IFITM1 Antibody Cat. No.: 5807 IFITM1 Antibody Immunofluorescence of IFITM1 in Jurkat cells with IFITM1 Immunocytochemistry of IFITM1 in Jurkat cells with IFITM1 antibody at 20 μg/mL. antibody at 20 μg/mL. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat IFITM1 antibody was raised against a 16 amino acid synthetic peptide near the center of human IFITM1. IMMUNOGEN: The immunogen is located within amino acids 40 - 90 of IFITM1. TESTED APPLICATIONS: ELISA, ICC, IF, WB September 23, 2021 1 https://www.prosci-inc.com/ifitm1-antibody-5807.html IFITM1 antibody can be used for detection of IFITM1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS: Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. POSITIVE CONTROL: 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate 2) Cat. No. 17-005 - Jurkat Cell Slide Predicted: 14 kDa PREDICTED MOLECULAR WEIGHT: Observed: 14 kDa Properties PURIFICATION: IFITM1 Antibody is affinity chromatography purified via peptide column. CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: IFITM1 Antibody is supplied in PBS containing 0.02% sodium azide. CONCENTRATION: 1 mg/mL IFITM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: IFITM1 IFITM1 Antibody: 9-27, CD225, IFI17, LEU13, DSPA2a, Interferon-induced transmembrane ALTERNATE NAMES: protein 1, Dispanin subfamily A member 2a ACCESSION NO.: NP_003632 PROTEIN GI NO.: 150010589 GENE ID: 8519 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References September 23, 2021 2 https://www.prosci-inc.com/ifitm1-antibody-5807.html IFITM1 Antibody: IFITM1 (Interferon inducible transmembrane protein 1) is a member of the IFN-inducible transmembrane protein family. It is an essential mediator of interferon- gamma-induced antiproliferation and plays a role in the control of cell growth. IFITM1 is upregulated in several tumor types and may be useful as a tumor biomarker. Both mouse BACKGROUND: IFITM1 and IFITM3 are expressed on the cell surfaces of primordial germ cells in a developmentally-regulated manner. IFITM1 activity is required for primordial germ cell transit, and IFITM1 acts as a repulsive molecule by repelling non-IFITM1-expressing primordial germ cells from the mesoderm into the endoderm. 1) Deblandre GA, Marinx OP, Evans SS, et al. Expression cloning of an interferon-inducible REFERENCES: 17 kDa membrane protein implicated in the control of cell growth. J. Biol. Chem. 1995; 270:23860-6. 2) Yang G, Xu Y, Chen X, et al. IFITM1 plays an essential role in the antiproliferative action of interferon-gamma. Oncogene. 2007; 26:594-603. 3) Akyerli CB, Beksac M, Holko M, et al. Expression of IFITM1 in chronic myeloid leukemia patients. Leuk. Res. 2005; 29:283-6. 4) Tanaka SS, Yamaguchi YL, Tsoi B, et al. IFITM/Mil/Fragilis family proteins IFITM1 and IFITM3 play distinct roles in mouse primordial germ cell homing and repulsion. Cell 2005; 9:745-6. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 23, 2021 3 https://www.prosci-inc.com/ifitm1-antibody-5807.html.