PREPUBLICATION DRAFT – UNCORRECTED PROOFS Guide for the Care and Use of Laboratory Animals PREPUBLICATION DRAFT Committee for the Update of the Guide for the Care and Use of Laboratory Animals Institute for Laboratory Animal Research Division on Earth and Life Studies THE NATIONAL ACADEMIES PRESS Washington, D.C. www.nap.edu THE NATIONAL ACADEMIES PRESS 500 Fifth Street, NW Washington, DC 20001 NOTICE: The project that is the subject of this report was approved by the Governing Board of the National Research Council, whose members are drawn from the councils of the National Academy of Sciences, the National Academy of Engineering, and the Institute of Medicine. The members of the Committee responsible for the report were chosen for their special competences and with regard for appropriate balance. This study was supported by the Office of Extramural Research, Office of the Director, National Institutes of Health/Department of Health and Human Services under Contract Number N01-OD-4-2139 Task Order# 188; the Office of Research Integrity, Department of Health and Human Services; the Animal and Plant Health Inspection Service, U.S. Department of Agriculture; Association for Assessment and Accreditation of Laboratory Animal Care International; American Association for Laboratory Animal Science; Abbott Fund; Pfizer; American College of Laboratory Animal Medicine; American Society of Laboratory Animal Practitioners; Association of Primate Veternarians. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the organizations or agencies that provided support for the project. 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Upon the authority of the charter granted to it by the Congress in 1863, the Academy has a mandate that requires it to advise the federal government on scientific and technical matters. Dr. Ralph J. Cicerone is president of the National Academy of Sciences. The National Academy of Engineering was established in 1964, under the charter of the National Academy of Sciences, as a parallel organization of outstanding engineers. It is autonomous in its administration and in the selection of its members, sharing with the National Academy of Sciences the responsibility for advising the federal government. The National Academy of Engineering also sponsors engineering programs aimed at meeting national needs, encourages education and research, and recognizes the superior achievements of engineers. Dr. Charles Vest is president of the National Academy of Engineering. The Institute of Medicine was established in 1970 by the National Academy of Sciences to secure the services of eminent members of appropriate professions in the examination of policy matters pertaining to the health of the public. The Institute acts under the responsibility given to the National Academy of Sciences by its congressional charter to be an adviser to the federal government and, upon its own initiative, to identify issues of medical care, research, and education. Dr. Harvey V. Fineberg is president of the Institute of Medicine. The National Research Council was organized by the National Academy of Sciences in 1916 to associate the broad community of science and technology with the Academy’s purposes of furthering knowledge and advising the federal government. Functioning in accordance with general policies determined by the Academy, the Council has become the principal operating agency of both the National Academy of Sciences and the National Academy of Engineering in providing services to the government, the public, and the scientific and engineering communities. The Council is administered jointly by both Academies and the Institute of Medicine. Dr. Ralph J. Cicerone and Dr. Charles M. Vest are chair and vice chair, respectively, of the National Research Council. www.national-academies.org PREPUBLICATION COPY COMMITTEE FOR THE UPDATE OF THE GUIDE FOR THE CARE AND USE OF LABORATORY ANIMALS Members Janet C. Garber (Chair), Garber Consulting R. Wayne Barbee, Virginia Commonwealth University Joseph T. Bielitzki, University of Central Florida Leigh Ann Clayton, National Aquarium, Baltimore John C. Donovan, BioResources, Inc. Coenraad F.M. Hendriksen, Netherlands Vaccine Institute, The Netherlands (until March 2009) Dennis F. Kohn, Columbia University (retired) Neil S. Lipman, Memorial Sloan-Kettering Cancer Center and Weill Cornell Medical College Paul A. Locke, Johns Hopkins Bloomberg School of Public Health The Honorable John Melcher, U.S. Senate (retired) Fred W. Quimby, Rockefeller University (retired) Patricia V. Turner, University of Guelph, Canada Geoffrey A. Wood, University of Guelph, Canada Hanno Würbel, Justus Liebig University of Giessen, Germany Staff Lida Anestidou, Study Director Joanne Zurlo, Director (until April 2010) Kathleen Beil, Administrative Coordinator Cameron H. Fletcher, Editor Rhonda Haycraft, Senior Project Assistant v PREPUBLICATION COPY INSTITUTE FOR LABORATORY ANIMAL RESEARCH COUNCIL Members: Stephen W. Barthold (Chair), Center for Comparative Medicine, University of California, Davis, California Kathryn A. Bayne, Association for Assessment and Accreditation of Laboratory Animal Care International, Frederick, Maryland Myrtle A. Davis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland Jeffrey I. Everitt, Comparative Medicine and Investigator Support, GlaxoSmithKline Research and Development, Research Triangle Park, North Carolina James G. Fox, Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts Nelson L. Garnett, Division of Laboratory Animal Resources, The University of North Carolina, Chapel Hill, North Carolina Estelle B. Gauda, Johns Hopkins University School of Medicine, Johns Hopkins Hospital, Baltimore, Maryland Joseph W. Kemnitz, Wisconsin National Primate Research Center, University of Wisconsin, Madison, Wisconsin Judy A. MacArthur Clark, Animals in Scientific Procedures Inspectorate, Home Office, London, United Kingdom Martha K. McClintock, Institute for Mind and Biology, The University of Chicago, Chicago, Illinois Leticia V. Medina, Animal Welfare and Compliance, Abbott Laboratories, Abbott Park, Illinois Timo Olavi Nevalainen, National Laboratory Animal Center, University of Eastern Finland, Kuopio, Finland Bernard E. Rollin, Department of Animal Sciences, Colorado State University, Fort Collins, Colorado Abigail L. Smith, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania Stephen A. Smith, Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia James E. Womack, Department of Veterinary Pathology, Texas A&M University, College Station, Texas Staff Joanne Zurlo, Director (until April 2010) Lida Anestidou, Senior Program Officer Kathleen Beil, Administrative Coordinator PREPUBLICATION COPY vi Cameron Fletcher, Managing Editor, ILAR Journal Rhonda Haycraft, Program Associate vii PREPUBLICATION COPY INSTITUTE FOR LABORATORY ANIMAL RESEARCH PUBLICATIONS Recognition and Alleviation of Pain in Laboratory Animals (2009) Scientific and Humane Issues in the Use of Random Source Dogs and Cats for Research (2009) Recognition and Alleviation of Distress in Laboratory Animals (2008) Toxicity Testing in the 21st Century: A Vision and a Strategy (2007) Overcoming Challenges to Develop Countermeasures Against Aerosolized Bioterrorism Agents: Appropriate Use of Animal Models (2006) Guidelines for the Humane Transportation of Research Animals (2006) Science, Medicine, and Animals: Teacher's Guide (2005) Animal Care and Management at the National Zoo: Final Report (2005) Science, Medicine, and Animals (2004) The Development of Science-based Guidelines for Laboratory Animal Care: Proceedings of the November 2003 International Workshop (2004) Animal Care and Management at the National Zoo: Interim Report (2004) National Need and Priorities for Veterinarians in Biomedical Research (2004) Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research (2003) International Perspectives: The Future of Nonhuman Primate Resources, Proceedings of the Workshop Held April 17-19, 2002 (2003) Occupational Health and Safety in the Care and Use of Nonhuman Primates (2003) Definition of Pain and Distress and Reporting Requirements for Laboratory Animals: Proceedings of the Workshop Held June 22, 2000 (2000) Strategies That Influence Cost Containment in Animal Research Facilities (2000) Microbial Status and Genetic Evaluation of Mice and Rats: Proceedings of the 1999 US/Japan Conference (2000) Microbial and Phenotypic Definition