Anti-USP10 monoclonal antibody, clone 3F2 (DCABH-1085) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Product Overview Mouse monoclonal to USP10 Antigen Description Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Immunogen Recombinant full length Human USP10 produced in HEK293T cells (NP_005144). Isotype IgG1 Source/Host Mouse Species Reactivity Mouse, Rat, Dog, Human, Monkey Clone 3F2 Purity Protein G purified Conjugate Unconjugated Applications WB, IHC-P, Flow Cyt, ICC/IF Positive Control HEK293T cells transfected with pCMV6-ENTRY USP10 cDNA; HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cell extracts; Human breast, pancreas, endometrium, endometrium adenocarcinoma, prostate, bladder carcinoma, lymphoma and tonsil tissues; COS Format Liquid Size 100 μl Buffer pH: 7.30; Preservative: 0.02% Sodium azide; Constituents: 48% PBS, 1% BSA, 50% Glycerol Preservative 0.02% Sodium Azide Storage store at -20°C. Avoid repeated freeze / thaw cycles. Ship Shipped at 4°C. 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved GENE INFORMATION Gene Name USP10 ubiquitin specific peptidase 10 [ Homo sapiens ] Official Symbol USP10 Synonyms USP10; ubiquitin specific peptidase 10; ubiquitin specific protease 10; ubiquitin carboxyl-terminal hydrolase 10; KIAA0190; UBPO; ubiquitin thioesterase 10; deubiquitinating enzyme 10; ubiquitin thiolesterase 10; ubiquitin-specific-processing protease 10; Entrez Gene ID 9100 Protein Refseq NP_005144 UniProt ID Q14694 Chromosome Location 16q Function cysteine-type peptidase activity; cystic fibrosis transmembrane conductance regulator binding; p53 binding; peptidase activity; protein binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity; ubiquitin-specific protease activity; 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.