V3 – 11/08/11 Sabouraud + Actidione 355-6559 Chloramphenicol/Agar DEFINITION Trichophyton mentagrophytes; Sabouraud agar with the addition of actidione Trichophyton rubrum; and chloramphenicol is recommended for the Epidermophyton flocosum etc. isolation of Dermatophytes and other pathogenic fungi from heavily-contaminated However, this medium does not allow the specimens. isolation of Cryptococcus neoformans or Monosporium apiospermmum . PRESENTATION • Ready-to-use Chloramphenicol inhibits most bacterial 100 ml x 6 bottles code 355-6559 contaminants. PERFORMANCES / QUALITY CONTROL OF THEORETICAL FORMULA THE TEST Peptone 10 g The growth performances of the media are Glucose 20 g verified with the following strains: Actidione (cycloheximide) 0.5 g Performance 24-48H Chloramphenicol 0.5 g STRAINS Agar 15 g at 30-35°C Distilled water 1,000 ml Candida albicans Good growth, white Final pH (25°C) = 6. 0 ± 0.2 ATCC 26790 Candida tropicalis STORAGE Inhibited • Ready-to-use: + 2°C to 25 °C ATCC 750 • Expiration date and batch number are shown on the package. Candida glabrata Inhibited PROTOCOL 7 days at 30-35°C and Inoculation and incubation STRAINS Proceed to isolate of the specimen to be 7 days at 20-25°C analyzed or its decimal dilutions on the Sabouraud + Actidione + Chloramphenicol Good growth downy, Trichophyton rubrum agar. Incubate at 32°C for 3-7 days back red-brown PRECAUTIONS Trichophyton Good growth Comply with Good Laboratory Practice. violaceum Violet pigment UTILISATION Epidermophyton Good growth, powdery, Actidione inhibits the development of certain floccosum brown back light brown fungi ( Candida krusei. Candida tropicalis, Fusarium, aspergillus fumigatus , etc) and has Good growth, downy, Microsporum canis no action on the following pathogenic fungi, back yellow-orange which can be isolated on this medium: All Dermatophytes; Fusarium Inhibited All Candida (except for C. tropicalis, C. krusei, C. pelliculosa ); Sporotrichum schenckii; Histoplasma capsulatum; Blastomyces dermatisis; Coccidioides immitis; Geotrichum candidum; 1/2 V3 – 11/08/11 Sabouraud + Actidione Chloramphenicol/Agar 24-48H at STRAINS 35-38°C Staphylococcus aureus Inhibited ATCC 25923 Pseudomonas aeruginosa Inhibited ATCC 27853 QUALITY CONTROL OF MANUFACTURER Every product manufactured and marketed by Bio-Rad is subject to a quality-assurance procedure at all stages, from the reception of raw materials to the marketing of the end- product. Each batch of finished product undergoes quality control and is marketed only if it satisfies the acceptability criteria. Documentation relative to the production and control of each batch is kept on file. KEY WORDS Sabouraud + actidione + chloramphenicol Dermatophytes / Contaminated specimens / Medium. 2/2 .