Product datasheet Anti-LSM5 Antibody Catalog Number: A10199-2 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Basic Information Product Name Anti-LSM5 Antibody Gene Name LSM5 Source Rabbit IgG Species Reactivity human, mouse Tested Application WB,IHC-P,ICC/IF,FCM,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human LSM5 recombinant protein (Position: N8-V91). Purification Immunogen affinity purified. Observed MW 12KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry in paraffin section IHC-(P)： 1:50-400 Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000 Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing Background Information U6 snRNA-associated Sm-like protein LSm5 is a protein that in humans is encoded by the LSM5 gene. Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. Reference Anti-LSM5 Antibody被引用在0文献中。 暂无引用 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-LSM5 Antibody Catalog Number: A10199-2 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Selected Validation Data Figure 1. Western blot Figure 2. IHC analysis analysis of anti- LSM5 using anti- LSM5 antibody (A10199-2). The antibody (A10199-2). sample well of each lane detected in paraffin- was loaded with 50ug of embedded section of rat sample under reducing liver tissue. Biotinylated conditions. Lane 1: goat anti-rabbit IgG was human HL-60 whole cell used as secondary lysates, Lane 2: human antibody. The tissue K562 whole cell lysates, section was developed Lane 3: human MCF-7 using Strepavidin-Biotin- whole cell lysates. Use Complex (SABC) (Catalog rabbit anti- LSM5 1:1000, # SA1022) with DAB as probed with a goat anti- the chromogen. rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for LSM5 at approximately 12KD. The expected band size for LSM5 at 12KD. Figure 3. IHC analysis Figure 4. IHC analysis using anti- LSM5 using anti- LSM5 antibody (A10199-2). antibody (A10199-2). detected in paraffin- detected in paraffin- embedded section of embedded section of human appendicitis human rectal cancer tissue. Biotinylated goat tissue. Biotinylated goat anti-rabbit IgG was used anti-rabbit IgG was used as secondary antibody. as secondary antibody. The tissue section was The tissue section was developed using Strepavi developed using Strepavi din-Biotin-Complex din-Biotin-Complex (SABC) (Catalog # (SABC) (Catalog # SA1022) with DAB as the SA1022) with DAB as the chromogen. chromogen. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Powered by TCPDF (www.tcpdf.org).