RT² Profiler PCR Array (96-Well Format and 384-Well [4 x 96] Format) Rabbit Extracellular Matrix & Adhesion Molecules Cat. no. 330231 PANZ-013Z For pathway expression analysis Format For use with the following real-time cyclers RT² Profiler PCR Array, Applied Biosystems® models 5700, 7000, 7300, 7500, Format A 7700, 7900HT, ViiA™ 7 (96-well block); Bio-Rad® models iCycler®, iQ™5, MyiQ™, MyiQ2; Bio-Rad/MJ Research Chromo4™; Eppendorf® Mastercycler® ep realplex models 2, 2s, 4, 4s; Stratagene® models Mx3005P®, Mx3000P®; Takara TP-800 RT² Profiler PCR Array, Applied Biosystems models 7500 (Fast block), 7900HT (Fast Format C block), StepOnePlus™, ViiA 7 (Fast block) RT² Profiler PCR Array, Bio-Rad CFX96™; Bio-Rad/MJ Research models DNA Format D Engine Opticon®, DNA Engine Opticon 2; Stratagene Mx4000® RT² Profiler PCR Array, Applied Biosystems models 7900HT (384-well block), ViiA 7 Format E (384-well block); Bio-Rad CFX384™ RT² Profiler PCR Array, Roche® LightCycler® 480 (96-well block) Format F RT² Profiler PCR Array, Roche LightCycler 480 (384-well block) Format G RT² Profiler PCR Array, Fluidigm® BioMark™ Format H Sample & Assay Technologies Description The Rabbit Extracellular Matrix & Adhesion Molecules RT² Profiler PCR Array profiles the expression of 84 genes important for cell-cell and cell-matrix interactions. Cells attach to proteoglycans and glycoproteins (such as fibronectin, laminin, and collagen) in the extracellular matrix (ECM) substratum via adhesion molecules on their cell surface to define tissue shape, structure, and function. Making and breaking cellular contacts with other cells and the ECM play critical roles in normal processes such as cell growth, division, differentiation, and migration. Disease states (metastasis, rheumatoid arthritis, and cardiovascular and central nervous system disorders) and pathophysiological processes (wound healing and inflammation) also involve ECM remodeling and changes in the classes of cell adhesion molecule expressed on cell surfaces. This array contains ECM proteins including basement membrane constituents, collagens, and other genes defining ECM structure. Matrix and other metalloproteinases that remodel the ECM as well as their inhibitors are also included. This array also represents integrins, selectins, cell-adhesion molecule family members (ICAM, ECAM, NCAM, PECAM, and VCAM), and other genes important to cell adhesion and cytoskeleton bridging such as the catenins. Using real-time PCR, you can easily and reliably analyze expression of a focused panel of genes related to cell adhesion and the ECM with this array. For further details, consult the RT² Profiler PCR Array Handbook. Shipping and storage RT² Profiler PCR Arrays in formats A, C, D, E, F, and G are shipped at ambient temperature, on dry ice, or blue ice packs depending on destination and accompanying products. RT² Profiler PCR Arrays in format H are shipped on dry ice or blue ice packs. For long term storage, keep plates at –20°C. Note: Ensure that you have the correct RT² Profiler PCR Array format for your real-time cycler (see table above). Note: Open the package and store the products appropriately immediately on receipt. Array layout (96-well) For 384-well 4 x 96 PCR arrays, genes are present in a staggered format. Refer to the RT² Profiler PCR Array Handbook for layout. 1 2 3 4 5 6 7 8 9 10 11 12 A ADAMTS1 ADAMTS13 ADAMTS2 ADAMTS8 ANOS1 CD44 CDH1 CLEC3B CNTN1 COL11A1 COL12A1 COL14A1 B COL15A1 COL16A1 COL1A1 COL4A2 COL5A1 COL6A1 COL6A2 COL7A1 COL8A1 CTGF CTNNA1 CTNNB1 C CTNND1 CTNND2 ECM1 FN1 HAS1 ICAM1 ITGA1 ITGA2 ITGA3 ITGA4 ITGA5 ITGA6 D ITGA7 ITGA8 ITGAL ITGAM ITGAV ITGB1 ITGB2 ITGB3 ITGB4 ITGB5 LAMA1 LAMA2 LOC1033464 E LAMA3 LAMB1 LAMB3 MMP1 MMP10 MMP11 MMP12 MMP13 MMP14 MMP15 MMP16 47 F MMP2 MMP3 MMP7 MMP8 MMP9 NCAM1 PECAM1 SELE SELL SGCE SPARC SPG7 G SPP1 TGFBI THBS1 THBS2 THBS3 TIMP1 TIMP2 TIMP3 TNC VCAM1 VCAN VTN LOC1003469 H ACTA2 ACTB GAPDH LDHA NGDC RTC RTC RTC PPC PPC PPC 36 Gene table: RT² Profiler PCR Array Position UniGene GenBank Symbol Description A01 N/A XM_008267152 ADAMTS1 ADAM metallopeptidase with thrombospondin type 1 motif, 1 A02 N/A XM_008253631 ADAMTS13 ADAM metallopeptidase with thrombospondin type 1 motif, 13 A03 N/A XM_008274971 ADAMTS2 ADAM metallopeptidase with thrombospondin type 1 motif, 3 A04 N/A XM_008259140 ADAMTS8 ADAM metallopeptidase with thrombospondin type 1 motif, 8 A05 N/A XM_008251733 ANOS1 Anosmin 1 A06 Ocu.7518 XM_002709048 CD44 CD44 molecule (Indian blood group) A07 N/A XM_002711639 CDH1 E-cadherin-like A08 N/A XM_008260499 CLEC3B C-type lectin domain family 3, member B A09 N/A XM_008259399 CNTN1 Contactin 1 A10 N/A XM_008264806 COL11A1 Collagen, type XI, alpha 1 A11 Ocu.3323 XM_008263112 COL12A1 Collagen, type XII, alpha 1 A12 N/A XM_002710753 COL14A1 Collagen, type XIV, alpha 1 B01 N/A XM_008262238 COL15A1 Collagen, type XV, alpha 1 B02 N/A XM_008273785 COL16A1 Collagen, type XVI, alpha 1 B03 N/A XM_008271783 COL1A1 Collagen, type I, alpha 1 B04 N/A XM_008260180 COL4A2 Collagen, type IV, alpha 2 B05 Ocu.792 XM_008253537 COL5A1 Collagen, type V, alpha 1 B06 N/A XM_008251846 COL6A1 Collagen, type VI, alpha 1 B07 N/A XM_008267252 COL6A2 Collagen, type VI, alpha 2 B08 N/A XM_008260611 COL7A1 Collagen, type VII, alpha 1 B09 Ocu.6202 NM_001082666 COL8A1 Collagen, type VIII, alpha 1 B10 Ocu.3361 XM_008263527 CTGF Connective tissue growth factor B11 Ocu.1707 NM_001171142 CTNNA1 Catenin (cadherin-associated protein), alpha 1, 102kDa B12 Ocu.3227 XM_002713075 CTNNB1 Catenin (cadherin-associated protein), beta 1, 88kDa C01 N/A XM_008271512 CTNND1 Catenin (cadherin-associated protein), delta 1 Catenin (cadherin-associated protein), delta 2 (neural plakophilin-related C02 N/A XM_008262072 CTNND2 arm-repeat protein)-like C03 N/A XM_008264481 ECM1 Extracellular matrix protein 1 C04 Ocu.1875 XM_008259120 FN1 Fibronectin 1 C05 N/A XM_008249800 HAS1 Hyaluronan synthase 1 C06 N/A XM_008250743 ICAM1 Intercellular adhesion molecule-like C07 N/A XM_008262192 ITGA1 Integrin, alpha 1 C08 Ocu.7187 XM_008262193 ITGA2 Alpha 2 integrin C09 N/A XM_008271311 ITGA3 Integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) C10 N/A XM_002712227 ITGA4 Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) C11 Ocu.3321 XM_002711037 ITGA5 Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) C12 Ocu.3337 XM_008258747 ITGA6 Integrin, alpha 6 D01 N/A XM_002711055 ITGA7 Integrin, alpha 7 D02 N/A XM_002717427 ITGA8 Integrin, alpha 8 Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated D03 N/A XM_008248739 ITGAL antigen 1; alpha polypeptide) Position UniGene GenBank Symbol Description D04 N/A XM_002721749 ITGAM Integrin, alpha M (complement component 3 receptor 3 subunit) D05 Ocu.3178 XM_002712329 ITGAV Integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes D06 Ocu.3065 XM_002721189 ITGB1 MDF2, MSK12) D07 N/A XM_002724155 ITGB2 Integrin beta-2-like D08 Ocu.2373 NM_001082066 ITGB3 Integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) D09 Ocu.7185 XM_008250947 ITGB4 Beta 4 integrin D10 N/A XM_002716419 ITGB5 Integrin, beta 5 D11 N/A XM_008261059 LAMA1 Laminin, alpha 1 D12 N/A XM_008263482 LAMA2 Laminin, alpha 2 E01 N/A XM_008261146 LAMA3 Laminin, alpha 3 E02 N/A XM_008258355 LAMB1 Laminin, beta 1 E03 N/A XM_008268459 LAMB3 Laminin, beta 3 LOC103346 E04 N/A XM_008252558 Laminin subunit gamma-1-like 447 E05 Ocu.2695 NM_001171139 MMP1 Matrix metallopeptidase 1 (interstitial collagenase) E06 Ocu.7170 XM_002708561 MMP10 Matrix metalloproteinase 10 E07 Ocu.7169 XM_002724012 MMP11 Matrix metalloproteinase 11 E08 Ocu.2122 NM_001082771 MMP12 Matrix metallopeptidase 12 (macrophage elastase) E09 Ocu.2263 NM_001082037 MMP13 Matrix metallopeptidase 13 (collagenase 3) E10 Ocu.6250 NM_001082793 MMP14 Matrix metallopeptidase 14 (membrane-inserted) E11 N/A XM_002711548 MMP15 Matrix metallopeptidase 15 (membrane-inserted) E12 Ocu.7167 XM_002710677 MMP16 Membrane-type 3 matrix metalloproteinase Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV F01 Ocu.6274 NM_001082209 MMP2 collagenase) F02 Ocu.1964 NM_001082280 MMP3 Matrix metallopeptidase 3 (stromelysin 1, progelatinase) F03 N/A XM_002708573 MMP7 Matrix metallopeptidase 7 (matrilysin, uterine) F04 N/A XM_002708421 MMP8 Matrix metallopeptidase 8 (neutrophil collagenase) Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV F05 Ocu.1773 NM_001082203 MMP9 collagenase) F06 N/A XM_008261250 NCAM1 Neural cell adhesion molecule 1 F07 N/A XM_008271715 PECAM1 Platelet/endothelial cell adhesion molecule 1 F08 Ocu.1871 NM_001082312 SELE Selectin E F09 Ocu.2027 NM_001082352 SELL Selectin L F10 N/A XM_008261715 SGCE Sarcoglycan, epsilon F11 Ocu.544 XM_008255281 SPARC Secreted protein, acidic, cysteine-rich (osteonectin) F12 N/A XM_008252191 SPG7 Spastic paraplegia 7 (pure and complicated autosomal recessive) G01 Ocu.2423 NM_001082194 SPP1 Secreted phosphoprotein 1 G02 Ocu.4839 XM_002710222 TGFBI Transforming growth factor, beta-induced, 68kDa G03 N/A XM_002717799 THBS1 Thrombospondin 1 G04 N/A XM_008252461 THBS2 Thrombospondin-1-like G05 N/A XM_008264353 THBS3 Thrombospondin 3 G06 Ocu.178 NM_001082232 TIMP1 TIMP metallopeptidase inhibitor 1 G07 Ocu.2314 XM_008252508 TIMP2 TIMP metallopeptidase inhibitor 2 G08 Ocu.2315 NM_001195682 TIMP3 TIMP metallopeptidase inhibitor 3 G09 Ocu.7534 XM_008273387 TNC Tenascin C G10 Ocu.3113 NM_001082152 VCAM1 Adhesion molecule VCAM-1 G11 Ocu.2225