Thermo EASY-Spray Series Ion Source User Guide 60053-97260 Revision B May 2013 © 2013 Thermo Fisher Scientific Inc. All rights reserved. Acclaim, Accucore, EASY-nLC, EASY-Spray, EASY-Spray NG, Foundation, nanoViper, Optima, PepMap, PepSwift, Q Exactive, Velos Pro, and Viper are trademarks, and Dionex, Exactive, LCQ, LTQ, Orbitrap, Orbitrap Fusion, Thermo Scientific, TSQ Endura, TSQ Quantiva, TSQ Quantum Ultra, UltiMate, and Xcalibur are registered trademarks of Thermo Fisher Scientific Inc. in the United States. The following are registered trademarks in the United States and other countries: Dino-Lite is a registered trademark of AnMo Electronics Corporation. IDEX is a registered trademark of IDEX Health & Science LLC. Microsoft and Windows are registered trademarks of Microsoft Corporation. PEEK is a trademark of Victrex plc. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. 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This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents. Release history: Revision A, May 2012; Revision B, May 2013 For Research Use Only. Not for use in diagnostic procedures. C Contents Preface . xi Compatible Mass Spectrometers . xii Product Requirements . xii Contents of the EASY-Spray Series Ion Source Kits . .xiii Safety and Special Notices . xv Contacting Us . .xvi Chapter 1 Introduction . .1 Advantages of Nanoelectrospray . 2 Functional Description . 3 Source Housing. 3 Controller PCB . 5 Controller Power Supply . 6 Chapter 2 Installing the EASY-Spray Series Ion Source . .7 Installing the Ion Source onto the Mass Spectrometer . 8 Connecting the Controller Power Supply . 11 Configuring the Venting Tee Holder . 12 Adjusting the Emitter Tip Position . 14 Removing the EASY-Spray Series Source. 17 Chapter 3 Connecting the LCD Monitor and Video Camera. .19 Connecting the Monitor and Camera . 19 Adjusting the Video Picture. 21 Chapter 4 Connecting the LC to the EASY-Spray Column . .23 Tools and Supplies . 23 Installing the EASY-Spray Column for the First Time. 25 Using the nanoViper Fitting . 26 Connecting the LC to the EASY-Spray Column . 27 Connecting an EASY-nLC 1000 Instrument to the EASY-Spray Column. 27 Connecting an EASY-nLC II Instrument to the EASY-Spray Column . 31 Connecting an RSLCnano System to the EASY-Spray Column. 33 Thermo Scientific EASY-Spray Series Ion Source User Guide iii Contents Chapter 5 Configuring the Mass Spectrometer for NSI Mode . .37 Configuring the EASY-Spray NG Source’s NSI Parameters. 37 Configuring the EASY-Spray Source’s NSI Parameters . 38 Selecting the Source for the LCQ Deca XP Max Mass Spectrometer . 40 Chapter 6 Maintenance . .41 Guidelines . 41 Preparing the Work Area. 42 Tools and Supplies . 42 Cleaning the EASY-Spray Column Emitter Tip . 44 Replacing the Controller PCB. 45 Chapter 7 Troubleshooting. .47 Chapter 8 Replaceable Parts. .51 Spare Parts. 51 Consumables. 51 Glossary . .53 Index . .55 iv EASY-Spray Series Ion Source User Guide Thermo Scientific F Figures Figure 1. nanoLC/MS system using the EASY-Spray ion source, camera, and monitor . 2 Figure 2. EASY-Spray NG source housing (P/N ES082) . 3 Figure 3. EASY-Spray source housing (P/N ES081) . 4 Figure 4. Controller PCB located on the bottom of the source (P/N ES082) . 5 Figure 5. Controller power supply assembly (shown with a European input plug) . 6 Figure 6. Examples of the mass spectrometer ion sweep cones . 9 Figure 7. EASY-Spray NG locking lever positions (top view) . 9 Figure 8. EASY-Spray locking lever positions (top view) . 10 Figure 9. EASY-Spray NG source housing connection (ES082) . 10 Figure 10. EASY-Spray source housing connection (ES081) . 11 Figure 11. Power connection for the controller PCB (bottom view) . 12 Figure 12. Screw to remove before installing the Tee holder . 13 Figure 13. Tee holder in the shipping configuration . 13 Figure 14. Emitter positioning tool . 14 Figure 15. Gap positions in front of the mass spectrometer’s ion transfer tube (side view) . 14 Figure 16. X- and z-axes position controls on the source (P/N ES081) . 15 Figure 17. Y-axis position control (P/N ES082). 16 Figure 18. Camera and column installed on the EASY-Spray source (P/N ES081) . 20 Figure 19. EASY-Spray column . 25 Figure 20. nanoViper fitting . 26 Figure 21. Recommended plumbing locations on the venting tee (nanoViper example) . 28 Figure 22. Tee holder secured to the bottom of the column holder . 28 Figure 23. Example of a one-column setup with nanoViper fittings. 29 Figure 24. Example of a two-column setup with nanoViper fittings and a Viper union . 30 Figure 25. LC line with sleeve, ferrule, and nut . 31 Figure 26. Column Out line with smaller fitting from the ZDV adapter union . 32 Figure 27. Example of a two-column setup for the EASY-nLC II . 33 Figure 28. Example of a one-column setup for an RSLCnano system (direct injection) . 34 Figure 29. Example of a two-column setup for an RSLCnano system (preconcentration) . 35 Figure 30. Ion Source page in the Tune window for the Orbitrap Fusion (example) . 38 Figure 31. Tune Plus window for an NSI-configured Velos Pro (example) . 39 Figure 32. NSI Source dialog box . 39 Thermo Scientific EASY-Spray Series Ion Source User Guide v Figures Figure 33. Wash cap installed over the column emitter tip . 44 Figure 34. EASY-Spray with the new controller PCB and extra cover plate (bottom view) . 45 Figure 35. Controller PCB (P/N ES233). 45 Figure.