Neural Cell Markers Neural Cell Markers Neurons and glia in neural tissue or cultures are commonly visualized and identified by immunodetection of cell-specific antigenic markers, including transcription factors, enzymes, cytoskeletal proteins, cell surface proteins, and secreted factors. The Bio-Techne brands R&D Systems and Novus Biologicals together offer an unparalleled selection of antibodies directed against these intracellular and cell surface proteins that can be used for the identification and characterization of different neural cell types. R&D Systems also offers a variety of different immunnoassays, including the gold standard Quantikine® ELISA Kits, for detecting secreted molecules. The Bio-Techne brand Tocris offers a novel and exclusive collection of tools, including bioactive small molecules, caged compounds, and fluorescent probes, for the functional identification of neural cells. Table of Contents Neuronal Markers . 1–8 General Markers. .1 Dendritic Markers. 2 Axonal Markers. 2 Presynaptic Markers. 3 Active Zone Markers. 4 Postsynaptic Markers. .4 Growth Cone Markers. .5 Cholinergic Neuron Markers. 6 Dopaminergic Neuron Markers. .6 GABAergic Neuron Markers. 7 Glutamatergic Neuron Markers. .7 Glycinergic Neuron Markers. 8 Serotonergic Neuron Markers. 8 Microglia Markers. 9–12 Steady-State Microglia Markers. .9 M1 Microglia Markers. .10–11 M2 Microglia Markers. 12 Astrocyte Markers. 13 General Markers. 13 Oligodendrocyte Markers. 14–15 Oligodendrocyte Precursor Cell Markers. .14 Immature Oligodendrocyte Markers . 14 Mature, Non-Myelinating Oligodendrocyte Markers. 15 Mature, Myelinating Oligodendrocyte Markers. 15 Additional Tools for Visualizing and Identifying Neural Cells. 16 Conjugated Primary Antibodies. 16 R&D Systems® VisUCyte™ HRP Polymer. 16 R&D Systems® Multiplex Assays . .16 Tocris® Products to Investigate Neural Function. 17 Small Molecules. 17 Caged Compounds. 17 Photoswitchable Ligands. .17 Fluorescent Probes. 17 Neuronal Markers General Markers Select R&D Systems® and Novus Biologicals® Antibodies Marker Catalog # Species Clonality Applications ◆ Calbindin D AF3320 H Poly WB, IHC ◆ Calbindin D-28K NBP2-50048 H M R + Mono WB, ICC ◆ Doublecortin NBP1-92684 H M R + Mono WB, ICC ◆ Enolase 2/Neuron- MAB5169 H Mono ICC, IHC, IP ◆ Specific Enolase AF5169 H M Poly WB, IHC, SW ◆ NBP1-92711 H M R + Mono WB, ICC MAP2 Nuclear ◆ MAB8304 H Mono ICC, IHC NeuroD1 RBFOX3/NeuN ◆ NeuroD1 AF2746 H M Poly WB, ICC Cytoplasmic ◆ NB300-135 H M R + Poly WB, ICC, IHC, ELISA Calbindin D NF-M NF-H Doublecortin PSD-93 ◆ AF3108 H Poly WB, IHC Enolase 2/Neuron- PSD-95 Specific Enolase ◆ NB300-131 H M R + Poly WB, ICC, IHC Tau NF-L MAP2 b-III Tubulin ◆ MAB2216 H Mono WB, IHC NF-H UCH-L1/PGP9.5 NF-L ◆ NB300-133 H M R + Poly WB, ICC, IHC NF-M ◆ AF3029 H Poly WB, IHC ◆ PSD-93 NB300-546 H M R Poly WB, ICC, IHC ◆ PSD-95 NB300-556 H M R + Mono WB, ICC, IHC, B/N, ChIP, FC, IP ◆ RBFOX3/NeuN NBP1-77686 H M R + Poly WB, ICC, IHC ◆ MAB3494 Ms Mono WB, IHC Tau ◆ AF3494 Ms Poly WB, IHC ◆ β-III Tubulin NB100-1612 H M R Poly WB, ICC, IHC ◆ β-III Tubulin (Clone TuJ-1), Neuron-Specific MAB1195 All Species Mono WB, ICC, SW ◆ UCH-L1/PGP9.5 MAB6007 H Mono WB, IHC, IP, SW NeuN in Mouse Brain. NeuN, also called RBFOX3, was Enolase 2 in Human Brain. Enolase 2, also called Neuron- NF-H in Human Brain. Neurofilament (NF)-H was detected in detected in immersion-fixed paraffin-embedded sections of Specific Enolase, was detected in immersion-fixed paraffin- immersion-fixed paraffin-embedded sections of human brain mouse brain using a Rabbit Anti-Human/Mouse/Rat embedded sections of human brain using a Human/Mouse using a Goat Anti-Human NF-H Antigen Affinity-Purified RBFOX3/NeuN Antigen Affinity-Purified Polyclonal Antibody Enolase 2/Neuron-Specific Enolase Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF3108). The tissue was (Novus Biologicals, Catalog # NBP1-77686). The cerebellar Polyclonal Antibody (Catalog # AF5169). Prior to incubation stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit tissue was stained using HRP and DAB (brown), and with the primary antibody, the tissue was subjected to heat- (Catalog # CTS008; brown) and counterstained with counterstained with hematoxylin (blue). induced epitope retrieval using Antigen Retrieval Reagent- hematoxylin (blue). All cited reagents are from R&D Systems Basic (Catalog # CTS013). The tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. All cited reagents are from R&D Systems. Species Key: H Human M Mouse R Rat Ms Multispecies + Additional Species Available Applications Key: B/N Blocking/Neutralization ChIP Chromatin Immunoprecipitation CyTOF CyTOF-Ready DB Dot Blot ELISA (ELISA Capture and/or Detection) EM Electron Microscopy FA Functional Assay FC Flow Cytometry GS Gel Shift ICC Immunocytochemistry IHC Immunohistochemistry IP Immunoprecipitation IV In Vitro PEP-ELISA Peptide-ELISA RI Radioimmunoassay SW Simple Western™ WB Western blot ◆ Indicates an R&D Systems® antibody ◆ Indicates a Novus Biologicals® antibody Learn more | rndsystems.com/neuralmarkers 1 Dendritic Markers Select R&D Systems® and Novus Biologicals® Antibodies Marker Catalog # Species Clonality Applications ◆ ARC/ARG3.1 NBP1-56929 H Poly WB, IHC ◆ NB100-1951 H M R + Mono WB, ICC, IHC, IP Drebrin 1 ◆ AF7739 H Poly WB, ICC, SW ◆ NBP1-92711 H M R + Mono WB, ICC MAP2 ◆ MAB8304 H Mono ICC, IHC Cytoplasmic ◆ SAP102 NBP1-87691 H M R Poly WB, IHC ARC/ARG3.1 MAP2 Drebrin 1 SAP102 Axonal Markers Select R&D Systems® and Novus Biologicals® Antibodies Marker Catalog # Species Clonality Applications ◆ Kinesin 5A NBP1-76816 H M R Poly WB, ICC, IHC, ELISA ◆ Kinesin 5B NBP2-58451 H M R Poly ICC, IHC ◆ NB100-2682 H M Mono WB, ICC, IHC, CyTOF, ELISA, FC, IP L1CAM ◆ AF277 H Poly WB, IHC ◆ NB300-135 H M R + Poly WB, ICC, IHC, ELISA Cytoplasmic NF-H Kinesin 5A NF-L ◆ AF3108 H Poly WB, IHC Kinesin 5B Peripherin ◆ NB300-131 H M R + Poly WB, ICC, IHC L1CAM Tau NF-L NF-H b-III Tubulin ◆ MAB2216 H Mono WB, IHC Cell Surface ◆ Peripherin NB300-137 H M R + Poly WB, ICC, IHC, ELISA L1CAM ◆ MAB3494 Ms Mono WB, IHC Tau ◆ AF3494 Ms Poly WB, IHC ◆ b-III Tubulin NB100-1612 H M R Poly WB, ICC, IHC ◆ b-III Tubulin (Clone TuJ-1), Neuron-Specific MAB1195 All Species Mono WB, ICC, SW MAP2 in Human Embryonic Stem Cells. Microtubule- bIII Tubulin in Neonatal Mouse Neurons. bIII Tubulin was bIII Tubulin in Differentiated Human Neural Progenitor Associated Protein 2 (MAP2) was detected in immersion- detected in fixed dissociated cell cultures of neonatal Cells. bIII Tubulin was detected in immersion-fixed fixed human embryonic stem cells/neurospheres using a mouse brain using a Chicken Anti-Human/Mouse/Rat bIII differentiated human neural progenitor cells using a Mouse Mouse Anti-Human MAP2 Monoclonal Antibody (Catalog # Tubulin Antigen Affinity-Purified Polyclonal Antibody (Novus Anti-Neuron-Specific bIII Tubulin (Clone TuJ-1) Monoclonal MAB8304). The cells were stained using the NorthernLights™ Biologicals, Catalog # NB100-1612; green). The cells were Antibody (R&D Systems, Catalog # MAB1195). The cells 557-Conjugated Donkey Anti-Mouse IgG Secondary also stained for GFAP (red) and counterstained with DAPI were stained (green) and counterstained (red). Antibody (Catalog # NL007; red) and counterstained with (blue). DAPI (blue). All cited reagents are from R&D Systems. 2 Learn more | novusbio.com/neuroscience Presynaptic Markers Select R&D Systems® and Novus Biologicals® Antibodies Marker Catalog # Species Clonality Applications Postsynatic Neuron ◆ GAP-43 NB300-143 H M R + Poly WB, ICC, IHC ◆ Neurexin 3/NRXN3 AF5269 H M Poly WB, IHC ◆ SNAP23 AF6306 H Poly WB, ICC Presynatic ◆ SNAP25 AF5946 H M R Poly WB, ICC Neuron ◆ SV2A NBP1-82964 H M R Poly WB, ICC, IHC, IP *Cytoplasmic (plasma membrane) ◆ Synapsin I NB300-104 H M R + Poly WB, ICC, IHC, IP GAP-43 SNAP25 ◆ Synapsin II NBP2-58134 H M R Poly ICC SNAP23 Syntaxin 1A ◆ Synaptogyrin 1 NBP1-77371 H M R Poly WB, ICC, IHC, ELISA *Cytoplasmic (synaptic membrane) SV2A Synaptotagmin-1 ◆ NBP2-25170 H M R + Poly WB, ICC, IHC Synapsin I Synaptotagmin-4 Synaptophysin ◆ MAB5555 H Mono ICC, IHC Synapsin II VAMP-1 Synaptogyrin 1 VAMP-2 ◆ NBP1-91499 M + Poly WB, ICC Synaptophysin Synaptotagmin-1 ◆ MAB43641 R Mono WB, IHC, IP Cell Surface ◆ Synaptotagmin-4 NBP2-13408 H Poly WB, ICC, IHC Neurexin 3/NRXN3 VIAAT/SLC32A1/VGAT VGLUT1/SLC17A7 ◆ MAB7237 H Mono WB, IHC Syntaxin 1A *Cytoplasmic marker is found associated with the ◆ AF7237 H M R Poly WB, ICC membrane of the listed cell structure. ◆ VAMP-1 AF4828 H M Poly WB, IHC ◆ VAMP-2 MAB5136 H M Mono WB, IHC ◆ NBP2-46627 H M R Mono WB, IHC VGLUT1/SLC17A7 ◆ MAB9054 H Mono IHC ◆ NBP2-20857 H M R Poly ICC, IHC VIAAT/SLC32A1/VGAT ◆ MAB6847 H Mono ICC Synapsin I in Rat Caudate Neurons. Synapsin I was Synaptotagmin-1 in Rat Spinal Cord. Synaptotagmin-1 was VAMP-1 in Mouse Spinal Cord. Vesicle-Associated detected in fixed rat caudate neurons using a Rabbit Anti- detected in perfusion-fixed frozen sections of rat spinal cord Membrane Protein 1 (VAMP-1), also called Synaptobrevin-1, Human/Mouse/Rat Synapsin I Polyclonal Antibody (Novus using a Mouse Anti-Rat Synaptotagmin-1 Monoclonal was detected in perfusion-fixed frozen sections of mouse Biologicals, Catalog # NB300-104). The cells were stained Antibody (R&D Systems, Catalog # MAB43641). The tissue spinal cord using a Goat Anti-Human/Mouse VAMP-1 (green) and then counterstained for MAP proteins (red). was stained (red) and counterstained (green). Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF4828). The tissue was stained with the NorthernLights™ 557-Conjugated Donkey Anti-Goat IgG Secondary Antibody (Catalog # NL001; red) and counterstained (green).