US010328026B2 (12 ) United States Patent ( 10 ) Patent No. : US 10 , 328 , 026 B2 Thaxton et al. (45 ) Date of Patent: * Jun . 25 , 2019 ( 54 ) SYNTHETIC NANOSTRUCTURES (58 ) Field of Classification Search INCLUDING NUCLEIC ACIDS AND /OR CPC .. .. .. .. A61K 9 / 127 OTHER ENTITIES See application file for complete search history . (71 ) Applicant: Northwestern University, Evanston , IL (56 ) References Cited (US ) U . S . PATENT DOCUMENTS (72 ) Inventors : C . Shad Thaxton , Chicago , IL (US ) ; 5 ,472 , 881 A 12 / 1995 Beebe et al. Chad A . Mirkin , Wilmette , IL ( US) ; 5 , 814 ,666 A 9 / 1998 Green et al . Kaylin M . McMahon , Chicago , IL 5 , 955 , 589 A 9 / 1999 Cook et al. (US ) ; Sushant Tripathy , Evanston , IL 6 , 274 , 337 B1 8 / 2001 Parce et al. (US ) ; Raja Kannan Mutharasan , 6 , 361, 944 B1 3 / 2002 Mirkin et al. 6 ,506 , 564 B1 1 / 2003 Mirkin et al. Chicago , IL ( US ) ; David M . 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( Continued ) 61/ 296 , 373 , filed on Jan . 19 , 2010 . Primary Examiner — Scott Long ( 74 ) Attorney , Agent, or Firm — Wolf, Greenfield & (51 ) Int. Cl. Sacks, P . C . A61K 9 / 127 ( 2006 .01 ) A61K 31/ 7088 ( 2006 .01 ) ( 57 ) ABSTRACT B82Y 5700 ( 2011 . 01) Articles , compositions, kits , and methods relating to nano C12N 15 / 87 ( 2006 . 01) structures , including synthetic nanostructures, are provided . A61K 9 /51 ( 2006 . 01 ) Certain embodiments described herein include structures A61K 47 /54 ( 2017 . 01 ) having a core -shell type arrangement ; for instance , a nano A61K 47 /69 ( 2017 .01 ) structure core may be surrounded by a shell including a C12N 15 / 11 ( 2006 .01 ) material , such as a lipid bilayer , and may include other ( 52 ) U . S . CI. components such as oligonucleotides. In some embodi CPC . .. .. .. A61K 9 / 127 ( 2013 . 01 ) ; A61K 9 / 1271 ments , the structures, when introduced into a subject , can be ( 2013 .01 ) ; A61K 9 /5115 ( 2013 .01 ) ; A61K used to deliver nucleic acids and / or can regulate gene 9 /5123 (2013 .01 ) ; A61K 31/ 7088 ( 2013 .01 ) ; expression . Accordingly , the structures described herein A61K 47 /544 ( 2017 .08 ) ; A61K 47 /554 may be used to diagnose , prevent, treat or manage certain ( 2017 .08 ) ; A61K 47 /6917 ( 2017 . 08 ) ; A61K diseases or bodily conditions. In some cases, the structures 4776923 (2017 .08 ) ; B82Y 5 / 00 ( 2013 .01 ) ; are both a therapeutic agent and a diagnostic agent. C12N 15 / 87 ( 2013 .01 ) ; CI2N 15 / 111 ( 2013 . 01 ) ; C12N 2310 / 113 ( 2013 .01 ) ; C12N 20 Claims, 21 Drawing Sheets 2310 / 3515 ( 2013 .01 ) ; CI2N 2320 / 32 ( 2013 .01 ) Specification includes a Sequence Listing . US 10 ,328 ,026 B2 Page 2 References Cited WO WO 2010 /017152 2 /2010 ( 56 ) WO WO 2010 /017154 2 /2010 U . S . 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