Anti-CSTF1 (C-terminal) produced in rabbit, affinity isolated antibody Product Number C2372 Product Description Reagent Anti-CSTF1 (C-terminal) is produced in rabbit using as Supplied as a solution in 0.01 M phosphate buffered the immunogen a synthetic peptide corresponding to a saline, pH 7.4, containing 15 mM sodium azide as a sequence at the C-terminal of human CSTF1 (GeneID: preservative. 1477) conjugated to KLH. The corresponding sequence is identical in mouse and rat. The antibody is affinity- Antibody concentration: 1.0 mg/mL purified using the immunizing peptide immobilized on agarose. Precautions and Disclaimer For R&D use only. Not for drug, household, or other Anti-CSTF1 (C-terminal) specifically recognizes human uses. Please consult the Safety Data Sheet for CSTF1 (also known as Cleavage stimulation factor, information regarding hazards and safe handling 3 pre-RNA, subunit 1, 50 kDa, CSF-50 subunit). The practices. antibody may be used in several immunochemical techniques including immunoblotting (48 kDa) and Storage/Stability immunoprecipitation. Detection of the CSTF1 band by Store at –20 C. For continuous use, the product may immunoblotting is specifically inhibited with the be stored at 2–8 C for up to one month. For extended immunizing peptide. storage, freeze in working aliquots at –20 C. Repeated freezing and thawing, or storage in “frost-free” freezers, mRNA precursors are processed 3-ends in a two-step is not recommended. If slight turbidity occurs upon reaction; endonucleolytic cleavage at the poly(A) site prolonged storage, clarify the solution by centrifugation followed by the addition of adenylate residues to form a before use. Working dilutions should be discarded if not poly(A) tail. The mammalian mRNA 3 end processing used within 12 hours. complex contains several sub-complexes. These include the cleavage-polyadenylation specificity factor Product Profile (CPSF), which recognizes the nearly ubiquitous Immunoblotting: a working antibody concentration of AAUAAA signal, cleavage stimulatory factor (CstF), 2-4 g/mL is recommended using K562 lysates. which interacts with a less-conserved G/U-rich sequence situated downstream of the cleavage site, Immunoprecipitation: a working antibody amount of cleavage factors I and II (CFI and CFII) and poly(A) 5-10 g is recommended using K562 lysates. polymerase (PAP). Note: In order to obtain best results in various CstF is a heterotrimer consisting of subunits of 50, 64 techniques and preparations, it is recommended to and 77 kDa referred to as CSTF1, CSTF2 and CSTF3 determine optimal working dilutions by titration. respectively. CSTF1 is required for in vitro cleavage activity. It contains seven WD-40 repeats, which were References shown to be required for the interaction with CSTF3. 1. Zhao, J. et al., Microbiol. Mol. Biol. Rev., 63,405- CSTF1 also interacts directly with the RNA polymerase 445 (1998). II CTD, suggesting a role in linking transcription and 2. Mandel, C.R. et al., Cell. Mol. Life Sci., 65, 1099- mRNA 3-ends processing.1-2 1122 (2008). VS,SG,KAA,PHC,MAM 04/19-1 2019 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip. .