Catalog # Aliquot Size P321-911-05 3 x 5 nmol P321-911-20 3 x 20 nmol P321-911-50 3 x 50 nmol PFKFB1 siRNA Set I siRNA duplexes targeted against three exon regions Catalog # P321-911 Lot # Z2073-19 Specificity Formulation PFKFB1 siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human PFKFB1 expression. shipped at room temperature. Product Description Reconstitution Protocol PFKFB1 siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human PFKFB1 mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_002625. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot F6PK; HL2K; PFRX 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or PFKFB1 Protein P321-30G below -20oC. After resuspension, siRNA stock solutions ≥2 PFKFB3 Protein P323-30G µM can undergo up to 50 freeze-thaw cycles without PFKFB4 Protein P324-30G significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Scientific Background PFKFB1 is a member of the family of bifunctional 6- PFKFB1 siRNA Set I phosphofructo-2-kinase: fructose-2, 6-biphosphatase siRNA duplexes targeted against three exon regions enzymes which forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6- Catalog # P321-911 biphosphate using independent catalytic domains (1). Specific Lot # Z2073-19 Fructose-2,6-biphosphate is an activator of the glycolysis Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol pathway and an inhibitor of the gluconeogenesis Format Lyophilized powder pathway. Regulation of fructose-2,6-biphosphate levels Stability 1yr at -70oC from date of shipment through the activity of this enzyme is thought to regulate Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is glucose homeostasis (2). recommended that the lyophilized and resuspended siRNAs are stored at References or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can 1. Algaier, J.et.al: Molecular cloning, sequence analysis, undergo up to 50 freeze-thaw cycles and expression of a human liver cDNA coding for without significant degradation. For fructose-6,2-kinase:fructose-2,6-bisphosphatase. long-term storage, it is recommended that the siRNA is stored at -70oC. For Biochem. Biophys. Res. Commun. 153: 328-333, 1988. most favorable performance, avoid 2. Darville, M. I. et.al: Complete nucleotide sequence repeated handling and multiple coding for rat liver 6-phosphofructo-2- freeze/thaw cycles. kinase/fructose-2,6-bisphosphatase derived from a cDNA clone. FEBS Lett. 224: 317-321, 1987. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .