Contents Greetings ………………………………………..…… P. 1 Information about the Symposium ………………… P. 2 Symposium Program Amphibian Genomics and Genome Editing …….. P. 4 Amphibian Conservation ………………….....….. P. 5 Poster Presentations ………………………….….. P. 6 Abstracts Lectures Amphibian Genomics and Genome Editing …. P. 11 Amphibian Conservation …………………….. P. 21 Poster Presentations ………………………….….... P. 35 List of Participants …………………….…….………. P. 66 Access ……...………………………………………….. P. 68 Greetings On behalf of the organizing committee, I would like to welcome you to the Institute for Amphibian Biology of Hiroshima University’s International Symposium, “Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing,” held in Hiroshima on March 27–28. The Institute is now engaged in a research project titled “Pioneering amphibian research: conservation of endangered amphibian species and development of gene targeting methods,” which is funded by a special education and research expense from the Japanese Ministry of Education, Culture, Sports, Science and Technology. The project will conclude at the end of fiscal 2013. The purpose of this symposium is to introduce the outcomes and findings of the project, as well as promote international exchange among researchers of amphibian biology. The symposium’s scientific program comprises oral and poster presentations covering research areas such as endangered species conservation, landscape genetics, genome editing, and other topics in amphibian biology. To explore the recent progress in these areas, 10 invited biologists and 4 members of the Institute’s staff will give oral presentations with chaired discussion. In addition, 46 participants will give poster presentations with free discussion. The oral presentations will focus on genome editing on March 27 and on endangered species conservation on March 28. I hope that this symposium is able to greatly benefit the study of amphibian biology by inspiring future investigations, stimulating collaborative endeavors, and establishing new friendships. The organizing committee wishes all participants a productive and enjoyable visit, and I look forward to seeing you in Hiroshima. Masayuki Sumida (Ph. D.) Professor and Director Institute for Amphibian Biology, Graduate school of Science, Hiroshima University Chairperson of the Organizing Committee of IABHU International Symposium 1 Information about the Symposium Date 27th (Thu) and 28th (Fri) March 2014 Venue Rooms E002 (Oral presentations) and E203 (Poster presentations), Building E, Graduate School of Science, Hiroshima University, Higashi-Hiroshima Campus 1-3-1 Kagamiyama, Higashi-Hiroshima Hiroshima 739-8526, Japan Schedule (Please see the back cover for details) 27th March Lectures by invited speakers and institute members Poster presentations Backyard tour of the Institute (pre-registration required) Social gathering (pre-registration required) 28th March Lectures by invited speakers and institute members Poster presentations Meeting Fee Participation (including backyard tour) Free Social gathering 5,000 yen (Please pay at symposium check-in) Poster Presentation Maximum poster size is 80 cm (width) × 160 cm (height). Posters should be set up by 14:40 on 27th March, and be removed by 17:30 on 28th March by poster presenters Taking Ceremonial Photographs Group photographs of all of the symposium attendances would be taken near the Building E (the symposium venue). Please attention to announce after the third lecture on 27 March (– 12:10). Backyard Tour of the Institute for Amphibian Biology (pre-registration required) After taking ceremonial photographs, from 12:25 on 27 March, the backyard tour will start. The symposium staff leads the tour attendances to the Institute for Amphibian Biology after taking the ceremonial photo. Lunch The symposium attendances can take lunches in the buffet (open: 8:00 – 19:30) and restaurant (11:00 – 15:00) or buy their lunches in the shop (10:00 – 17:30) that are located in the North Welfare Center No. 1 (please see the campus map: in the last page) close to the Building E (the symposium venue). Social Gathering (pre-registration required) The social gathering will be held in the Saijo Hakuwa Hotel. After completion of the final lecture on 27 March (18:05), the symposium staff leads the participants to this hotel. A courtesy bus that gets off the hotel at 20:40 takes attendances to stations and several major hotels in Higashi-Hiroshima. 2 Contact Address and Correspondence The Office of IABHU International Meeting, c/o Masayuki Sumida Institute for Amphibian Biology Graduate School of Science, Hiroshima University 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8526, Japan E-mail: [email protected] Organizing Committee: Masayuki Sumida (Chairperson), Yoshio Yaoita, Atsushi, Kurabayashi (Chair of Secretariat), Keisuke Nakajima, Ichiro Tazawa, Takeshi Igawa, Islam Mohammed Mafizul, Institute for Amphibian Biology, Graduate School of Science, Hiroshima University, Japan 3 Symposium Program Amphibian Genomics and Genome Editing 27th March Keisuke Nakajima (et. one coauthor) [Lecture by IABHU Member 1] 9:45 - 10:30 The exploitation of genome editing in Xenopus tropicalis Enrique Amaya [Invited Lecture 1] 10:30 – 11:15 Will Xenopus remain an important model organism for biomedical research? Hui Zhao [Invited Lecture 2] 11:15 – 12:10 Efficient targeted gene disruption in Xenopus embryos using TALEN and Crispr/Cas9 systems Takuya Nakayama (et. 5 coauthors) [Invited Lecture 3] 15:40 – 16:25 Xenopus tropicalis, a model organism for the new genetics era: from forward to reverse genetics and now to gene targeting Hajime Ogino [Invited Lecture 4] 16:25 – 17:10 Evolution of a tissue-specific silencer underlies divergence in the expression of paralogues Masanori Taira [Invited Lecture 5] 17:20 – 18:05 The Xenopus laevis genome project: The consequence of allotetraploidization 50 million years ago. 4 Amphibian Conservation 28th March Mohammed Mafizul Islam [Lecture by IABHU Member 2] 9:00 - 9:35 An ex situ conservation effort for several endangered and near-threaten amphibian species from the Ryukyu Archipelago, Japan: Captive breeding of nine anuran species and cryopreservation of sperm for two of them Takeshi Igawa [Lecture by IABHU Member 3] 9:35 - 10:10 Conservation genetics of endangered amphibians in Ryukyu Archipelago towards sustainable conservation of the biodiversity hotspot of amphibians Miguel Vences [Invited Lecture 6] 10:20 – 11:05 Challenges to conserve a megadiverse amphibian fauna in Madagascar, one of the poorest countries of the World Masafumi Matsui [Invited Lecture 7] 11:05 – 11:50 Current Status of Japanese Amphibians and Their Conservation Kelly Zamudio [Invited Lecture 8] 13:50 – 14:35 Ecological and Evolutionary dynamics of the amphibian-killing fungus Robert Jehle [Invited Lecture 9] 14:35 – 15:20 Conservation genetics in amphibians: integrating spatial and temporal population processes Koichi Goka [Invited Lecture 10] 15:30 – 16:15 The Diversity and Endemism of the Chytrid Fungus in Japan Masayuki Sumida [Lecture by IABHU Member 4] 16:15 – 16:50 A case study of fauna conservation in Japan: Current conservation measures of wild populations, captive breeding, genetic diversity, and potential resource materials in the endangered frog species Odorrana ishikawae and O. splendida 5 Poster Presentations (Room E203) P-01 Artificial Breeding of an Indian Bullfrog (Hoplobatrachus tigerinus): A Case Study on Reproduction and Conservation *Mohammad Shafiqul Alam, Md. Mukhlesur Rahman Khan, Md. Mafizul Islam, Mahmudul Hasan, and Masayuki Sumida P-02 Surviving chytridiomycosis: Differential anti-Batrachochytrium dendrobatidis activity inbacterial isolates from three lowland species of Atelopus frogs from Colombia, South America *Sandra V. Flechas, Carolina Sarmiento, Edgar Medina, Martha Cárdenas, Silvia Restrepo, and Adolfo Amézquita P-03 If there’s a silver bullet we can’t find it: options for repatriating chytrid susceptible frogs to the wild are fast evaporating – could genetic modification be our best hope? *Gerry Marantelli, David Hunter, Michael McFadden, Erika Marantelli, Lee Berger, Stephanie Versteegen, and Laura Brannelly P-04 Battle strategies for a chytrid susceptible species: lessons learnt from ten years sending captive bred Spotted Tree Frogs Litoria spenceri to the frontline *Stephanie Versteegen, *Erika Marantelli, Gerry Marantelli, David Hunter, Matt West, and Glen Johnson P-05 Conservation of the Japanese giant salamander Andrias japonicus at Toyosaka-cho, Higashi-Hiroshima, Japan Norio Shimizu, Hiromi Yamasaki, Chika Hashimoto, Kenta Tsuchioka, Tetsuo Takamatsu, Susumu Ueda, Katsunori Sato, Kanji Kato, and *Kazushi Kuwabara P-06 Understanding the origin to preserve in the future: Conservation of Hylid tree frogs in Mesoamerica using an integrative approach *Liliana Solano, Javier Sunyer, and Catherine Walton P-07 Using advanced molecular techniques in a non-model frog: Lessons for amphibian conservation *Heidi K. Smith-Parker, John B. Wallingford, and Michael J. Ryan P-08 Fine-scale case study of detecting genetic structure and gene flow restriction of Japanese brown frog caused by recent urbanization. *Soh Kobayashi, Seiya Abe, and Rikyu Matsuki P-09 Microhabitats of Forest Frogs along Molawin Creek, Mt. Makiling Forest Reserve, Philippines Cabreros, Julie Ann, *de Guzman, Christmas, Corales, Valerie, Molina, Kyla, Moralina, Melchor Jr., Ruiz, Dennis, and Afuang, Leticia 6 P-10 Large scale environmental DNA assessment for Japanese