Anti-ATM (aa 1950-2050) polyclonal antibody (DPABH-17964) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Antigen Description Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates Ser-139 of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Immunogen Synthetic peptide conjugated to KLH derived from within residues 1950 - 2050 of Human ATM. Isotype IgG Source/Host Rabbit Species Reactivity Human Purification Immunogen affinity purified Conjugate Unconjugated Applications ICC/IF, WB Format Liquid Size 100 μg Buffer Constituents: 1% BSA, PBS, pH 7.4 Preservative 0.02% Sodium Azide 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved Storage Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. GENE INFORMATION Gene Name ATM ataxia telangiectasia mutated [ Homo sapiens ] Official Symbol ATM Synonyms ATM; ataxia telangiectasia mutated; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1; serine- protein kinase ATM; AT mutated; A-T mutated; TEL1, telomere maintenance 1, homolog; Entrez Gene ID 472 Protein Refseq NP_000042.3 UniProt ID A0A024R3C7 Pathway ATM mediated phosphorylation of repair proteins; Apoptosis; Autodegradation of the E3 ubiquitin ligase COP1; BRCA1-associated genome surveillance complex (BASC) Function 1-phosphatidylinositol-3-kinase activity; ATP binding; DNA binding; DNA-dependent protein kinase activity 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.