diverging; 2, longitudinally stacked in single file; 3, layered and cross-linked; 4, layered and not cross-linked; 5, layered and embedded in electron-opaque material. Non-flagellated centriole Non-flagellated centriole (NFC) 0, absent; 1, parallel to kinetosome; 2, oblique to kinetosome; 3, at right angle to kinetosome. Length of non-flagellated centriole (NFC) compared to centriole (* caveat) 0, short (Monoblepharidales, Kappamyces); 1, equal; 2, long (Lacustromyces). Kinetosome-NFC bridge 0, absent; 1, fibers transverse; 2, fibers parallel; 3, homogeneous anterior. NFC-associated veil 0, absent; 1, present. Other cell features Paracrystalline body 0, absent; 1, present (Blastocladiales, Chytridiomyces clade and Phlyctochytrium). Ribosome organization 0, scattered ribosomes; 1, core area of compacted ribosomes partially or completely enclosed by endoplasmic reticulum; 2, in nuclear cap; 3, loosely enclosed by endoplasmic reticulum and surrounding nucleus; 4, in aggregates at anterior of cell. Mitochondria 0, absent (hydrogenosomes); 1, single; 2, multiple. Cisterna 0, absent; 1, simple; 2, fenestrated (rumposome). Annular cisterna 0, absent; 1, present (Monoblepharidales). Paraxonemal rod 0, absent; 1, present. Water expulsion vacuole 0, absent; (Chytridiales); 1, present (Spizellomycetales, Monoblepharidales). Golgi apparatus 0, stacked cisternae; 1, absence of stacked cisternae. Location of Golgi apparatus 0, absent; 1, associated with flagellar rootlet; 3, not associated with flagellar rootlet. Relationship of microbodies to lipid(s) [pending] 0, on lipid; 1, MB with single lipids; 2, MB with multiple lipids; Dissect along ordinal lines. Location of cisterna to lipid(s) [pending] Dissect along ordinal lines. 0, Adjacent to lipids (Chytridiales); 1, Backs microbody (Blastocladiales); 2, Fenestrated cisternae (rumposome) backed by microbody (Monoblepharidales); 3, absent (Batracochytrium, Locustromyces). Location of mitochondria to lipid(s) [pending] proximal to microbodies, keep unresolved; dissect along ordinal lines. Nuclear spur 0, absent; 1, present. Nuclear location 0, distal to kinteosome; 1, proximal to kinetosome; 2, connected to kinetosome. Posterior dome composed of peripheral granules and array of megatubules 0, absent; 1, present. .