Leader in Biomolecular Solutions for Life Science Fascin/FSCN1 Rabbit mAb Catalog No.: A9566 Recombinant Basic Information Background Catalog No. This gene encodes a member of the fascin family of actin-binding proteins. Fascin A9566 proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell Observed MW migration, motility, adhesion and cellular interactions. Expression of this gene is known 54KDa to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this Calculated MW gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of 54kDa this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011] Category Primary antibody Applications WB, IHC Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 6624 Q16658 IHC 1:50 - 1:200 Immunogen A synthesized peptide derived from human Fascin/FSCN1/FSCN1 Synonyms FAN1; HSN; SNL; p55 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using Fascin/FSCN1/FSCN1 Rabbit mAb (A9566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- embedded rat spleen using embedded human esophageal cancer embedded mouse spleen using Fascin/FSCN1/FSCN1 Rabbit mAb (A9566) using Fascin/FSCN1/FSCN1 Rabbit mAb Fascin/FSCN1/FSCN1 Rabbit mAb (A9566) at dilution of 1:100 (40x lens). (A9566) at dilution of 1:100 (40x lens). at dilution of 1:100 (40x lens). Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..