SAN TA C RUZ BI OTEC HNOL OG Y, INC . MTMR9 (N-15): sc-48244 BACKGROUND SOURCE Myotubularin and the myotubularin-related proteins belong to a highly con - MTMR9 (N-15) is an affinity purified goat polyclonal antibody raised against a served family of eukaryotic phosphatases that utilize inositol phospholipids, peptide mapping near the N-terminus of MTMR9 of human origin. rather than phosphoproteins, as substrates. MTMR9 (myotubularin-related protein 9), also known as C8orf9, is a 549 amino acid protein that belongs to PRODUCT the protein-tyrosine phosphatase family and non-receptor class myotubularin Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide subfamily. Localizing to the cytoplasm, MTMR9 is expressed in many tis - and 0.1% gelatin. sues, including brain. MTMR9 interacts with MTMR6, MTMR7 and MTMR8. As opposed to other members of the myotubularin-related protein family, Blocking peptide available for competition studies, sc-48244 P, (100 µg MTMR9 does not contain a dual-specificity phosphatase domain, and is a peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). probable pseudophosphatase. Containing a double-helical motif similar to the SET interaction domain, MTMR9 may function in the control of cell APPLICATIONS pro liferation. MTMR9 (N-15) is recommended for detection of MTMR9 of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range REFERENCES 1:100-1:1000), immunofluorescence (starting dilution 1:50, dilution range 1. Laporte, J., et al. 1998. Mutations in the MTM1 gene implicated in X- linked 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range myotubular myopathy. ENMC international consortium on myotubular 1:30- 1:3000). myopathy. European neuro-muscular center. Hum. Mol. Genet. 6: 1505- 1511. MTMR9 (N-15) is also recommended for detection of MTMR9 in 2. Blondeau, F., et al. 2000. Myotubularin, a phosphatase deficient in addition al species, including canine, bovine, porcine and avian. myotubular myopathy, acts on phosphatidylinositol 3-kinase and phos - Suitable for use as control antibody for MTMR9 siRNA (h): sc-61097, phatidylinositol 3-phosphate pathway. Hum. Mol. Genet. 9: 2223-2229. MTMR9 siRNA (m): sc-61098, MTMR9 shRNA Plasmid (h): sc-61097-SH, 3. Kim, S.A., et al. 2003. Regulation of myotubularin-related (MTMR)2 phos - MTMR9 shRNA Plasmid (m): sc-61098-SH, MTMR9 shRNA (h) Lentiviral phatidylinositol phosphatase by MTMR5, a catalytically inactive phospha- Particles: sc-61097-V and MTMR9 shRNA (m) Lentiviral Particles: tase. Proc. Natl. Acad. Sci. USA 100: 4492-4497. sc-61098-V. 4. Mochizuki, Y. and Majerus, P.W. 2003. Characterization of myotubularin- Molecular Weight of MTMR9 : 63 kDa. related protein 7 and its binding partner, myotubularin-related protein 9. Proc. Natl. Acad. Sci. USA 100: 9768-9773. RECOMMENDED SECONDARY REAGENTS 5. Srivastava, S., et al. 2005. Phosphatidylinositol 3-phosphate indirectly To ensure optimal results, the following support (secondary) reagents are activates KCa3.1 via 14 amino acids in the carboxy terminus of KCa3.1. recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 Mol. Biol. Cell 17: 146-154. (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ 6. Berger, P., et al. 2006. Multi-level regulation of myotubularin-related pro tein- Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: 2 phosphatase activity by myotubularin-related protein-13/set-binding fac - sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- tor-2. Hum. Mol. Genet. 15: 569-579. rescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100- 7. Lorenzo, O., et al. 2006. Systematic analysis of myotubularins: heteromer - 1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) ic interactions, subcellular localisation and endosomerelated functions. J. with UltraCruz™ Mounting Medium: sc-24941. Cell. Sci. 119: 2953-9959. RESEARCH USE CHROMOSOMAL LOCATION For research use only, not for use in diagnostic procedures. Genetic locus: MTMR9 (human) mapping to 8p23.1; Mtmr9 (mouse) map ping to 14 D1. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. PROTOCOLS See our web site at www.scbt.com or our catalog for detailed protocols and support products. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.