MAP2K7 Assay platform : Mobility Shift Assay Product code 07-148 Substrate : JNK2 Full-length human MAP2K7 [1-419(end) amino acids of accession Cascade Assay* number NP_660186.1] was co-expressed as N-terminal GST-fusion Metal : Mg protein (75 kDa) with human His-tagged MAP3K3 [1-626(end) amino acids of accession number NP_002392.2] using baculovirus Reference compound : Staurosporine expression system. GST-MAP2K7 was purified by using glutathione IC50 at 1 mM ATP (nM) : 1100 sepharose chromatography. *JNK2/Modified Erktide MAP3K1 Assay platform : Mobility Shift Assay Product code 07-103 Substrate : MAP2K1 Human MAP3K1, catalytic domain [1327-1646(end) amino acids of Cascade Assay* accession number XP_042066.8] was expressed as N-terminal Metal : Mg GST-fusion protein (62 kDa) using baculovirus expression system. GST-MAP3K1 was purified by using glutathione sepharose Reference compound : Staurosporine chromatography and anion exchange chromatography. IC50 at 1 mM ATP (nM) : 160 *MAP2K1/Erk2/Modified Erktide MAP3K2 Assay platform : Mobility Shift Assay Product code 07-104 Substrate : MAP2K4/MAP2K7 Human MAP3K2, catalytic domain [337-620(end) amino acids of Cascade Assay* accession number NP_006600.3] was expressed as N-terminal Metal : Mg GST-fusion protein (59 kDa) using baculovirus expression system. GST-MAP3K2 was purified by using glutathione sepharose Reference compound : Staurosporine chromatography. IC50 at 1 mM ATP (nM) : 45 *(MAP2K4/MAP2K7)/JNK2/Modified Erktide MAP3K3 Assay platform : Mobility Shift Assay Product code 07-105 Substrate : MAP2K6 Full-length human MAP3K3 [1-626(end) amino acids of accession Cascade Assay* number NP_002392.2] was expressed as N-terminal GST-fusion Metal : Mg protein (98 kDa) using baculovirus expression system. GST-fusion MAP3K3 was purified by using glutathione sepharose Reference compound : Staurosporine chromatography. IC50 at 1 mM ATP (nM) : 72 *MAP2K6/p38 α/Modified Erktide MAP3K4 Assay platform : Mobility Shift Assay Product code 07-106 Substrate : MAP2K6 Human MAP3K4, catalytic domain [1312-1608(end) amino acids of Cascade Assay* accession number NP_005913.2] was expressed as N-terminal Metal : Mg GST-fusion protein (61 kDa) using baculovirus expression system. GST-MAP3K4 was purified by using glutathione sepharose Reference compound : Staurosporine chromatography. IC50 at 1 mM ATP (nM) : 100 *MAP2K6/p38 α/Modified Erktide 71.