(12) United States Patent (10) Patent No.: US 7,998,740 B2 Sackstein (45) Date of Patent: Aug

(12) United States Patent (10) Patent No.: US 7,998,740 B2 Sackstein (45) Date of Patent: Aug

US007998.740B2 (12) United States Patent (10) Patent No.: US 7,998,740 B2 Sackstein (45) Date of Patent: Aug. 16, 2011 (54) CYTOKINE INDUCTION OF SELECTIN Butcher, E. C., "Leukocyte-endothelial cell recognition: three (or LGANDS ON CELLS more) steps to specificity and diversity”. Cell, 67: 1033-6 (1991). Cowlandet al., “Isolation of neutrophil precursors from bone marrow (76) Inventor: Robert Sackstein, Sudbury, MA (US) for biochemical and transcriptional analysis”. J. Immunol. Meth. 232:191-200 (1999). (*) Notice: Subject to any disclaimer, the term of this Dereure et al., “Neutrophil-dependent cutaneous side-effects of patent is extended or adjusted under 35 leucocyte colony-stimulating factors: manifestations of a neutrophil U.S.C. 154(b) by 325 days. recovery syndrome'. Brit. J. Dermatol., 150: 1228-30 (2004). Dimitroff et al., “CD44 is a major E-selectin ligand on human (21) Appl. No.: 11/779,650 hematopoietic progenitor cells'. J. Cell. Biol. 153:1277-86 (2001). Dimitroffet al., “A distinct glycoform of CD44 is an L-selectin ligand (22) Filed: Jul.18, 2007 on human hematopoietic cells'. Proc. Natl. Acad. Sci. USA, (65) Prior Publication Data 97:13841-6 (2000). Dimitroffet al., “Differential L-selectin binding activities of human US 2009/00531.98 A1 Feb. 26, 2009 hematopoietic cell L-selectin ligands, Hcell and PSGL-1”. J. Biol. Chem., 276:47623-31 (2001). Related U.S. Application Data Elfenbein et al., “Primed marrow for autologous and allogeneic trans (60) Provisional application No. 60/831,525, filed on Jul. plantation: a review comparing primed marrow to mobilized blood 18, 2006. and steady-state marrow”. Exp. Hematol. 32:327-39 (2004). Ellies et al., “Sialyltransferase specificity in selectin ligand forma (51) Int. Cl. tion”. Blood, 100:3618-25 (2002). A6 IK38/00 (2006.01) Faulkner et al., “G-CSF serum pharmacokinetics during peripheral CI2N 5/07 (2010.01) blood progenitor cell mobilization: neutrophil count-adjusted dosage CI2N 5/074 (2010.01) might potentially improve mobilization and be more cost-effective'. CI2N 5/078 (2010.01) Bone Marr. Tran. 21:1091-5 (1998). A6 IK3I/35 (2006.01) Fuhlbrigge et al., “Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells', Nature, (52) U.S. Cl. .......... 435/375; 435/377; 514/1.1, 514/7.9; 389:978-81 (1997). 514/451 Fuhlbrigge et al., “Direct real-time observation of E- and P-selectin (58) Field of Classification Search ........... ... None mediated rolling on cutaneous lymphocyte-associated antigen immo See application file for complete search history. bilized on Western blots”, J. Immunol., 168:5645-51 (2002). 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Goetz et al., “Isolated P-selectin glycoprotein ligand-1 dynamic Katayama et al., J Exp Med. Apr. 18, 2005; 201(8): 1183-1189.* adhesion to P- and E-selectin”. J. Cell. Biol. 137:509-19 (1997). Katoh et al., The Journal of Immunology, 1999, 162: 5058-5061.* Groves et al., “Endothelial leucocyte adhesion molecule-1 (ELAM Hakansson, L. et al. Br. J. Haematol. 98, 603-611 (1997).* 1) expression in cutaneous inflammation'. Brit. J. Dermatol., (Cameron, “Cytokines, Chemokines and Their Receptors' Ma 124:117-23 (1991). dame Curie Bioscience Database; access from http://www.ncbi.nlm. Hakansson et al., “Effects of in vivo administration of G-CSF on nih.gov/bookshelf brfcgi?book=eurekah&part=A13503 on Mar, 4. neutrophil and eosinophil adhesion', Brit. J. Haematol. 98:603-11 2010.* (1997). McKinstry et al., (Blood. Jan. 1, 1997:89(1):65-71).* Adler et al., “Fatal sickle cell crisis after granulocyte colony-stimu (Continued) lating factor administration”. Blood,97: 3313-33149 (2001). Albert, R. K., “Mechanisms of the adult respiratory distress syn drome: selectins”. Thorax. 50 (Suppl 1):S49-52 (1995). Primary Examiner — Jeffrey Stucker Arimura et al., “Acute lung Injury in a healthy donor during mobili Assistant Examiner — Daniel C Gamett Zation of peripheral blood stem cells using granulocyte-colony stimu lating factor alone'. Haematologica 90, ECR10 (2005). (74) Attorney, Agent, or Firm — Mintz Levin Cohn Ferris Azoulay et al., “Exacerbation by granulocyte colony-stimulating fac Glovsky and Popeo, P.C.: Cynthia A. Kozakiewicz, J.D. tor of prior acute lung injury: implication of neutrophils'. Crit. Care Med., 30:21 15-22 (2002). Azoulay et al., “Granulocyte colony-stimulating factor or neutrophil (57) ABSTRACT induced pulmonary toxicity: myth or reality? Systematic Review of Clinical Case Report Experimental Data'. Chest, 120: 1695-701 Methods and compositions for treating cells with cytokines (2001). are provided herein. 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J. Physiol. Cell. Physiol. (2005). controversies”. Blood, 88:3259-87 (1996). Wakasugi et al., “Two Distinct Cytokines Released from a Human Kieffer et al., “Neutrophils, monocytes, and dendritic cells express Aminoacyl-tRNA Synthesis”, Sci., 284(2): 147 (1999). the same specialized form of PSGL-1 as do skin-homing memory T Li et al., “CD3-and CD28-Dependent Induction of PDE7 Required cells: cutaneous lymphocyte antigen'. Biochem. Biophys. Res. Com for T Cell Activation”, Sci., 283:848 (1999). mun., 285:577-87 (2001). Springer, Timothy A., “Traffic Signals for Lymphocyte Recirculation Kriegsmann et al., “Expression of E-selectin messenger RNA and and Leukocyte Emigration: The Multistep Paradigm'. Cell, 76:301 protein in rheumatoid arthritis'. Arth. Rheum., 38:750-4 (1995). 314 (1994). Kunkel et al., “Distinct phenotype of E-selectin-deficient mice. Sipkins et al., “In Vivo Imaging of Specialized Bone Marrow E-selectin is required for slow leukocyte rolling in vivo”. Circ. 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