Vol. 104 (1) The Ohio Journal of Science A-5. Index to Sessions in DeBartolo Hall Ohio Branch American Society for Microbiology Poster Session-Multidisciplines 09:00 AM – 10:00 AM p. 6 The Ohio Branch Poster Session-Multidisciplines of The American Society for Microbiology 10:00 AM – 11:00 AM p. 13 (OBASM) will meet jointly with The Ohio Academy of Science. Poster Session-Multidisciplines Please see the following schedule. or contact: 02:00 PM – 03:00 PM p. 20 Dr. Chet Cooper at Youngstown State University. Phone 330-941-1361; fax 330-941-1483 or by email [email protected] Pre-College Poster Session 03:00 PM – 04:30 PM p. 27 Saturday, April 17th 8:00 AM – 3:00 PM Registration Physical Sciences & Education p. 31 8:30 AM Welcome - Auditorium, DeBartolo Hall 09:00 AM Saturday, April 17, 2004 DeBartolo Hall Room 347 9:00 AM – 10:00 AM Dr. Paul Szalay-Presiding My virus is nastier than your virus!! Strain dependent differences in the molecular properties of the ICP34.5 protein of HSV-1 that determine the virulence of HSV-1 Zoology p. 32 Dr. Ken Rosenthal, Northeastern Ohio 09:00 AM Saturday, April 17, 2004 Universities College of Medicine DeBartolo Hall Room 358 Dr. Courtenay Willis-Presiding 10:00 AM - 11:00 AM General Microbiology Lecture Phycology & Aquatic Ecology p. 33 Cepacia means more than onions 09:00 AM Saturday, April 17, 2004 Dr. Christine Weingart DeBartolo Hall Room 356 Denison University Dr. Robert Heath-Presiding 11:15 AM – 12:15 AM Roundtable: Intellectual Property Basic & Applied Microbiology p. 35 1:30 PM – 3:00 PM Molecular Biology Forum: 50 Years 09:00 AM Saturday, April 17, 2004 of the Double Helix DeBartolo Hall Room 346 Co-sponsored by Sigma Xi, YSU Chapter Dr. Paul Baker-Presiding Chaired by Dr. Diana Fagan, Department of Biological Sciences, Youngstown State University Earth & Environmental Sciences p. 36 09:30 AM Saturday, April 17, 2004 Clinical Applications of Genomics and Proteomics DeBartolo Hall Room 345 Dr. David Buzzee, Lab Corporation of Ohio Mr. Wilmer Stowe-Presiding Ancient DNA Dr. Bruce Rothschild, Northeastern Ohio Universities Genetics, Biochemistry & Physiology p. 38 College of Medicine 02:00 PM Saturday, April 17, 2004 Other participants to be announced DeBartolo Hall Room 358 Dr. Kerry Cheesman-Presiding 3:00 PM – 5:00 PM “Late Breaker” Session – brief oral presentations on recent discoveries in the microbiological Plant Ecology/Wetlands p. 39 sciences 02:00 PM Saturday, April 17, 2004 5:00 PM OBASM General Meeting DeBartolo Hall Room 356 Mr. Tracy Engle-Presiding 6:00 PM -OBASM Dinner and Student Awards Chestnut Room, Kilcawley Center. Contact Dr. Chet Cooper by April 9th. $16.00 A-6. The Ohio Journal of Science Vol. 104 (1) with wheat, the expected nitrate should be present at high levels. 9:00 am Poster Session De Bartolo Hall Ten samples of soil were taken from the forest and adjacent farmland at 1-meter intervals on three different dates. The soil samples BOARD 01 THE DEVELOPMENT OF A were extracted in a potassium chloride (1M) solution. Extracts will GEOLOGICAL SEQUESTRATION SIMULATION be analyzed spectrophotometrically with a Flow Injection Analyzer FACILITY (GSSF) TO ADVANCE THE UNDERGROUND and values will be reported on a per gram of dry weight and organic STORAGE OF ANTHROPOGENIC CARBON DIOXIDE. matter basis. KENNETH A. LASOTA ([email protected]) DEPT OF N ATURAL SCIENCES, ROBERT MORRIS UNIVERSITY, 600 FIFTH AVE, BOARD 04 EVALUATION OF AN IN VITRO ASSAY PITTSBURGH PA 15219-3099. FOR PREDICTING FUNGICIDE EFFICACY AGAINST SCLEROTINIA HOMOEOCARPA IN THE FIELD. AMY L. The production worldwide of more than nine billion tons of carbon MIKUSZEWSKI, [email protected], YOUNG K. JO, dioxide each year by industrial processes is affecting greenhouse [email protected], JOSEPH W. RIMELSPACH, [email protected] gas concentrations in the atmosphere. The injection and AND MICHAEL J, BOEHM, [email protected], DEPT OF PLANT sequestration of carbon dioxide scrubbed from the flue gases of PATHOLOGY, THE OHIO S TATE UNIVERSITY, 201 KOTTMAN HALL, coal-fired power plants into brine aquifers is an option to mitigate 2021 COFFEY RD, COLUMBUS OH 43210-1087. this problem being investigated by the US Department of Energy’s National Energy Technology Laboratory (NETL) in Bruceton, Dollar spot, caused by the fungus Sclerotinia homoeocarpa F. T. Pennsylvania. However, as the behavior of carbon dioxide in Bennett, is one of the most commonly occurring turfgrass diseases underground brine aquifers is not well understood, NETL is in temperate and subtropical regions and the most widespread and developing a Geological Sequestration Simulation Facility (GSSF) chronic disease of golf course turf in Ohio. The disease is typically to gather data on the behavior of carbon dioxide, brine and reservoir managed through the combined use of a balanced fertility program, rock in conditions that exist at depth. The GSSF is envisioned as cultural practices aimed at reducing extended periods of leaf being capable of subjecting carbon dioxide, brine and a variety of wetness and timely applications of fungicides. Resistance in reservoir rocks to the pressure and temperature conditions that Sclerotinia homoeocarpa to various classes of fungicides has been exist at depths up to 3,000 meters. The GSSF is envisioned as reported. The goal of this work was to determine the ability of a being instrumented to record real time as well a pre- and post- test previously described in vitro fungicide screening assay to predict data. Data acquisition is envisioned as being generated by fungicide efficacy in the field. Sclerotinia homoeocarpa isolates were instruments such as MRI, CAT scan, temperature and pressure recovered from 42 golf course fairways throughout Ohio and sensors, x-ray diffraction, atomic absorption, and petrographic screened using the in vitro assay to determine their relative imaging equipment, among others. This study reports on the initial sensitivities to thiophanate-methyl, propiconazole and iprodione. progress in the development of the GSSF, including estimated cost, Each isolate was placed onto potato dextrose agar (PDA) and a proposed instrumentation specifications and timeline for series of fungicide-amended PDA plates. Fungicide sensitivities development. for each isolate were calculated based on relative growth rates of the isolates on PDA versus fungicide-amended PDA. Based on fungicide sensitivity profiles of the 42 isolates, replicated field BOARD 02 CHARACTERIZATION OF HYDRIC SOILS IN WETLAND MITIGATION SITES IN fungicide efficacy trials were established on fairways at 12 Central Ohio golf courses. Fungicide applications were made and dollar CENTRAL OHIO. KIMBERLY A. PREEST, spot severity was rated on a biweekly basis from April to July [email protected], (JAMES L. DOOLEY, 2003. The in vitro screening assay was an excellent predictor of [email protected]), DEPT OF BIOLOGY, MUSKINGUM COLLEGE, thiophanate-methyl, iprodione and propiconazole efficacy in the 163 STORMONT ST, NEW CONCORD OH 43762. field except for isolates with propiconazole EC50 values ranging between 0.03 and 0.04. This ongoing study will examine the hydric soil characteristics of three central Ohio wetland areas. The first of the three study sites is a natural wetland site located in Pickerington Ponds Metro Park BOARD 05 EFFECTS OF ROUNDUP™ ON in Fairfield County, a 1200-acre park in the Blacklick Creek DEVELOPING RANA PIPIENS TERA M. ROBINSON Watershed. The second site is a mitigated wetland located in ([email protected]), (DAVID L. REED) DEPT OF NATURAL Three Creeks Metro Park also in Franklin County, a 74.1-acre site SCIENCES, THE DEFIANCE COLLEGE, 1774 WHITEHALL DR. LIMA, that is fed by Blacklick Creek. The third and final site of this study OH 45805. is the New Albany Wetland Nature Preserve in Delaware County; a 30-acre mitigated wetland site in the Rocky Fork Watershed Over the last fifty years, many species of amphibians in the Untied established by the Ohio Department of Transportation (ODOT). Starts are experiencing a major decline in their numbers. United The Rocky Fork Site is currently considered to be successful by States, agriculture is a major employment factor and with agriculture the Environmental Protection Agency (EPA). Data for this study there is runoff and over use of pesticides and fertilizers. Pesticides will consist of six random core samples from all three wetland and fertilizers in the water systems are factors in the abnormalities sites. These samples will be tested for the presence iron or found in frogs. Pesticides and fertilizers are harming the frogs by manganese concretions, and organic matter (percent of organic adversely affecting their immune system and causing them to be matter will be tested via loss-on-ignition (LOI) technique). Samples more susceptible to parasites and other infections. These will be analyzed to determine whether the soil is a mineral soil, the chemicals are also responsible for the decreased rate of growth, presence of sulfidic material, as well as their aquic/peraquic for example frogs are being found with missing or retained limbs. moisture regime. The soil will be observed for evidence of reducing In this study, Rana pipiens tadpoles in one ten gallon tank will be conditions, and proper matrix chromas, and mottling. exposed to a constant 3.4 grams of Roundup and the other ten gallon tank will receive an increasing amount of 0.1 gram a day for BOARD 03 TEMPORAL AND SPATIAL PATTERNS OF NITRATE AND PHOSPHATE IN AGRICULTURE four months. The development rate will be measured between the AND FOREST SOILS IN SOUTHEASTERN STARK tadpoles by obtaining a mean of the width and length of the tadpoles in different tanks. Also the tadpoles will be monitored daily for COUNTY. FRANK MOROCCO, [email protected], (DR. CHARLES developmental deformities and changes in behavior. The tank that MCCLAUGHERTY [email protected]), MOUNT UNION received a constant 3.4 grams of Roundup had visual abnormality, COLLEGE, 1972 CLARK AVE, ALLIANCE OH 44601.