Garimella Sravya Russet Apples

Garimella Sravya Russet Apples

Russet Apples: Look Beyond Looks Transcriptional Regulation of Triterpenes In Russet Apple Skin Sravya Garimella School of Biological Sciences Supervised by: Dr Karine David, Christelle Andre RUSSETING – Beauty Is On The Outside What Causes Russeting? Skin Functions ! New Zealand Apple Fruit Industry - $829 million[1] – exports ! SKIN – protective barrier [2] predominantly red & waxy cultivars like ‘Royal Gala’ ! Environmental and genetic factors – ! Russeting – rough brownish repair patches on skin skin develops microcracks ! Not visually appealing – lower commercial value despite a nutty ! Triterpenes – Secondary metabolites in flavor the apple skin –– machinery ! Contains potent immunomodulatory compounds - controlling their production poorly TRITERPENES understood ! Triterpene composition affected Smooth Skin Russet Skin Research Question ! What controls the production of triterpenes in the apple skin? ! Preliminary data identified KEY GENES expressed in russet skin- MdMYB52, MdMYM66, MdMYB67, MdMYB93, MdNAC038-like ! Check gene expression levels across apples exhibiting different levels of russeting Objectives ! Analyse the varying triterpene composition of the skin ! Functional Analysis – Are one or more genes upregulating the production of specific triterpenes? Triterpene Composition Materials ! Smooth SMOOTH SEMI-RUSSET FULL-RUSSET ! 11 apple varieties – 3 groups based on skin type apples ‘Royal Gala’ ‘Golden Delicious’ ‘Jupps Russet’ produce ! Smooth, Semi-Russet, Fully-Russet Ursanes & Ursanes Oleananes Oleananes ! Russet ‘Cox’s Orange Pippin’ ‘Ribston Pippin’ ‘Wilmont Russet’ Lupanes Apples produce Lupanes too ‘Royal Gala’ ‘Golden Delicious’ ‘Wilmont Russet’ Functional Analysis Why Become a Russet APPLE-OGIST? ! Transcription Factors (TFs) – control expression of ! Develop improved apple varieties with higher triterpene other genes by activating their promoters content ! Triterpenes produced in russet apples – anti-inflammatory, anti- cancer, anti-malarial and anti-viral properties ! CORONAVIRUS ! Lupanes – competitive inhibitor of SARS-Cov2-3CL protease[3] ! Ursanes and Oleananes – Potential as inhibitors of SARS- CoV2 replication according to computer modelling[4] References 1. Aitken, A., & Warrington CNZM, I. (2020). Fresh Facts - New Zealand Horticultural Statistics. Fresh facts.co.nz. Retrieved 14 April 2020, from https://www.freshfacts.co.nz/. 2. Martin, Laetitia & Rose, Jocelyn. (2014). There's more than one way to skin a fruit: Formation and functions of fruit cuticles. Journal of experimental botany. 65. 10.1093/jxb/eru301. 3. Kumar, A., Choudhir, G., Shukla, S. K., Sharma, M., Tyagi, P., Bhushan, A., & Rathore, M. (2020). Identification of phytochemical inhibitors against main protease of COVID-19 using molecular modeling approaches. Journal of biomolecular structure & dynamics, 1–11. Advance online publication. https://doi.org/10.1080/07391102.2020.1772112 4. Gyebi, G. A., Ogunro, O. B., Adegunloye, A. P., Ogunyemi, O. M., & Afolabi, S. O. (2020). Potential inhibitors of coronavirus 3-chymotrypsin-like protease (3CLpro): an in silico screening of alkaloids and terpenoids from African medicinal plants. Journal of biomolecular structure & dynamics, 1–13. Advance online publication. https://doi.org/10.1080/07391102.2020.1764868 Apple image - Rochester Star Nurseries., & Stecher Litho. Co. (1888). [Fruit and flower plates lithographed by the Stecher Lithograph Company]. Rochester, N.Y.: Stecher Litho. Co. Coronavirus image - Centers for Disease Control and Prevention. (n.d.). Retrieved from https://www.cdc.gov/.

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