2017_SUPPL_2_con indice.qxp_Hrev_master 01/09/17 11:58 Pagina I european journal of histochemistry a journal of functional cytology ISSN 1121-760X volume 61/supplement 2 2017 PROCEEDINGS OF THE 37TH NATIONAL CONGRESS OF THE ITALIAN SOCIETY OF HISTOCHEMISTRY Taormina, September 22-23, 2017 San Domenico Palace Hotel Presidents Giuseppe Anastasi, Domenico Puzzolo Vice Presidents Ludovico Magaudda, Giovanni Tuccari Organizing Commitee Alba Arco, Valeria Barresi, Daniele Bruschetta, Antonio Centofanti, Giuseppina Cutroneo, Debora Di Mauro, Antonio Duca, Angelo Favaloro, Antonio Ieni, Antonio Micali, Alba Migliorato, Demetrio Milardi, Simona Pergolizzi, Antonina Pisani, Maria Righi, Giuseppina Rizzo, Giuseppe Santoro, Fabio Trimarchi, Giovanna Vermiglio Scientific Commitee Salvatore Bertino, Alberto Cacciola, Giorgio Cacciola, Elena Filippini, Grazia Giulia, Pantè Claudia Previti, Cecilia Spoto Scientific Secreteriat Dipartimento Di Scienze Biomediche, Odontoiatrichee delle Immagini Morfologiche d Funzionali ejh Organizing Secreteriat Bisazza Gangi Eventi E Congressi under the auspices of the University of Pavia, Italy Guest Editors Giuseppe Anastasi, Domenico Puzzolo 2017_SUPPL_2_con indice.qxp_Hrev_master 01/09/17 11:58 Pagina II european journal of histochemistry Published by PAGEPress, Pavia, Italy a journal of functional cytology ISSN 1121-760X volume 61/supplement 2 Editorial Office: 2017 Via A. Cavagna Sangiuliani 5, 27100 Pavia, Italy Phone: +39.0382.464340 - Fax: +39.0382.34872 E-mail: [email protected] Printed quarterly by: Press Up s.r.l. Editor in Chief C. Pellicciari via La Spezia, 118/C 00055 - Ladispoli (RM) Phone and Fax: +39.076.15.27.351 Editor M. Biggiogera Annual Subscriptions Dipartimento di Biologia e Biotecnologie Europe: Euro 200 “Lazzaro Spallanzani” Università di Pavia All other Countries: $ 250 Subscriptions, cancellations, business correspondence and any enquiries must be sent to PAGEPress Publications, Pavia, Italy. Cancellations must be received before the end of September to take effect at the end of the same year. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means (electronic, electrostatic, magnetic type, mechanical, photo- copying or otherwise) without written permission by the Publishers. Reg. Tribunale di Pavia n. 289/23.2.1984. Recognized by the Ministero per i Beni e le Attività Culturali, Italy as a publication of high cultural value. Associato all’USPI Unione Stampa Periodica Italiana Disclaimer. Whilst every effort is made by the publishers and the editorial board to see that no inaccurate or misleading data, opinion or statement appears in this journal, they wish to make it clear that the data and opinions appearing in the articles or advertisements herein are the responsibility of the contributor or advisor concerned. Accordingly, the publisher, the editorial board and their respective employees, officers and agents accept no lia- bility whatsoever for the consequences of any inaccurate or mis- leading data, opinion or statement. 2017_SUPPL_2_con indice.qxp_Hrev_master 01/09/17 11:58 Pagina III European Journal of Histochemistry a journal of functional cytology The European Journal of Histochemistry was founded Managing Editors in 1954 by Maffo Vialli and published until 1979 C.A. Redi (University of Pavia, Italy) under the title of Rivista di Istochimica Normale e E. Solcia (University of Pavia, Italy) for Japan: M. Fukuda (Fukui Medical School, Fukui, Patologica, from 1980 to 1990 as Basic and Applied Japan) Histochemistry and in 1991 as European Journal of for Latin America: R.F. Donoso (University of Chile) Basic and Applied Histochemistry. It is published for USA and Canada: A. Nanci (University of under the auspices of the University of Pavia, Italy. Montreal, Canada) The European Journal of Histochemistry is the offi- cial organ of the Italian Society of Histochemistry Assistant Editor and a member of the journal subcommittee of the Manuela Malatesta (University of Verona, Italy) International Federation of Societies for Managing Board of the Italian Society of Histochemistry and Cytochemistry (IFSHC). Histochemistry for the years 2014-2017 Carlo Pellicciari (President), University of Pavia, The Journal publishes Original Papers, Technical Italy Reports, Reviews, Brief Reports, Letters to the Editor, Roberto Di Primio (vice-President), University of Book Reviews, Views and Comments, concerning Ancona, Italy investigations performed with the aid of biophysical, Paolo Onori (Member), University of Rome "La biochemical, molecular-biological, enzymatic, immu - Sapienza", Italy Paola Sirigu (Member), University of Cagliari, Italy nohistochemical, cytometric, and image analysis tech- Silvia Modina (Secretary), University of Milan, Italy niques. Fiorenza De Bernardi (past-President), University of Areas of particular interest to the European Journal Milan, Italy of Histochemistry include: - functional cell and tissue biology in animals and Editorial Board plants; B. Bilinska, Warsaw; E. Bonucci, Rome; G. Bottiroli, - cell differentiation and death; Pavia; L. Cocco, Bologna; A. Diaspro, Genoa; - cell-cell interaction and molecular trafficking; E. Falcieri, Urbino; P. Hozak, Prague; Z. Kmiec, Gdansk; A. Lauria, Milan; H.G. Lee, Cleveland; N.M. - biology of cell development and senescence; Maraldi, Bologna; F.J. Medina, Madrid; G. Meola, - nerve and muscle cell biology; Milan; S. Modina, Milan; S. Muller, Strasbourg; P. - cellular basis of diseases. Panula, Helsinki; M. Pavelka, Vienna; M.T. Perra, Cagliari; G. Perry, San Antonio; G. Rindi, Rome; A. Editor in Chief Riva, Cagliari; C. Sotelo, Paris; P.G. Suh, South Carlo Pellicciari (University of Pavia, Italy) Korea; J.P. Tremblay, Quebec. Editor Editorial Staff Nadia Moscato, Managing Editor Marco Biggiogera (University of Pavia, Italy) Cristiana Poggi, Production Editor Tiziano Taccini, Technical Support 2016 Impact factor: 2.306. ©JCR Clarivate Analytics 2017_SUPPL_2_con indice.qxp_Hrev_master 01/09/17 11:58 Pagina IV 2017_SUPPL_2_con indice.qxp_Hrev_master 01/09/17 11:58 Pagina V ejh table of contents 37TH NATIONAL CONGRESS OF THE ITALIAN SOCIETY OF HISTOCHEMISTRY Opening Lecture................................................................................... 1 Oral Presentations................................................................................2 Index of Authors.................................................................................14 european journal of histochemistry ISSN 1121-760X volume 61/supplement 2 2017 2017_SUPPL_2_con indice.qxp_Hrev_master 01/09/17 11:58 Pagina VI 2017_SUPPL_2_con indice.qxp_Hrev_master 01/09/17 11:58 Pagina 1 European Journal of Histochemistry 2017; vol. 61; supplement 2 Opening Lecture (SCPPP) cluster whose members are implicated in stabilization of calcium and phosphate ions in tissue fluids and regulation of their deposition in extracellular matrices. The three proteins are expressed in maturation stage ameloblasts as well as in the junc- FROM THE STUDY OF ENAMEL TO NEW INSIGHTS ON tional epithelium (JE), the part of the gingiva that seals away EPITHELIAL CANCERS the periodontal tissues from the aggressive oral environment. In A. Nanci both cases, they localize to a specialized basal lamina (sBL) Laboratory for the Study of Calcified Tissues and Biomaterials, that attaches epithelial cells to the mineralized tooth surface. Faculty of Dental Medicine, University of Montréal, Quebec, The knockout of ODAM exhibits a JE phenotype, and its expres- Canada sion is downregulated during periodontal disease. Furthermore, E-mail: [email protected] the major pathogens implicated in periodontal disease can actively degrade ODAM. Strikingly, ODAM, but not the other In order to better understand the process by which enamel two proteins, is also expressed by stem cell clusters in the peri- becomes the hardest extracellular calcified matrix of the body, odontium when periodontal integrity is affected, and by various our group has carried out transcriptomic screens of the rat inci- epithelial neoplasias whose tissues of origin normally do not sor enamel organ. This has led to the discovery of three genes express it. Altogether, these findings suggest that ODAM behaves encoding novel proteins named amelotin (AMTN), odontogenic as a multifunctional matricellular protein that participates both ameloblast-associated (ODAM), and secretory calcium-binding in structuring the aBL and in regulating cell status. phosphoprotein proline-glutamine rich 1 (SCPPPQ1). These genes belong to the secretory calcium-binding phosphoprotein Supported by the CIHR, CRC program, FCI and RSBO-FRQ. 1 2017_SUPPL_2_con indice.qxp_Hrev_master 01/09/17 11:58 Pagina 2 37th National Congress of the Italian Society of Histochemistry, Taormina, September 22-23, 2017 Oral Presentations IMMUNOHISTOCHEMICAL EXPRESSION OF AQUAPOR- IN 1 IN PLEURAL EFFUSIONS FROM MALIGNANT MESOTHELIOMA: AN APPROACH BY CELL-BLOCK PRO- TEMPERATURE EFFECT ON MUSCLE GROWTH IN CEDURE ACIPENSER BAERII YOLK-SAC LARVAE G. Angelico1, A. Ieni1, S. Spadola2, R. Caltabiano2, P. Zeppa3, L. Aidos1, M. Lanfranchi2, C. Domeneghini1, A. Di Giancamillo1 G. Tuccari1 1Department of Health, Animal Science and Food Safety, 1Department of Human Pathology of Adult and Developmental University of Milan; 2Società Naviglio Agricola SS, Goito, Italy Age “Gaetano Barresi”, Unit of Pathological Anatomy, E-mail: [email protected] University of Messina, AOU Policlinico G. Martino; 2Department