NFYC Antibody Cat. No.: 58-221 NFYC Antibody Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat This NFYC antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 100-129 amino acids from the N-terminal region of human NFYC. TESTED APPLICATIONS: WB APPLICATIONS: For WB starting dilution is: 1:1000 PREDICTED MOLECULAR 50 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated September 29, 2021 1 https://www.prosci-inc.com/nfyc-antibody-58-221.html PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: NFYC Nuclear transcription factor Y subunit gamma, CAAT box DNA-binding protein subunit C, ALTERNATE NAMES: Nuclear transcription factor Y subunit C, NF-YC, Transactivator HSM-1/2, NFYC ACCESSION NO.: Q13952 PROTEIN GI NO.: 20137773 GENE ID: 4802 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a BACKGROUND: prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Multiple transcript variants encoding different isoforms have been found for this gene. REFERENCES: 1) Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010) 2) Murai-Takeda, A., et al. J. Biol. Chem. 285(11):8084-8093(2010) 3) Ceribelli, M., et al. J. Biol. Chem. 284(49):34189-34200(2009) 4) Yamanaka, K., et al. Genomics 94(4):219-227(2009) ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 29, 2021 2 https://www.prosci-inc.com/nfyc-antibody-58-221.html.