Epigenetics and Cellular Microbiology Lab Micalis Institute, INRAE Jouy-en-Josas, France Post-doctoral or engineer positions: Role of secreted bacterial effectors in the regulation of host RNA splicing and RNA metabolism A post-doctoral or engineer position (depending on the applications received) is available from March 2021 in our research group “Epigenetics and Cellular Microbiology” at the Micalis Institute (a mixed research unit INRAE- AgroParisTech-Paris-Saclay University from the Microbiology Department of the INRAE). Our lab is located in Jouy-en-Josas, in the Paris area (20 km south-west of Paris and 5 km east of Versailles). Scientific environment. We are an international and collaborative team interested in understanding how bacterial infection impacts host cell physiology with a special focus on RNA metabolism. We address novel and original questions in the field of host-pathogen interaction by using L. monocytogenes as bacterial model. We work in a dynamic atmosphere where collaborations and initiatives are welcome. Background and context. Pathogenic bacteria secrete protein effectors called virulence factors to hijack cellular pathways to their own benefit. Almost all of effectors characterized thus far act on host proteins or DNA. Bacterial effectors able to target host RNA have not been reported. We have solid evidence that L. monocytogenes targets host RNA and splicing machinery via secreted virulence factors. We want to elucidate this novel facet of host- pathogen interaction and therefore pave the way for a new field of research focused on bacterial-mediated regulation of RNA Mission. The position is available within the context of a recently obtained ANR grant. By using a combination of molecular and cellular approaches the successful candidate will develop a research project aiming to characterize the effect of one secreted bacterial effector in the regulation of host RNA splicing. The candidate will characterize the host RNA interacting partners of this bacterial effector and the relevance of this interaction in the context of L. monocytogenes infection. Candidate Profile We are looking for a highly driven, motivated and enthusiastic candidate with the following qualifications: - PhD required - Strong experience in RNA biology and molecular biology techniques is mandatory - Ability to work independently - Motivated to learn and develop techniques in new settings - Good communication skills and team spirit Information - ANR-funded 2 years position starting from 1st March 2021. Application deadline 28th February 2021 - Please send your CV and at least two letters of recommendation to [email protected] .