OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] AR09820PU-N Human Adenylate kinase 4 / AK4 (1-223, His-tag) - Purified Alternate names: AK3, AK3L1, ATP-AMP transphosphorylase, Adenylate kinase 3-like Quantity: 0.1 mg Concentration: 1.0 mg/ml (determined by Bradford assay) Background: AK4 (adenylate kinase 4) is a member of the adenylate kinase family of enzymes. This protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Inherited mutations leading to AK4 deficiencies in erythrocytes have been implicated in hemolytic anemia. Uniprot ID: P27144 NCBI: NP_001005353 GeneID: 205 Species: Human Source: E. coli Format: State: Liquid purified protein Purity: >90% Buffer System: 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl Description: Recombinant human AK4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASKLLRAVI LGPPGSGKGT VCQRIAQNFG LQHLSSGHFL RENIKASTEV GEMAKQYIEK SLLVPDHVIT RLMMSELENR RGQHWLLDGF PRTLGQAEAL DKICEVDLVI SLNIPFETLK DRLSRRWIHP PSGRVYNLDF NPPHVHGIDD VTGEPLVQQE DDKPEAVAAR LRQYKDVAKP VIELYKSRGV LHQFSGTETN KIWPYVYTLF SNKITPIQSK EAY Molecular weight: 27.4 kDa (243aa), confirmed by MALDI-TOF (Molecular size on SDS- PAGE will appear higher). Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: Noma T., et al. (2001) Biochem J. 358(1):225-32. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MP/20130116 1 / 2 AR09820PU-N: Human Adenylate kinase 4 / AK4 (1-223, His-tag) - Purified Pictures: Recombinant human AK4, 1-223 aa, His- tagged For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MP/20130116 2 / 2 Powered by TCPDF (www.tcpdf.org).