The following appendix accompanies the article Genetic diversity of picoeukaryotes in a semi-enclosed harbour in the subtropical western Pacific Ocean Man Kit Cheung, Ka Hou Chu, Chi Pang Li, Hoi Shan Kwan, Chong Kim Wong* Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China *Corresponding author. Email: [email protected] Aquatic Microbial Ecology 53:295–305 (2008) Table A1. List of all the operational taxonomic units (OTUs) recovered in the current study. For each OTU, its GenBank closest relative with accession number, the sequence percent similarity, BLAST score, Query/Subject ratio and frequency from each clone library are provided. The remaining columns indicate the OTU frequency from each clone library OTUs OTU frequency Library Clone Closest relative GenBank % similarity BLAST Query/ MB01 MB04 MB07 MB10 TH01 TH04 TH07 TH10 Accession # score Subject Alveolates group II MB01 3 Uncultured marine alveolate group II clone DH147-EKD16 AF290071 94.9 1210 751/791 25 1 7 11 Amoebophrya sp. AF239260 97.6 1279 730/748 1 6 16 Uncultured marine picoplankton AP-picoclone15 DQ386751 94.8 1232 754/795 5 2 4 15 19 Uncultured alveolate clone RA010613.44 AY295708 96.1 861 522/543 1 42 Amoebophrya sp. ex Scrippsiella sp. AF472555 91.9 1096 733/798 2 1 52 Uncultured marine eukaryote clone UEPACAFp5 AY129057 96.6 1319 770/797 8 3 TH01 1 Uncultured marine eukaryote clone UEPACCp3 AY129036 98.9 1317 745/753 4 4 1 8 8 Uncultured marine eukaryote clone UEPACBp4 AY129045 98.8 1330 738/747 3 35 Uncultured eukaryote clone ENI40076.00788 AY937888 94.1 678 430/457 2 2 37 Amoebophrya sp. ex Gymnodinium instriatum AF472554 94.2 1003 629/668 16 42 Uncultured marine picoplankton clone He000803_31 AJ965140 96.3 660 388/403 1 1 MB04 24 Uncultured marine eukaryote clone ENVP10203.00015 DQ917938 99.4 965 528/531 2 25 Amoebophrya sp. ex Scrippsiella sp. AF472555 97.8 1199 680/695 3 TH04 14 Amoebophrya sp. ex Karlodinium micrum AF472553 95 1194 727/765 5 18 Uncultured marine eukaryote clone NOR26.14r DQ119913 99.7 1280 697/699 2 26 Uncultured marine eukaryote clone NOR50.19 DQ119916 90.5 839 589/651 2 MB07 9 Uncultured marine eukaryote clone UEPAC41p3 AY129042 99.6 1400 765/768 1 17 4 21 15 Uncultured eukaryote clone SCM37C36 AY664989 93.2 1031 662/711 1 21 Eukaryote clone OLI11009 AJ402348 98.2 1323 765/779 4 29 Uncultured marine picoplankton AP-picoclone20 DQ386756 98.8 1345 747/756 2 42 Uncultured marine eukaryote clone UEPACEp3 AY129038 98.9 1434 796/805 3 1 Table A1 (continued) OTUs OTU frequency Library Clone Closest relative GenBank % similarity BLAST Query/ MB01 MB04 MB07 MB10 TH01 TH04 TH07 TH10 Accession # score Subject TH07 2 Uncultured marine eukaryote clone NW414.31 DQ119928 92.6 1051 689/744 2 5 Uncultured alveolate clone RA010613.20 DQ186535 93.3 1079 691/741 5 10 6 Uncultured eukaryote clone SCM37C21 AY664990 93.8 1101 695/741 2 9 Uncultured marine eukaryote clone ENVP36162.00243 DQ918787 94.9 459 279/294 3 12 Uncultured eukaryote clone SCM37C21 AY664990 90.8 987 689/759 5 5 18 Uncultured marine picoplankton clone He000327_39 AJ965089 99.4 647 355/357 1 26 Uncultured marine eukaryote clone BL001221.40 AY426886 90.6 983 681/752 2 3 27 Uncultured marine eukaryote clone UEPACAPp3 AY129041 99.6 1301 710/713 3 32 Uncultured alveolate clone RA010613.126 DQ186534 95.7 1182 709/741 1 42 Amoebophrya sp. ex Karlodinium micrum AF472553 97.4 1227 702/721 1 1 1 43 Amoebophrya sp. ex Karlodinium micrum AF472553 96.5 1123 656/680 1 MB10 27 Uncultured alveolate clone RA010412.70 DQ186533 98.2 1127 646/658 1 30 Amoebophrya sp. ex Ceratium tripos AY208892 99.3 1293 712/717 2 46 Amoebophrya sp. ex Ceratium tripos AY208892 100 1260 682/682 1 3 1 51 Uncultured marine picoplankton AP-picoclone8 DQ386744 92.4 1090 720/779 1 TH10 1 Eukaryote clone OLI11012 AJ402330 95.7 1240 749/783 4 4 Uncultured eukaryote clone BB01_83 AY885027 93.7 798 508/542 8 7 Uncultured marine alveolate group II clone DH147-EKD16 AF290071 95.7 1201 718/750 3 12 Amoebophrya sp. ‘Dinophysis’ AF239260 96.5 1219 714/740 2 19 Amoebophrya sp. AY775284 93 1149 742/798 2 22 Amoebophrya sp. ex Karlodinium micrum AF472553 92.2 1110 734/796 1 25 Uncultured alveolate clone RA000907.2 AY295491 95.6 682 409/428 1 27 Uncultured eukaryote clone Q2A03 EF173006 95.8 1194 715/746 2 46 Uncultured marine picoplankton AP-picoclone8 DQ386744 98.4 1179 660/671 1 49 Uncultured marine alveolate group II clone DH147-EKD6 AF290068 91.5 1070 720/787 1 Alveolates group I MB04 17 Uncultured marine eukaryote clone ENVP21819.00079 DQ918313 98.6 1105 616/625 2 MB07 2 Uncultured marine eukaryote clone BL001221.41 AY426887 98.9 1332 739/747 1 11 10 5 5 2 40 Uncultured eukaryote isolate CAR_E214 AY256282 98.1 1301 736/750 1 TH07 0 Uncultured eukaryote isolate A3_E031 AY046773 89.4 806 579/648 1 4 Uncultured marine alveolate isolate 65 DQ916410 88.1 881 667/757 23 TH10 8 Uncultured eukaryote clone SCM37C30 AY665056 97 1345 781/805 1 45 Uncultured eukaryote clone TAGIRI-10 AB191418 99.4 1297 711/715 1 1 57 Uncultured eukaryote clone SCM38C41 AY664995 99.1 1367 755/762 1 Alveolata; Ciliophora MB01 7 Strombidium sp. AY143564 99.3 1208 665/670 2 3 12 Uncultured marine eukaryote clone CD8.16 DQ647517 97.8 1347 763/780 2 1 1 3 1 3 13 Uncultured marine eukaryote clone CD8.16 DQ647517 99.6 1273 696/699 1 1 18 Strombidium sp. AY143564 98.9 1421 789/798 1 21 Uncultured marine eukaryote clone MD65.05 DQ119933 97.6 1347 771/790 5 1 1 26 Uncultured marine eukaryote clone NW414.24 DQ120009 99.1 1421 785/792 1 2 1 35 Uncultured eukaryote clone ENI40076.00328 AY937624 95.5 1068 640/670 1 36 Uncultured eukaryote clone ENI42482.00244 AY938119 99.8 948 515/516 1 1 37 Uncultured eukaryote clone ENI40076.00328 AY937624 99.1 1201 661/667 1 3 1 Table A1 (continued) OTUs OTU frequency Library Clone Closest relative GenBank % similarity BLAST Query/ MB01 MB04 MB07 MB10 TH01 TH04 TH07 TH10 Accession # score Subject Alveolata; Ciliophora TH01 13 Ciliate sp. NCMS0601 AM412525 94.8 1138 698/736 4 MB04 2 Uncultured eukaryote clone ENI40076.00749 AY937854 99.6 953 520/522 3 7 Uncultured eukaryote clone ENI40076.00328 AY937624 99.1 1201 661/667 1 TH04 2 Uncultured marine eukaryote clone NW414.24 DQ120009 97.1 1210 700/721 1 16 Uncultured marine eukaryote clone MD65.18 DQ119937 97.6 1256 718/736 1 21 Uncultured marine eukaryote clone UEPACAIp5 AY129053 98.9 1290 715/723 7 4 37 Uncultured marine eukaryote clone MD65.18 DQ119937 97.9 1321 748/764 1 39 Uncultured eukaryote clone ENI47296.00059 AY938245 97.2 1098 632/650 8 4 42 Uncultured marine eukaryote clone UEPACAIp5 AY129053 98.4 880 494/502 2 1 2 MB07 25 Uncultured eukaryote clone D3P05C08 EF100279 93.8 1062 675/720 2 30 Strombidium sp. AY143564 95.3 1166 707/742 1 38 Cryptocaryon irritans AF351579 93.6 1129 714/763 1 MB10 16 Strobilidium caudatum AY143573 94.9 1110 682/719 1 31 Tintinnopsis dadayi AY143562 95.5 1240 748/783 2 33 Strobilidium caudatum AY143573 94.4 1092 678/718 1 36 Strombidinopsis sp. AM412524 95.8 688 408/426 2 40 Parastrombidinopsis shimi AJ786648 93.8 989 623/664 1 TH10 18 Uncultured eukaryote clone SCM15C2 AY665093 94.5 1229 761/805 1 20 Uncultured eukaryote clone SCM28C124 AY665085 99.5 1423 780/784 2 24 Uncultured eukaryote clone ENI42482.00186 AY938072 98.6 913 508/515 1 33 Strobilidium caudatum AY143573 93 1075 692/743 7 2 2 34 Strombidium sp. AY143564 99.5 1356 743/747 1 6 1 1 4 1 3 37 Strombidium sp. AY143565 93.4 1146 736/788 1 53 Uncultured marine colpodean ciliate clone DH147-EKD23 AF290076 88.9 941 706/794 1 1 1 58 Eutintinnus pectinis AF399170 93.9 1105 694/739 2 1 Alveolata; Dinophyceae MB01 6 Uncultured eukaryote clone SCM37C24 AY664894 98.7 1395 777/787 8 4 27 Uncultured marine eukaryote clone BL001221.15 AY426867 99.1 1421 785/792 1 2 33 Uncultured eukaryote clone SCM15C23 AY664993 99 1413 783/791 2 1 40 Uncultured eukaryote clone SCM37C27 AY664962 99.4 1432 786/791 1 1 2 TH01 22 Uncultured marine eukaryote clone BL001221.42 AY426888 99.1 1238 682/688 1 MB04 15 Uncultured eukaryote clone SCM28C60 AY664961 95.8 1255 749/782 3 34 Pyrodinium bahamense var. compressum DQ500120 96.1 1199 709/738 1 39 Uncultured eukaryote clone SCM27C15 AY664924 98.5 1297 724/735 1 1 TH07 15 Uncultured marine eukaryote clone CD8.10 DQ647514 100 1295 701/701 2 1 MB10 3 Ceratium longipes DQ388462 99.6 1291 705/708 2 3 1 2 10 Ceratium longipes DQ388462 99.3 981 539/543 1 23 Noctiluca scintillans DQ388461 97.1 1158 667/687 1 29 Pentapharsodinium sp. AF274270 96.7 1240 722/747 3 52 Noctiluca scintillans DQ388461 99.6 1459 797/800 1 5 TH10 6 Uncultured eukaryote clone SCM37C27 AY664962 99.8 1016 552/553 1 10 Uncultured marine picoplankton AP-picoclone12 DQ386748 98.5 1301 729/740 1 Table A1 (continued) OTUs OTU frequency Library Clone Closest relative GenBank % similarity BLAST Query/ MB01 MB04 MB07 MB10 TH01 TH04 TH07 TH10 Accession # score Subject Chlorophyta; Prasinophyceae MB01 5 Micromonas pusilla AY425316 99.5 1430 783/787 4 22 Micromonas pusilla AB183589 99.7 1454 792/794 3 1 2 30 Uncultured prasinophyte clone NW414.32 DQ055165 99.7 1437 782/784 2 1 3 31 Micromonas pusilla AB183589 97.5 1351 775/795 1 38 Micromonas pusilla AB183589 98 1040 589/601 1 TH01 40 Ostreococcus sp.