Volume 51 July 1984 PROCEEDINGS ^ of of Washington '- f, V-i -: ;fx A semiannual journal of research devoted to Helminthohgy and all branches of Parasitology Supported in part by the -•>"""- v, H. Ransom Memorial 'Tryst Fund : CONTENTS -j<:'.:,! •</••• VV V,:'I,,--.. Y~v MEASURES, LENA N., AND Roy C. ANDERSON. Hybridization of Obeliscoides cuniculi r\ XGraybill, 1923) Graybill, ,1924 jand Obeliscoides,cuniculi multistriatus Measures and Anderson, 1983 .........:....... .., :....„......!"......... _ x. iXJ-v- 179 YATES, JON A., AND ROBERT C. LOWRIE, JR. Development of Yatesia hydrochoerus "•! (Nematoda: Filarioidea) to the Infective Stage in-Ixqdid Ticks r... 187 HUIZINGA, HARRY W., AND WILLARD O. GRANATH, JR. -Seasonal ^prevalence of. Chandlerellaquiscali (Onehocercidae: Filarioidea) in Braih, of the Common Grackle " '~. (Quiscdlus quisculd versicolor) '.'.. ;:,„..;.......„.;....• :..: „'.:„.'.J_^.4-~-~-~-<-.ii -, **-. 191 ^PLATT, THOMAS R. Evolution of the Elaphostrongylinae (Nematoda: Metastrongy- X. lojdfea: Protostrongylidae) Parasites of Cervids,(Mammalia) ...,., v.. 196 PLATT, THOMAS R., AND W. JM. SAMUEL. Modex of Entry of First-Stage Larvae ofr _^ ^ Parelaphostrongylus odocoilei^Nematoda: vMefastrongyloidea) into Four Species of Terrestrial Gastropods .....:;.. ....^:...... ./:... .; _.... ..,.....;. .-: 205 THRELFALL, WILLIAM, AND JUAN CARVAJAL. Heliconema pjammobatidus sp. n. (Nematoda: Physalbpteridae) from a Skate,> Psammobatis lima (Chondrichthyes: ; ''•• \^ Rajidae), Taken in Chile _... .„ ;,.....„.......„..,.......;. ,...^.J::...^..,....:.....~L.:....., 208 NICKLE, W. R., R. F. W. SCHRODER, AND J. L. KRYSAN. A^New Peruvian Hexdmermis /' sp. (Nematoda: .Mermithidae) Parasite of-Cbrn Rootworms;^• Diabr&tica spp. .•. 212 ^POINAR, GEORGE 0.,,jR, Daubaylia olsoni sp. n. (Daubayliidae: Rhabditida) from c < the leech, Dina :dnoculata, in California ..... L.......................,.......l._lc._...........l_ ^.. ..„; 217 MILLER, DONALD M., AND T. T. DUNAGAN. A Support'Cell to -the Apical and Lateral Sensory Organs inMoniliformis monilfformis (Acanthocephala) ...;..,„. 221 (Continued on Outside Back Cover)' Copyright © 2011, The Helminthological Society of Washington THE HELMINTHOLOGICAL SOCIETY OF WASHINGTON THE SOCIETY meets once a month from October through !May for the presentation and discussion of papers in any and all branches of parasitology or related sciences. All interested persons are,invited to attend. ' ',-,'. Persons interested in membership in the Helminthplogical Society of Washington may obtain application blanks in recent issues of THE PROCEEDINGS. A year's subscription to the Proceedings is included in the annual dues. President: SHERMAN, S. HENDRIX Vice President: WILLIS A. REID, JR. Corresponding Secretary-Treasurer: MICHAEL D. RUFF Assistant Corresponding Secretary-Treasurer: ROBIN N. HUETTEL Recording Secretary: ' RALPH ECKERLIN _.. X Archivist: DAVID R. LINCICOME Custodian of Back Issues: GERHARD A. SCHAD •/ Librarian: PATRICIA A. PILITT Representative to the" Washington Academy of Sciences: ROBERT S. ISENSTEIN (1976- Representative to the American Society of Parasitologists: RONALD PAYER (1984- ) Executive Committee Members-at-Large: JEFFREY D. BIER, 1984 ROSAMUND W. WENDT, 1984 PATRICK W. CARNEY, 1985 ROY G.TAYLOR, 1985 Immediate Past President: MILFORD N. LUNDE \E PROCEEDINGS OF THE HELMINTHOLOGICAL SOCIETY OF WASHINGTON '••''/ ~ ~ / -..: >THE PROCEEDINGS are published semianhually at Lawrence, Kansas by the Helminthological Society of Washington. Papers need nbt be presented at a meeting to be published in the Proceedings. MANUSCRIPTS should be sent to the EDITOR, J. R. Lichtenfels, USDA, ARS, BARC-East No. 1180, Beltsville, MD 20705. Manuscripts must be typewritten, double spaced, and in finished form: The original'and two copies >are * required. Photocopies of drawings may,be submitted for review purposes but glossy prints of halftones are required;* originals wjlljje requested after acceptance of the manuscript. Papers are accepted with (the understanding that they will be published only in the Proceedings. , ( REPRINTS may be ordered from the PRINTER at the same time the corrected proof is returned to the EDITOR. BACK VOLUMEST>f the Proceedings are available. Inquiries concerning back volumes and current .subscriptions should be directed to the business office. ' N _, \S OFFICE. The Society's business office is at Lawrence, /Kansas. All inquiries concerning subscriptions or back issues and all payments for dues, subscriptions, and back issues should be addressed to: Helminthological Society of Washington, % Allen Press, Inc., 1041 New Hampshire St., Lawrence, Kansas 66044, U.S.A. , y .-'• ./- •, -:-,- T EDITORIAL BOARD v- N ij. RALPH LICHTENFELS, Editor PATRICIA A. PILITT, Assistant Editor 1984 1985 >;.. r.^ "1986 WILLIAM C, CAMPBELL ROY C. ANDERSON /DANIEL R. BROOKS ! - - RALPH ECKERLIN -/I RAYMOND M. CABLE JOHNL.CRITES JOHN C. HOLMES FRANK W. DOUVRES REINO S. FREEMAN JOHN S. MACKIEWICZ RONALD FAYER GILBERT F. OTTO ', BRENT^B. NICKOL A. MORGAN GOLDEN: ROBIN M. OVERSTREET ' MARIETTA VOGE DANNY B. PENCE • HARLEY/G. SHEFFIELD - '-" V,-' - \ Helminthological Society of Washington 1984 Copyright © 2011, The Helminthological Society of Washington Proc. Helminthol. Soc. Wash. 51(2), 1984, pp. 179-186 Hybridization of Obeliscoides cuniculi cuniculi (Graybill, 1923) Graybill, 1924 and Obeliscoides cuniculi multistriatus Measures and Anderson, 1983 LENA N. MEASURES AND ROY C. ANDERSON Department of Zoology, College of Biological Science, University of Guelph, Guelph, Ontario NIG 2W1, Canada ABSTRACT: Obeliscoides cuniculi cuniculi (Graybill, 1923) Graybill, 1924 and Obeliscoides cuniculi multistriatus Measures and Anderson, 1983 were cross-bred in domestic rabbits (Oryctolagus cuniculus). Viable progeny were produced to the F3 generation when female O. c. multistriatus were mated with male O. c. cuniculi. The synlophe (ensemble of enlarged longitudinal or oblique cuticular ridges) was intermediate in character. F[ hybrids had 66 ± 3 (58-73) longitudinal cuticular ridges midbody in females and 46 ± 3 (41-51) in males. The mean number of longitudinal cuticular ridges remained stable in F2 and F3 hybrids, although, the range was greater. Dorsal and ventral ridges near the bursa of males (Fu F2, or F3) were variable in number and position. Eggs resulting from the reciprocal mating (male O. c. multistriatus x female O. c. cuniculi) were not viable; egg shells were distorted and cleavage was unequal and ceased after a few hours. The two subspecies of O. cuniculi are considered incipient species. Obeliscoides cuniculi (Graybill, 1923) Gray- lected over 3 or 4 days. Feces were then placed in bill, 1924 (Trichostrongyloidea) is found in the aluminum trays in an incubator at 26°C, moistened daily, and turned over to break up fungal hyphae. Trays stomach of lagomorphs in North America. Mea- were enclosed in plastic bags to maintain humidity and sures and Anderson (1983a) distinguished two reduce evaporation. After 12 days feces were placed in subspecies, O, cuniculi cuniculi (Graybill, 1923) a Baermann apparatus and infective larvae recovered. Graybill, 1924 in cottontail rabbits (Sylvilagus Rabbits were infected by gastric intubation. Patent in- fections were determined using zinc sulfate flotation of floridanus) and O. cuniculi multistriatus Mea- feces. sures and Anderson, 1983 in snowshoe hares Two rabbits were infected with approximately 2,000 (Lepus americanus). These subspecies can be dif- infective larvae of O. cuniculi multistriatus, and two ferentiated by the synlophe. Wild cottontail rab- rabbits were infected with approximately 2,000 infec- bits examined were infected only with O. c. cu- tive larvae of O. cuniculi cuniculi. Eight days postin- fection rabbits were killed by cervical dislocation and niculi and snowshoe hares only with O. c. worms were recovered by pepsin digestion of the stom- multistriatus (Measures and Anderson, 1983c). ach using a Baermann apparatus at 37°C (Measures However, O. c. cuniculi and O. c. multistriatus and Anderson, 1983b). All worms were fourth-stage develop experimentally in cottontail rabbits, larvae. Males and females of each subspecies were sep- arated, counted, and given by gastric intubation to rab- snowshoe hares, woodchucks (Marmota mo- bits (Table 1). Worms were kept in the pepsin digest nax), and domestic rabbits Oryctolagus cunic- at 37°C during the transfer procedures. ulus (Measures and Anderson, 1983b). The num- Hybrid progeny from successful crossbreeding were ber and arrangement of longitudinal cuticular maintained in rabbits by passage for three generations ridges composing the synlophe remained stable (Table 2). Three of six rabbits infected -with F, or F2 hybrids were killed when infections became patent. when each subspecies was passaged in these hosts Adult hybrids were collected, fixed, and the synlophe (Measures and Anderson, 1983b). The purpose was examined as described in Measures and Anderson of the present study was to determine whether (1983a). The remaining infected rabbits were used as these two subspecies could interbreed. a source of infective larvae for subsequent passage. Eggs discharged in saline or dissected from the uteri of Materials and Methods gravid females, and infective larvae recovered from 12-day fecal cultures, were fixed in hot glycerin alcohol Female New Zealand white rabbits (6-8 wk old) were (1 part glycerin to 9 parts 70% alcohol) and examined. used for experiments. Infective larvae of subspecies of Fecundity