Efficient Professional Protein and Antibody Platforms MAdCAM-1 Antibody Basic information: Catalog No.: UMA60863 Source: Rat Size: 50ul/100ul Clonality: Rat monoclonal Concentration: 1mg/ml Isotype: Rat IgG2a Purification: Immunogen affinity purified Useful Information: WB:1:100-1:1000 IP:1-2ug per 100-500ug of total protein (1ml of cell lysate) Applications: IF:1:50-1:500 FC:1ug per 1*10^6 cells Reactivity: Human, Mouse, Rat Specificity: This antibody recognizes MAdCAM-1 protein. Endothelial cells from BALB/c mouse mesenteric and peripheral lymph Immunogen: nodes. The recirculation of lymphocytes through different organs is thought to be regulated by adhesion molecules (“homing receptors”) recognizing tis- sue-specific vascular addressins on the endothelium. The mucosal vascular addressin, MadCAM-1 (mucosal addressin cell adhesion molecule 1), is an immunoglobulin superfamily adhesion molecule for lymphocytes that is ex- pressed by mucosal venules and helps direct lymphocyte traffic into Peyer’s Description: patches and the intestinal lamina propria. MadCAM-1 acts as an endothelial cell ligand for leukocyte homing receptors L-Selectin and Integrin ┒4/β7. MadCAM-1 is strongly expressed on inflamed portal vein/sinusoidal endo- thelium in autoimmune-mediated liver disease and plays a major contribu- tory role in the progression of chronic experimental autoimmune encepha- lomyelitis. Uniprot: Q13477 Human BiowMW: 40/29 kDa Buffer: 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Western blot analysis of MAdCAM-1 expression in ECV304 whole cell lysate. Western blot analysis of MAdCAM-1 expression in non-transfected (A) and mouse MAdCAM-1 transfected (B) 293T whole cell lysates. Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] .