European Journal of Immunology Supporting Information for Eur. J. Immunol. 10.1002/eji.200535496 Alternative activation deprives macrophages of a coordinated defense program to Mycobacterium tuberculosis Antje Kahnert, Peter Seiler, Maik Stein, Silke Bandermann, Karin Hahnke, Hans Mollenkopf and Stefan H. E. Kaufmann © 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.eji.de ΣΣΣ ↑↓ 163 ↑ 143 > 0 anticorrelated ↓ 20 I III II ↑↓ 105 ↑↓ 39 ↑↓ 19 ↑ 86 ↓ 19 ↑ 38 ↓ 1 ↑ 19 ↓ 0 IFN- γγγ 4h IL-4 4h ↑↓ 144 ↑↓ 58 ↑ 124 ↓ 20 ↑ 57 ↓ 1 ΣΣΣ ↑↓ 1331 ↑ 680 > 41 anticorrelated ↓ 692 IV VI V ↑↓ 943 ↑↓ 159 ↑↓ 229 ↑ 411 ↓ 573 ↑ 72 ↓ 46 ↑ 197 ↓ 73 IFN- γγγ 48h IL-4 48h ↑↓ 1102 ↑↓ 388 ↑ 483 ↓ 619 ↑ 269 ↓ 119 IFN- γγγ 4h IL-4 4h Group Fold Change P-value Intensity1 Intensity2 Fold Change P-value Intensity1 Intensity2 Accession # Primary Sequence Name Sequence Description I ↑↑↑ 4.37371 3.18166E-32 657.5683 2877.62183 1.52007 0.22311 124.05638 189.28168 NM_008042 Fprl1 formyl peptide receptor-like 1 I ↑↑↑ 5.19517 2.30606E-23 3173.28125 16666.12305 1.24572 0.04476 1087.70605 1344.94006 NM_011315 Saa3 serum amyloid A 3 I ↑↑↑ 2.05258 3.2031E-06 1358.04822 2813.76611 1.10341 0.60242 697.22046 783.63538 NM_008518 Ltb lymphotoxin B I ↑↑↑ 2.62754 1.24797E-11 3477.42529 9092.96484 1.10201 0.29205 910.6438 1004.36945 NM_008357 Il15 interleukin 15 I ↑↑↑ 2.19028 5.38095E-09 1189.62219 2618.71338 1.83042 0.00007 367.65424 674.255 NM_007548 Prdm1 PR domain containing 1, with ZNF domain I ↑↑↑ 3.16446 4.00706E-07 520.12781 1650.31921 2.23118 0.10955 76.51717 166.07359 NM_009421 Traf1 Tnf receptor-associated factor 1 I ↑↑↑ 2.11787 0.00005 4171.9834 8799.2832 1.47311 0.02326 3451.87598 5111.30762 NM_008152 Gpr65 G-protein coupled receptor 25 I ↑↑↑ 2.91776 4.26711E-10 11832.90039 34579.65234 1.99595 1 9310.50977 18565.26172 NM_009058 Ralgds ral guanine nucleotide dissociation stimulator I ↑↑↑ 4.09402 2.53194E-08 161.28786 660.95789 2.11944 0.12092 81.33522 161.59579 NM_010510 Ifnb1 interferon beta, fibroblast I ↑↑↑ 2.05494 1.21879E-08 632.34619 1300.89612 1.32752 0.03384 599.34027 797.30957 NM_013839 Nr1h3 nuclear receptor subfamily 1, group H, member 3 I ↑↑↑ 2.71144 2.34192E-20 1090.38696 2955.12793 1.80198 1.23369E-06 479.62128 867.71027 NM_021327 Tnip1 Nef-associated factor 1 I ↑↑↑ 6.6489 3.14482E-35 9534.8623 63295.4375 1.71771 0.00016 3515.78125 6029.41797 NM_011611 Tnfrsf5 tumor necrosis factor receptor superfamily, member 5 I ↑↑↑ 2.00794 4.42169E-10 3434.86133 6906.89746 1.74663 0.0003 7131.7998 12386.69336 AW496438 AW496438 ESTs, Moderately similar to KIAA0769 protein [H.sapiens] I ↑↑↑ 8.21634 5.53599E-11 126.95619 1063.02893 2.06777 0.17241 80.90722 142.85664 M86671 Il12b interleukin 12b I ↑↑↑ 3.08212 2.66256E-17 2299.72461 7106.50586 1.44944 0.00882 423.29254 612.73071 AI043056 X83327 EST X83327 I ↑↑↑ 2.04268 2.18937E-07 2181.60986 4447.53076 1.16099 0.06864 555.50269 644.98157 AI450152 AI450152 ESTs I ↑↑↑ 2.21743 2.88452E-18 3962.47314 8788.41602 1.46842 0.00016 2246.23047 3309.95142 AI326880 AI326880 ESTs, Highly similar to ZINC FINGER PROTEIN 7 [Homo sapiens] I ↑↑↑ 2.67948 3.68232E-06 164.38525 439.54199 2.1368 0.0086 63.8369 137.24866 NM_009397 Tnfaip3 tumor necrosis factor, alpha-induced protein 3 I ↑↑↑ 2.00609 1.57233E-08 191.7652 383.59735 1.85161 4.41058E-08 341.56604 631.33923 AF068748 Sphk1 sphingosine kinase 1 I ↑↑↑ 9.23315 3.43792E-34 4778.18652 44401.78906 -1.00508 0.98534 389.77069 398.48138 NM_019494 Cxcl11 small inducible cytokine subfamily B (Cys-X-Cys), member 11 I ↑↑↑ 2.11276 3.56719E-09 24075.94531 50742.71094 2.13895 0.00096 12943.9873 27685.65234 NM_013562 Ifrd1 interferon-related developmental regulator 1 I ↑↑↑ 2.83923 1.5042E-08 433.24518 1246.83594 1.70746 0.0015 220.08766 383.80035 BB532105 BB532105 ESTs I ↑↑↑ 2.64545 0.00037 18511.69336 49544.16797 1.66114 0.04491 11596.39258 19641.90039 NM_008102 Gch1 GTP cyclohydrolase 1 I ↑↑↑ 5.02574 0 177.73532 893.58356 1.8875 0.06782 77.14024 146.35068 NM_008361 Il1b interleukin 1 beta I ↑↑↑ 3.69403 4.6101E-39 983.32678 3628.97119 1.45525 0.09065 78.51878 115.46391 BE197977 BE197977 ESTs, Moderately similar to GPRI_HUMAN PROBABLE G PROTEIN-COUPLED RECEPTOR GPR18 [H.sapiens] I ↑↑↑ 2.88868 3.47018E-07 683.92633 2005.10156 1.77378 0.01264 1403.203 2505.11768 M12571 Hspa1a heat shock protein, 70 kDa 3 I ↑↑↑ 2.86649 3.6846E-34 293.7431 841.94922 1.58011 0.08123 70.09181 112.63826 AI481176 AI481176 ESTs I ↑↑↑ 2.00309 3.90637E-06 3355.87695 6695.66553 1.48713 0.0008 1102.73145 1636.25061 BF018243 BF018243 ESTs, Weakly similar to neuronal tyrosine threonine phosphatase 1 [M.musculus] I ↑↑↑ 3.40016 2.79279E-42 1994.36853 6780.56152 1.81468 8.6822E-10 544.91968 990.34894 K02782 C3 complement component 3 I ↑↑↑ 2.89001 7.9468E-08 188.33945 544.81531 1.66649 0.00778 61.42165 102.44063 NM_013521 Fpr1 formyl peptide receptor 1 I ↑↑↑ 2.29034 0.00003 5634.97363 12982.96094 1.20636 0.29849 3055.42603 3737.53076 BE630688 BE630688 Mus musculus SAMSN1 mRNA, complete cds I ↑↑↑ 2.07181 0.00022 1336.53442 2771.52563 -1.062 0.76551 1377.33862 1301.38 NM_009366 Tgfb1i4 transforming growth factor beta 1 induced transcript 4 I ↑↑↑ 2.66471 0.00097 19612.94531 53320.54688 1.51894 0.22443 11305.49121 17279.91406 NM_011018 Sqstm1 oxidative stress induced I ↑↑↑ 2.35207 6.2387E-09 6482.23486 15209.37109 1.33676 0.06506 17149.98438 22561.96484 AF077742 Tcfec transcription factor EC I ↑↑↑ 2.27641 0.00026 1901.43042 4374.19873 1.44067 0.0686 1278.10059 1895.49438 BB206914 BB206914 EST, Moderately similar to TDA8_MOUSE PROBABLE G PROTEIN-COUPLED RECEPTOR TDAG8 [M.musculus] I ↑↑↑ 2.29113 1.62838E-22 762.08685 1746.00146 1.4563 0.00021 405.23706 593.46002 AF199491 Ccrn4l Mus musculus NOCTURNIN (Nocturnin) mRNA, complete cds I ↑↑↑ 4.05415 3.18035E-22 5545.58887 22339.75977 1.7557 5.13114E-08 2804.34302 4899.48242 NM_009841 Cd14 CD14 antigen I ↑↑↑ 3.58344 4.8033E-29 1387.31335 4966.97461 1.38684 0.02403 108.1729 149.59058 NM_008039 Fpr-rs2 formyl peptide receptor, related sequence 2 I ↑↑↑ 2.23795 0.00008 3286.36963 7412.73828 1.44845 0.04014 2613.5498 3863.97217 BB201573 BB201573 EST, Moderately similar to TDA8_MOUSE PROBABLE G PROTEIN-COUPLED RECEPTOR TDAG8 [M.musculus] I ↑↑↑ 2.87144 4.6969E-14 596.01801 1711.35645 1.03944 0.82593 299.34717 315.59778 NM_008903 Ppap2a phosphatidic acid phosphatase 2a I ↑↑↑ 3.64964 0 2853.29468 10410.93164 1.52968 4.5241E-06 1810.38037 2764.46729 BF020125 BF020125 ESTs, Moderately similar to unnamed protein product [H.sapiens] I ↑↑↑ 4.17109 1.47823E-41 1067.51404 4457.05078 1.67225 6.66339E-09 581.08875 970.03125 AI256591 AI256591 ESTs I ↑↑↑ 2.21896 7.01942E-14 1157.37402 2569.28491 1.65634 5.40206E-06 1713.38989 2851.83643 AI385691 AI385691 ESTs, Highly similar to P2UR_MOUSE P2U PURINOCEPTOR 1 [M.musculus] I ↑↑↑ 3.96552 0 1402.82056 5562.91992 1.12731 0.48849 456.55466 523.0871 NM_011171 Procr protein C receptor, endothelial I ↑↑↑ 2.71738 1.0795E-08 187.99223 503.92175 1.90544 0.00006 126.05093 240.19389 NM_009705 Arg2 arginase type II I ↑↑↑ 3.78329 5.84249E-07 38.94953 149.17516 1.03208 0.89196 33.66799 46.68737 NM_008335 Ifna6 interferon alpha family, gene 8 I ↑↑↑ 3.0662 1.57409E-34 1143.69238 3507.18066 1.18259 0.49032 200.3089 243.61404 NM_007981 Acsl1 fatty acid Coenzyme A ligase, long chain 2 I ↑↑↑ 2.21769 0.00007 744.73987 1634.55273 1.5401 0.05251 387.71252 592.02271 U70139 Ccrn4l carbon catabolite repression 4 homolog (S. cerevisiae) I ↑↑↑ 2.29641 4.18685E-13 108.651 249.66084 1.21863 0.39744 54.1762 66.04936 NM_008334 Ifna7 interferon alpha family, gene 7 I ↑↑↑ 2.06633 9.72754E-10 35713.98438 73443.47656 -1.09533 0.3712 5063.5083 4606.9209 BE989134 BE989134 ESTs I ↑↑↑ 2.11446 4.86847E-10 1026.84802 2162.50024 1.1586 0.49888 161.42722 186.97969 AI662827 AI662827 EST I ↑↑↑ 8.7791 0 134.91577 1185.65088 -1.46506 0.51574 56.97549 48.10512 NM_010927 Nos2 nitric oxide synthase 2, inducible, macrophage I ↑↑↑ 2.11885 1.86993E-18 515.1369 1090.88196 -1.78033 2.89755E-08 1350.4718 761.11597 NM_008059 G0s2 G0/G1 switch gene 2 I ↑↑↑ 2.40796 1.38279E-20 1853.20093 4464.31006 1.42495 0.0017 550.86298 783.47217 AW494132 AW494132 ESTs I ↑↑↑ 2.77651 7.98765E-19 2321.85669 6405.04785 1.26154 0.31297 7285.89502 9191.80273 NM_008808 Pdgfa platelet derived growth factor, alpha I ↑↑↑ 2.2269 0.0015 2952.04736 6459.98193 1.28441 0.24627 1200.33838 1521.604 NM_016767 Batf basic leucine zipper transcription factor, ATF-like I ↑↑↑ 2.0958 9.06144E-18 4420.35547 9268.5957 1.38628 0.00017 2662.11426 3686.81592 AI644220 AI644220 ESTs, Moderately similar to AF151825_1 CGI-67 protein [H.sapiens] I ↑↑↑ 2.86253 1.7611E-31 380.98676 1091.64966 1.38586 0.02997 288.3887 403.92831 AI450672 AI450672 ESTs I ↑↑↑ 2.4308 5.379E-14 325.24634 792.36566 1.07865 0.5865 130.77739 141.36136 BE634799 BE634799 ESTs I ↑↑↑ 2.59239 2.69375E-23 232.59319 603.50049 1.05781 0.76035 101.11175 107.39954 NM_019564 Prss11 insulin-like growth factor binding protein 5 protease I ↑↑↑ 3.52777 1.84335E-15 121.72289 435.10095 1.3962 0.20785 104.27471 140.54333 NM_010554 Il1a interleukin 1 alpha I ↑↑↑ 2.22767 5.81309E-13 204.85254 454.69531 1.18062 0.114 404.96429 476.79102 NM_007413 Adora2b adenosine A2b receptor I ↑↑↑ 2.24517 3.98988E-12 2791.5459 6245.95215 1.80281 8.02644E-06 791.17444 1417.79346 NM_019777 Ikbke inducible IKappaB kinase I ↑↑↑ 2.42382 7.25447E-08 715.04272 1743.58276 1.15808 0.4743 302.71609 359.75955 NM_010612 Kdr kinase insert domain protein receptor I ↑↑↑ 2.16073 7.65005E-14 45766.27344 99207.39062 1.50038 0.00006 39259.45312 59076.94141 NM_013885 Clic4 chloride intracellular channel 4 (mitochondrial) I ↑↑↑ 2.50669 8.07447E-09