1707 W. FM 1626 Manchaca, Texas 78652 www.zaraenvironmental.com FINAL REPORT FOR DEEP AQUIFER BIOTA STUDY OF THE EDWARDS AQUIFER Prepared for: Edwards Aquifer Authority 1615 N. St. Mary's Street San Antonio, TX 78215 30 December 2010 TABLE OF CONTENTS Executive Summary ................................................................................................ 4 Introduction ........................................................................................................... 5 Project Logistics ...................................................................................................... 5 Project Development ............................................................................................... 5 Sample Location Criteria ....................................................................................... 6 Edwards Aquifer Fauna Sampling .............................................................................. 7 Methods ................................................................................................................. 8 Locating Sample Sites ............................................................................................. 8 Establishing Landowner Agreements (Well Letters) .................................................. 8 Biological Sampling Procedures ............................................................................... 11 Trapping Methods .............................................................................................. 11 Specimen Collection and Identification ..................................................................... 14 Results ................................................................................................................. 15 Historic Blind Catfish sites ...................................................................................... 21 Artesia Pump Station Well .................................................................................. 21 Artesian well in San Antonio................................................................................ 23 Bexar Metropolitan Water District Well ................................................................. 23 El Patio Foods Well ............................................................................................ 24 El Patio Foods Well no. 2 .................................................................................... 26 George W. Brackenridge Well .............................................................................. 26 Josef Boecke Well .............................................................................................. 27 O.R. Mitchell Well .............................................................................................. 27 Verstraeten Well No. 1 ....................................................................................... 28 William Kempin Well .......................................................................................... 29 Collaborative Results ............................................................................................. 31 Fish Collections ................................................................................................. 31 Crustacean Collections ....................................................................................... 32 Microbial analysis .............................................................................................. 33 Stable isotope analysis ....................................................................................... 33 Current Study Results ........................................................................................... 34 New locality records ........................................................................................... 34 Aldridge 209 Well .............................................................................................. 35 Aldridge Corporate Well ...................................................................................... 42 Aquarena Springs Well ....................................................................................... 45 Bexar Met Well #8 ............................................................................................. 45 Bexar Met Well #91 ........................................................................................... 45 Carroll Farms Well ............................................................................................. 46 City of Kyle Well ................................................................................................ 46 Constanzo Well #2 ............................................................................................ 47 Fort Sam Houston Well #2 .................................................................................. 48 Fort Sam Houston Well #5 .................................................................................. 50 Girl Scout Well .................................................................................................. 51 Homer Verstuyft Well ......................................................................................... 52 Hondo Index Well .............................................................................................. 55 Jeff Bailey Well .................................................................................................. 55 LCRA Well ......................................................................................................... 56 Loop 353 Well ................................................................................................... 57 2 Lyda (Fallon) Partin Well .................................................................................... 60 Mark Verstuyft Well ........................................................................................... 60 Mission Bowling Well .......................................................................................... 60 Nelson Road Well ............................................................................................... 61 New Braunfels Transect Wells ............................................................................. 62 Panther Canyon Well .......................................................................................... 62 Paradise Alley Well ............................................................................................ 64 Persyn Well ....................................................................................................... 64 Raymond Wauters Well ...................................................................................... 65 Roger Verstuyft Well .......................................................................................... 66 Roosevelt Bridge Springs .................................................................................... 67 San Antonio Zoo Well ......................................................................................... 68 San Marcos Flagpole Well ................................................................................... 70 Schumann Artesian Well ..................................................................................... 71 Schumann House Well ........................................................................................ 71 Shavano Park #5 ............................................................................................... 73 Steve’s Homestead (San Antonio Conservation Society) ......................................... 73 Stull Farms Well ................................................................................................ 74 Tschirhart Well .................................................................................................. 75 Uvalde Index Well.............................................................................................. 82 Verstuyft Farms Well .......................................................................................... 82 Verstuyft Home Farm Well .................................................................................. 83 Von Ormy Growers Well ..................................................................................... 84 W.H. Guenther Well ........................................................................................... 85 Woodley Farms Well No. 1 .................................................................................. 85 Woodley Farms Well No. 2 .................................................................................. 86 Yanta Well ........................................................................................................ 86 Discussion and Future Work ................................................................................. 88 Staff and Contributors .......................................................................................... 91 Zara Staff ............................................................................................................ 91 Taxonomic Experts ................................................................................................ 92 Bibliography ......................................................................................................... 94 Appendix .............................................................................................................. 97 Glossary ............................................................................................................. 108 3 EXECUTIVE SUMMARY